Evaluation of immunomodulatory activity of ethyl acetate extract of Leucas aspera in Swiss albino mice

Bibin Baby Augustine, Suvakanta Dash, Mangala Lahkar, Venketeswara Rao Amara, Pavan Kumar Samudrala, Jaya Mary Thomas


Background: Leucas aspera is a widely used ethno‑medicinal plant for various diseases in India. In our recent research work, this plant showed powerful anti‑cancer activity in various cancer cell lines by stimulating macrophage cells which plays a central role in the immune system. Objective: The present study was conducted to evaluate the immunomodulatory activity of ethyl acetate extract of aerial parts of Leucas aspera (EALA). Materials and Methods: In the current study, we have used neutrophil adhesion test, carbon clearance test, haemagglutinating antibody titre test, delayed‑type hypersensitivity reaction test and cyclophosphamide‑induced immunosuppression test. EALA 200 and 400 mg/kg oral dose were selected for the study after conducting the acute dose toxicity study. All the studies were performed in Swiss albino mice. Results: EALA showed a dose dependant increase in the neutrophil adhesion
to the nylon fibres, produced a significant increase in the phagocytic index in carbon clearance test and a significant protection against cyclophosphamide‑induced immunosuppression. Moreover both doses of EALA produced an increase in the circulating serum immunoglobulins in haemagglutinating antibody titre test along with an increase in the foot pad edema in delayed‑type hypersensitivity reaction test.

Conclusion: From the above findings it is concluded that EALA has the ability to modulate both humoral and cell‑mediated immunity.
Key words: Cell‑mediated immunity, humoral immunity, immunomodulation, Leucas aspera

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Sharma P. Charaka Samhita, Chikitasasthana. Varanasi:

Chaukhamba Orientalia; 1983. p . 54.

In: Wagner H, Hikino H, Farnsworth, editors. Economic and

Medicinal Plant Research. Vol. 1. London: Academic Press; 1984.

p. 113‑8.

Thatte UM, Dahanukar SA. Ayurveda and contemporary scientific

thought. Trends Pharmacol Sci 1986;7:247.

Wagner H. In: Hikino H, Farnsworth, editors. Economic and

Medicinal Plant Research. Vol. 1. London: Academic Press; 1984.

p. 124‑53.

Chew AL, Jessica JJ, Sasidharan S. Antioxidant and antibacterial

activity of different parts of Leucas aspera. Asian Pac J Trop Biomed


Srinivasan R, Ravali B, Suvarchala P, Honey A, Tejaswini A,

Neeraja P. Leucas aspera Medicinal plant: A review. Int J Pharm

Biosci 2011;2:153‑9.

Kripa KG, Chamundeeswari D, Thanka J, Uma Maheswara

Reddy C. Modulation of inflammatory markers by the ethanolic

extract of Leucas aspera in adjuvant arthritis. J Ethnopharmacol


Thomas L, Asad M, Hrishikeshavan HG, Chandrakala GK. Effect of

centchroman on cellular and humoral immunity. Indian J Physiol

Pharmacol 2007;51:387‑94.

Trease GE, Evans WC. A Text Book of Pharmacognosy. 12th ed.

Oxford: ELSB Baillere Tindal; 1983. p. 23‑30.

Aiyegoro OA, Okoh AI. Preliminary phytochemical screening and

in vitro antioxidant activities of the aqueous extract of Helichrysum

longifolium DC. BMC Complement Altern Med 2010;10:21.

Organisation for Economic Cooperation and Development (OECD).

OECD Guidelines for Testing of Chemicals [Internet]. France:

OECD Publishing; 2006 July 11. Section 4, Health Effects: Test

No. 425: Acute Oral Toxicity: Up‑and‑Down Procedure. Available

from: http://www.oecdbookshop.org/oecd/index.asp/langen. [Last

accessed on 2012 May 24].

Wilkinson PC. Neutrophil adhesion test. In: Vane JK, Ferreria SH,

editors. Handbook of Experimental Pharmacology. 1st ed. Berlin:

Springer Verlag; 1978. p. 10 9.

Gokhale AB, Damre AS, Saraf MN. Investigations into the

immunomodulatory activity of Argyreia speciosa. J Ethnopharmacol


Fulzele SV, Satturwar PM, Joshi SB, Dorle AK. Study of the

immunomodulatory activity of Haridradi ghrita in rats. Indian J

Pharmacol 2003;35:51‑4.

Nelson DS, Mildenhall P. Studies on cytophilic antibodies. 1. The

production by mice of macrophage cytophilic antibodies to sheep

erythrocytes: Relationship to the production of other antibodies

and development of delayed type hypersensitivity. Aust J Exp Biol

Med Sci 1967;45:113‑30.

Ziauddin M, Phansalkar N, Patki P, Diwanay S, Patwardhan B.

Studies on the immunomodulatory effects of Ashwagandha.

J Ethnopharmacol 1996;50:69‑76.

Fulzele SV, Bhurchandi PM, Kanoje VM, Joshi SB, Dorle AK.

Immunostimulant activity of ashtamangal ghrita in rats. Indian J

Pharmacol 2002;34:194‑7.

Atal CK, Sharma ML, Kaul A, Khajuria A. Immunomodulating

agents of plant origin I: Preliminary screening. J Ethnopharmacol


Shinde UA, Phadke AS, Nair AM, Mungantiwar AA, Dikshit VJ,

Saraf MN. Preliminary studies on the immunomodulatory activity

of Cedrus deodara wood oil. Fitoterapia 1999;70:333‑9.

Srikumar R, Jeya Parthasarathy N, Sheela Devi R.

Immunomodulatory activity of triphala on neutrophil functions.

Biol Pharm Bull 2005;28:1398‑403.

Miller LE. In: Ludke HR, Peacock JE, Tomar RH, editors. Manual

of Laboratory Immunology. London: Lea and Febiger; 1991.

p. 1‑18.

Thatte UM, Chhabria SN, Karandikar SM, Dahanukar SA. Protective

effects of Indian medicinal plants against cyclophosphamide

neutropenia. J Postgrad Med 1987;33:185‑8.

Heppner GH, Calabresi P. Selective suppression of humoral

immunity by anti‑neoplastic drugs. Annu Rev Pharmacol Toxicol


DOI: http://dx.doi.org/10.22377/ijgp.v8i2.367


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