Anxiolytic activity of aqueous extract of Garcinia indica in mice

Manish Patel, Bhavesh Antala, Chandana Barua, Mangala Lahkar

Abstract


Background: Anxiety or depressed mood is associated with low levels of serotonin in the brain. A hydroxycitric acid (HCA),
constituent of Garcinia indica (GIA), increases serotonin release from isolated rat brain cortex. Aim: To evaluate the anxiolytic
activity of aqueous extract of dried fruits of GIA in mice. Materials and Methods: The anxiolytic‑like effects of aqueous
extract of dried fruits of GIA were evaluated by using elevated plus maze (EPM), hole board and light/dark exploration models
in Swiss albino mice. Control mice were treated with an equal volume of saline, and positive control mice were treated with
diazepam (1 mg/kg). Results: GIA administered orally, 30 min before the test in different doses (125, 250 and 500 mg/kg of
body weight), was able to increase significantly (P < 0.05) the time‑spent and entries into open arms of the EPM and reduced
the time‑spent and entries into closed arms versus control. In the hole‑board test, treatment with GIA (250 and 500 mg/kg)
significantly increased the number of head‑dips and duration of head dipping (P < 0.05). In the light–dark paradigm test,
number of transitions and the time spent in the light box increased with reduction in time spent in the dark box and immobility period significantly (P < 0.05) after treatment with GIA. However, no significant changes in locomotor activity were observed versus control.

Conclusion: The results of the present study suggest that aqueous extract of dried fruits of GIA is an effective
anxiolytic agent for behavioural models in mice.
Key words: Anxiolytic, elevated plus maze, Garcinia indica, light/dark test, locomotor activity


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References


Barua CC, Talukdar A, Begum SA, Borah P, Lahkar M. Anxiolytic

activity of methanol leaf extract of Achyranthes aspera Linn in

mice using experimental models of anxiety. Indian J Pharmacol

;44:63‑7.

Emamghoreishi M, Khasaki M, Aazam MF. Coriandrum sativum:

Evaluation of its anxiolytic effect in the elevated plus‑maze.

J Ethnopharmacol 2005;96:365‑70.

Kirtikar KR, Basu BD. Guttiferae. In: Blatter E, Caius JF,

Mhaskar KS, editors. Indian Medicinal Plants. 2nd ed. Vol. 1.

Calcutta: Prabasi press; 1991. p. 263.

Selvi AT, Joseph GS, Jayaprakasha GK. Inhibition of growth and

aflatoxin production in Aspergillus flavus by Garcinia indica extract

and its antioxidant activity. Food Microbiol 2003;20:455‑60.

Yamaguchi F, Ariga T, Yoshimura Y, Nakazawa H. Antioxidative

and anti‑glycation activity of garcinol from Garcinia indica fruit

rind. J Agric Food Chem 2000;48:180‑5.

Blaine B. Does depression cause obesity? A meta‑analysis of

longitudinal studies of depression and weight control. J Health

Psychol 2008;13:1190‑7.

Wurtman JJ. Depression and weight gain: The serotonin

connection. J Affect Disord 1993;29:183‑92.

Ohia SE, Awe SO, LeDay AM, Opere CA, Bagchi D. Effect of

hydroxycitric acid on serotonin release from isolated rat brain

cortex. Res Commun Mol Pathol Pharmacol 2001;109:210‑6.

Jena BS, Jayaprakasha GK, Sakariah KK. Organic acids from

leaves, fruits, and rinds of Garcinia cowa. J Agric Food Chem

;50:3431‑4.

Mote S, Misra TS. Estimation of hydroxycitric acid (‑)‑HCA

in Garcinia indica Choisy by HPLC method. Anal Chem: An

Indian J [Internet]. 2008 [cited 2009];7. Available from: http://

www.tradescienceinc.com/index.php?option=com_content and

view=article and id=436 and Itemid=104 [Accessed on 2013 May 19].

Odebiyi OO, Sofowora EA. Phytochemical screening of Nigerian

medicinal plants II. Lloydia 1978;41:234‑46.

Adeyemi OO, Yemitan OK, Taiwo AE. Neurosedative and

muscle‑relaxant activities of ethyl acetate extract of Baphia nitida

AFZEL. J Ethnopharmacol 2006;106:312‑6.

Sonavane GS, Sarveiya VP, Kasture VS, Kasture SB. Anxiogenic

activity of Myristica fragrans seeds. Pharmacol Biochem Behav

;71:239‑44.

Jain NN, Ohal CC, Shroff SK, Bhutada RH, Somani RS, Kasture VS,

et al. Clitoria ternatea and the CNS. Pharmacol Biochem Behav

;75:529‑36.

Kulkarni SK. Experiments on intact preparations. In: Kulkarni SK,

editor. Practical Pharmacology and Clinical Pharmacy. 1st ed.

Delhi: Vallabh Publications; 2008. p. 131‑3.

Dawson GR, Tricklebank MD. Use of the elevated plus maze

in the search for novel anxiolytic agents. Trends Pharmacol Sci

;16:33‑6.

Pellow S, Chopin P, File SE, Briley M. Validation of open: Closed

arm entries in an elevated plus‑maze as a measure of anxiety in

the rat. J Neurosci Methods 1985;14:149‑67.

Bhattacharya SK, Satyan KS. Experimental methods for evaluation

of psychotropic agents in rodents: I‑‑Anti‑anxiety agents. Indian J

Exp Biol 1997;35:565‑75.

Treit D. Animal models for the study of anti‑anxiety agents:

A review. Neurosci Biobehav Rev 1985;9:203‑22.

Takeda H, Tsuji M, Matsumiya T. Changes in head‑dipping

behavior in the hole‑board test reflect the anxiogenic and/or

anxiolytic state in mice. Eur J Pharmacol 1998;350:21‑9.

Crawley J, Goodwin FK. Preliminary report of a simple animal

behavior model for the anxiolytic effects of benzodiazepines.

Pharmacol Biochem Behav 1980;13:167‑70.




DOI: http://dx.doi.org/10.22377/ijgp.v7i4.344

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