Free radical scavenging activity of aqueous extract of roots of Baliospermum montanum Muell-Arg

Prajakta V. Desai, Raju R. Wadekar, Girish H. Kedar, Kalpana S. Patil


Free radical scavenging potential of the aqueous extract of roots of Baliospermum montanum Muell-Arg, a medicinal plant, was
evaluated by using diphenyl-picryl-hydrazyl (DPPH) assay and nitric oxide (NO) scavenging assay. Th e in vitro antioxidant activity of the plant extract was determined by DPPH and NO scavenging activity method. In the DPPH method, antioxidants present in the plant extract reacted with DPPH, which is a stable free radical, and converted it to 1,1-diphenyl-1,2-picryl, hydrazine. Th e results were expressed as IC50. Ascorbic acid, which was used as a standard, showed an IC50 of 19.90 ± 2.3 μg/ml, whereas the plant extract showed an IC50 of 26.22 ± 0.8 mg/ml. In the second method, NO generated from sodium nitroprusside in aqueous solution at physiological
pH interacted with oxygen to produce nitrite ion, which was measured at 540 nm. Ascorbic acid, the standard, showed an IC50 of 5.62 ± 2.5 μg/ml; the plant extract showed an IC50 of 36.66 ± 2.8 μg/ml. In the present study, aqueous extract of B. montanum showed remarkable and concentration-dependent free radical scavenging activity comparable to that of a standard such as ascorbic acid in the studied models; signifi cant results were observed in the estimated parameters, thereby indicating that B. montanum has promising free radical scavenging activity.
Key words: Baliospermum montanum, DPPH assay, free radical scavenging, nitric oxide assay

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