Anticandidal activity of Diospyros melanoxylon Roxb. Bark from Similipal Biosphere Reserve, Orissa, India

S. K. Panda, D. Dubey, S. K. Dutta

Abstract


The anticandidal activity and phytochemicals of bark of Diospyros melanoxylon Roxb. (Ebenaceae) was evaluated against four species of Candida viz. C. albicans, C. krusei, C. parapsilosis and C. tropicalis. Both polar and nonpolar extracts viz. petroleum ether, ethyl acetate, ethanol, methanol and aqueous were prepared and studied for anticandidal activity using agar cup and broth dilution methods. Although all five extracts showed promising anticandidal activity against all tested Candida species, yet maximum activity was observed in ethanol extract. Minimum inhibitory concentration values for most of the extracts ranged from 0.375 to 6.0 mg/ ml, while the least minimum fungicidal concentration value was observed at 3.0-6.0 mg/ml. Phytochemical analysis exhibited the presence of carbohydrates, protein and amino acids, tannin and phenolic compound, glycoside, steroid and sterols and flavonoids
in different extracts. Thin layer chromatography bio-autography showed zones of inhibition 22 mm (C. parapsilosis) and 21 mm (C. tropicalis) with Rf value 0.72 for ethanol extract. These results exhibit the anticandidal activity of D. melanoxylon bark extracts, which may be useful in treatment of candidiasis. However, the active components responsible for antifungal activity need to be evaluated.
Key words: Candidiasis, Diospyros melanoxylon, MIC, plant extract, Similipal Biosphere Reserve.

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Vincent JL, Anaissie E, Bruining H, Demajo W, El-Ebiary M, Haber

J, et al. Epidemiology, diagnosis and treatment of systemic Candida

infection in surgical patients under intensive care. Int Care Med

;24:206-16.

Pfaller MA. Nosocomial candidiasis: Emerging species, reservoirs,

and modes of transmission. Clin Infect Dis 1996;22:589-94.

Pfaller MA, Lockhart SR, Pujol C, Swails-Wenger JA, Messer SA,

Edmond MB, et al. Hospital specificity, region specificity, and

fluconazole resistance of Candida albicans bloodstream isolates. J

Clin Microbiol 1998;36:1518-29.

Pfaller MA, Messer SA, Hollis RJ, Jones RN, Doern GV, Brandt ME,

et al. Trends in species distribution and susceptibility to fluconazole

among blood stream isolates of Candida species in the United

States. Diagn Microbiol Infect Dis 1999;33:217-22.

Beck-Sague C, Jarvis WR. Secular trends in the epidemiology

of nosocomial fungal infections in the United States, 1980–1990:

National Nosocomial Infections Surveillance System. J Infect Dis

;167:1247-51.

Pittet D, Wenzel RP. Nosocomial bloodstream infections. Secular

trends in rates, mortality, and contribution to total hospital deaths.

Arch Intern Med 1995;155:1177-84.

Jarvis WR. Epidemiology of nosocomial fungal infections, with

emphasis on Candida species. Clin Infect Dis 1995;20:1526-30.

Richardson MD, Warnock DW. Fungal Infection: Diagnosis and

Management. London: Blackwell Scientific Publications; 1993.

Goff DA, Koletar SL, Buesching WJ, Barnishan J, Fass RJ. Isolation

of flucanozole-resistant Candida albicans from HIV-negative

patients never treated with azoles. Clin Infect Dis 1995;20:77-83.

Nolte FS, Parkinson T, Falconer DJ. Isolation and charecterisation

of flucanazole and amphoterecin B resistant Candida albicans

from blood of two patients with leukemia. Antimicrob Agents

Chemother 1997;41:196-9.

Panda SK, Thatoi HN, Dutta SK. Antibacterial activity and

phytochemical screening of leaf and bark extracts of Vitex negundo

L. from Similipal Biosphere Reserve, Orissa. J Med Plant Res

;3:294-300.

