Antioxidant, antimicrobial and cytotoxic bioassay of Mollugo oppositifolius L

Md. Torequl Islam, Md. Ahad Ali Khan, Jasmin Akther Hossain, Joy Barua

Abstract


The present study was conducted according to the traditional uses of Mollugo oppositifolius L. by the kabiraj (traditional practioner) for the treatment of infectious diseases of the ill fated and poor people of Bangladesh. For this antioxidant, antimicrobial and biolethality potentials were conducted by methanol (MOME), ethanol (MOEE) and petroleum ether (MOPE) extractives of the suspected species. To determine the antioxidant activity the DPPH inhibition method was used. For antimicrobial test, antibacterial and antifungal
sensitivities were performed by disc diffusion method and serial tube dilution method was carried out to determine the minimum
inhibitory concentrations on some human pathogenic bacteria and fungi. For cytotoxicity test, brine shrimp lethality bioassay was conducted. Among the three crude extracts, MOEE produced more significant inhibition of DPPH (IC50; 27 μg/ml); MOPE produced highest zone of inhibition against Bacillus subtilis (16.67 mm) and Microsporum spp. (16.0 mm). On the other hand, MOME produced mild cytotoxicity as 50% and 90% mortality (LC50 and LC90) 8.0 μg/ml and 85.12 μg/ml.
Key words: Antioxidant, antimicrobial, cytotoxicity, Mollugo oppositifolius

Full Text:

PDF

References


WHO. Traditional Medicine: Growing Needs and Potential. WHO

Policy Perspectives on Medicines 2002;1-6.

Apulu N, Dada JO, Odama LE, Galadima M. Antibacterial

activities of aqueous extracts of some Nigerian medicinal plants.

Niger J Bot 1994;45-8.

Adebanjo AO, Adewumi CO, Essien EE. Anti-infective agents of

higher plants. Niger J Biotechnol 1983;8:15-7.

Soerjani M, Kostermans AJ, Tjitrosoepomo G. Weeds of Rice in

Indonesia. Balai Pustaka 1987.

Sahakitpichan P, Disadee W, Ruchirawat S, Kanchanapoom T. L

(-) (N transcinnamoyl)-arginine, an acylamino acid from Glinus

oppositifolius (L.) Aug. DC. Molecules 2010;6186-92.

Inngjerdingen KT, Debes SC, Inngjerdingen M, Hokputsa S,

Harding SE, Rolstad B, et al. Bioactive pectic polysaccharides

from Glinus oppositifolius (L.) Aug. DC., a Malian medicinal

plant, isolation and partial characterization. J Ethnopharmacol

;204-14.

Trease GE and Evans WC. The Text Book of Pharmacognosy. The

Williams & Wilkins Company, Baltimore 2, Maryland, 15th ed.

; p. 64.

AOAC. Official methods of analysis. Association of Analytical

Chemists, 13th ed. Washington DC 1980.

Bhal BS, Bhal A. A Text Book of Organic Chemistry. 13th ed New

Delhi, S. Chand and Company Ltd 1992;5,6,11,12,112-4.

Sadhu SK, Okuyama E, Fujimoto H, Ishibashi M. Separation

of Leucas aspera, a medicinal plant of Bangladesh, guided by

prostaglandin inhibitory and antioxidant activities. Chem Pharm

Bull 2003;595-8.

Bauer AW, Kirby WM, Sherries JC, Tuck M. Antibiotic susceptibility

testing by a standard disc diffusion method. J Am Clin Pathol

;493-6.

Rahman MS, Rashid MA. Antimicrobial activity and cytotoxicity

of eclipta prostrate. Orient Pharm Exp Med 2008;47-52.

Aboaba OO, Smith SI, Olude FO. Antibacterial effect of edible

plant extracts on Escherichia coli 0157:H7. Pak J Nutr 2006;325-7.




DOI: http://dx.doi.org/10.22377/ijgp.v5i2.194

Refbacks

  • There are currently no refbacks.