Antimicrobial, cytotoxic and antidiarrhoeal activity of Fimbristylis aphylla L.

Md. Torequl Islam, Joy Barua, Bijoy Karon, Md. Abdun Noor

Abstract


This study was conducted to evaluate the antimicrobial, cytotoxic and antidiarrhoeal activity of Fimbristylis aphylla L methanol
extract. Preliminary phytochemical screenings with the crude extractives demonstrated the presence of alkaloids, glycosides, saponins and reducing sugars. In the disc diffusion antimicrobial sensitivity test, the crude extractive of the whole plant produced moderate to strong antimicrobial activity against the test microorganisms. The zone of inhibition was found within the range of 10.33–15.33 mm. The strongest zone of inhibition was found against Shigella dysenteriae. The extractive was found active against only a few number of test pathogens. In the cytotoxicity test by brine shrimp lethality bioassay, the extract exhibited moderate cytotoxic activity by 50 and 90% mortality rates as LC50 and LC90 values of 5.87 and 9.33 μg/ml respectively. A moderate dose-dependent antidiarrhoeal activity
was found by the methanol extract of the plant.
Key words: Antidiarrhoeal, antimicrobial, cytotoxic, Fimbristylis aphylla

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References


Saini S, Kaur H, Verma B, Ripudaman, Singh SK. Kigellia Africana

(Lin) Benth-An Overview. Natural Product Radiance 2009;8:190-7.

Monica Naples L, Paul Kessler JA. [Online], Available: http://

www.nationaalherbarium.nl/Riceweedsweb/www/fimbri.htm.

[Last accessed on 2005].

AOAC. Official methods of analysis. Association of Analytical

Chemists, 13th ed. Washington DC; 1980.

Ali M. Text Book of Pharmacognosy. New ed. New Delhi: CBS

Publishers and Distributors; 2009. p.96-97,140,283.

Evans WC. Trease and Evans, Text Book of Pharmacognosy. 15th

ed.; 1934; p. 64.

Finer IL. Organic Chemistry. Vol. 2, 5th ed. England: Longman

Group Ltd.; 1983. p. 518,767.

Bauer AW, Kirby WM, Sheriss JC, Turck M. Antibiotic susceptibility

testing by standardized single method. Am J Clin Pathol

;45:493-6.

Andrews JM. Determination of minimum inhibitory concentrations.

J Antimicrob Chemother 2001;48 Suppl 1:5-16.

Meyer BN, Ferringni NR, Puam JE, Lacobsen LB, Nichols DE,

McLaughlin JL. Brine shrimp: A convenient general bioassay for

active constituents. Planta Med 1982;45:31-4.

Shoba FG, Thomas M. Study of antidiarrhoeal activity of four

medicinal plants in castor oil induced diarrhea. J Ethnopharmacol

;76:73-6.




DOI: http://dx.doi.org/10.22377/ijgp.v5i2.192

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