Saxena HO, Brahmam M. The flora of Similipahar (Similipal),

Orissa. Regional Research Labarotory. Bhubaneswar: CSIR

Publication; 1989.

Pandey AK, Rout SD. Medicinal Plants of Similipal Biosphere

Reserve. In: Das AP editors. Perspectives of Plant Biodiversity.

Dehra Dun: Bishen Singh Mahendra Pal Singh; 2002. p. 681-96.

Rath SK, Mohapatra N, Dubey D, Panda SK, Thatoi HN, Dutta SK.

Antimicrobial activity of Diospyros melanoxylon bark from Similipal

Biosphere Reserve, Orissa, India. Afr J Biotechnol 2009;8:1924-8.

Thatoi HN, Panda SK, Rath SK, Dutta SK. Antimicrobial activity

and ethnomedicinal uses of some medicinal plants from Similipal

Biosphere Reserve, Orissa. Asian J Plant Sci 2008;7:260-7.

Mallavadhani UV, Mahapatra A, Jamh K, Reddy PS. Antimicrobial

activity of some pentacyclic triterpines and their synthesizes

-O-lipophilic chains. Biol Pharm Bull 2004;27:1576-9.

Trease GE, Evans WC. Text book of Pharmocognosy. 3rd ed.

London: Bailliere Tindal; 1989. p. 81-90, 268-98.

Barry AL. Procedure for testing antimicrobial Agents in Agar

media. In: Lorin V, editors. Antibiotics in Laboratory Medicine.

USA: Williams Wilkins Co. Baltimore; 1980. p. 1-23.

Eloff JN. A sensitive and quick microplate method to determine the

minimal inhibitory concentration of the plant extracts for bacteria.

Planta Medica 1998;64:711-3.

Runyoro DK, Matee MI, Ngassapa OD, Joseph CC, Mbwambo ZH.

Screening of Tanzanian medicinal plants for anticandidal activity.

BMC Comp Alt Med 2006;6:11-21.

Edwards JE, Bodey GP, Bowden RA, Buchner T, de Pauw BE,

Filler SG, et al. International conference for the development of a

consensus on the management and prevention of severe candidal

infections. Clin Infect Dis 1997;25:43-59.

Jarvis WR. Epidemiology of nosocomial fungal infections, with

emphasis on Candida species. Clin Infect Dis 1995;20:1526-30.

Edmond MB, Wallace SE, McClish DK, Pfaller MA, Jones RN,

Wenzel RP. Nosocomial bloodstream infections in United States

hospitals: A three-year analysis. Clin Infect Dis 1999;29:239-44.

Viscoli C, Girmenia C, Marinus A, Collette L, Martino P, Vandercam

B, et al. Candidemia in cancer patients: A prospective, multicenter

surveillance study by the invasive fungal infection group (IFIG) of

the European Organization for Research and Treatment of Cancer

(EORTC). Clin Infect Dis 1999;28:1071-9.

Georgopapadakou NH, Walsh TJ. Antifungal agents:

Chemotherapeutic targets and immunologic strategies. Antimicrob

Agents Chemother 1996;40:279-91.

Kao AS, Brandt ME, Pruitt WR, Conn LA, Perkins BA, Stephens

DS, et al. The epidemiology of candidemia in two United States

cities: Results of a population-based active surveillance. Clin Infect

Dis 1999;29:1164-70.

Hejgaard J, Jacobsen S, Svendsen IB. Two antifungal thaumatin-like

proteins from Barley grain. FEBS Lett 1991;291:127-31.

Rauha JP, Remes S, Heinonen M, Hopia A, Kähkönen M, Kujala

T, et al. Antimicrobial effects of Finnish plant extracts containing

flavonoids and other phenolic compounds. Int J Food Microbiol

;56:3-12.

Fevell A, Kemertelidze E, Benidze M, Fallague1 K, Regli P.

Antifungal activity of steroidal glycosides from Yucca gloriosa L.

Phytother Res 2005;19:158-61.




DOI: http://dx.doi.org/10.22377/ijgp.v4i2.129

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