Preliminary phytochemical investigation and thin layer chromatography profiling of sequential extracts of Moringa oleifera pods

Veena Sharma, Ritu Paliwal


Context: Moringa oleifera Lam (Moringaceae) is a highly valued plant, distributed in many countries of the tropics and subtropics.
It has an impressive range of medicinal uses with high nutritional value. Aim: The present study, primarily aims to carry out a
preliminary phytochemical screening so as to detect the major class of compounds present in M. oleifera and to perform thin layer chromatography (TLC) profiling of all sequential extracts. Materials and Methods: Phytochemical analysis was performed by various qualitative methods and TLC profiling was carried out using various solvent system of varying polarity. Results and Conclusions: Qualitative phytochemical analysis reflects the presence of phenolics, triterpenoids, cardiac glycosides, steroid, alkaloids and saponin in the plant extract. TLC profiling of the M. oleifera pods was carried out using sequential extracts of petroleum ether, benzene, petroleum ether, benzene, chloroform, ethyl acetate, ethanol and water respectively. The results obtained in the present investigation
indicated M. oleifera pods as a rich source of natural antioxidants.
Key words: Moringa oleifera, phytochemical screening, retention factor, thin layer chromatography profiling

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