Anticandidal activity of Diospyros melanoxylon Roxb. Bark from Similipal Biosphere Reserve, Orissa, India

S. K. Panda, D. Dubey, S. K. Dutta


The anticandidal activity and phytochemicals of bark of Diospyros melanoxylon Roxb. (Ebenaceae) was evaluated against four species of Candida viz. C. albicans, C. krusei, C. parapsilosis and C. tropicalis. Both polar and nonpolar extracts viz. petroleum ether, ethyl acetate, ethanol, methanol and aqueous were prepared and studied for anticandidal activity using agar cup and broth dilution methods. Although all five extracts showed promising anticandidal activity against all tested Candida species, yet maximum activity was observed in ethanol extract. Minimum inhibitory concentration values for most of the extracts ranged from 0.375 to 6.0 mg/ ml, while the least minimum fungicidal concentration value was observed at 3.0-6.0 mg/ml. Phytochemical analysis exhibited the presence of carbohydrates, protein and amino acids, tannin and phenolic compound, glycoside, steroid and sterols and flavonoids
in different extracts. Thin layer chromatography bio-autography showed zones of inhibition 22 mm (C. parapsilosis) and 21 mm (C. tropicalis) with Rf value 0.72 for ethanol extract. These results exhibit the anticandidal activity of D. melanoxylon bark extracts, which may be useful in treatment of candidiasis. However, the active components responsible for antifungal activity need to be evaluated.
Key words: Candidiasis, Diospyros melanoxylon, MIC, plant extract, Similipal Biosphere Reserve.

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