Flow cytometry analysis of Dooshivishari agada-induced cell cycle arrest in human T-cell acute lymphocytic leukemia (Jurkat) cell lines

Abstract

Introduction: Dooshivishari agada is a herbo-mineral formulation that has been widely prescribed in the
treatment of ~Dooshivisha (cumulative poison), its complications, and also in the management of various other
types of poisoning conditions. ~Dooshivisha (cumulative poison) is a unique type of poisoning explained in
Ayurveda classics, which is caused due to the cumulative deposition of various toxins in small quantities for a
long period. This is more evident in the present era of modernization and urbanization. These deposited toxins
are not fatal but cause diseases such as cancer, oxidative stress, and Parkinsonism. Leukemia is a type of cancer
that has causative factors such as radiation, pesticides, and chemicals. The causative factor and pathology of
leukemia are similar to ~Dooshivisha (cumulative poison). Cell cycle analysis helps to assess the cytotoxic
potentials of different components by assessing the normal cancer cell cycle arrest. The present study was
designed to elucidate the cell cycle arrest induction by methanolic extract of D. agada, in the human T-cell
acute lymphocytic leukemia (JURKAT) cell line. Materials and Methods: Cells were incubated with different
concentrations of methanolic extract of D. agada, and the percentage of cells in various stages of the cell cycle in
compounds treated and untreated populations was determined using FACS. Results: The treatment of cells at the
concentrations of 80 μg/ml and 160 μg/mL of sample D. agada has shown G2M arrest from 5.58% (control) to
1.47% and 12.31%, respectively. S-phase arrest was found to be 13.54% and 15.51% at the same concentrations.
Discussion and Conclusion: This laboratory study demonstrated the cytotoxic effect of the sample methanolic
extract of D. agada through cell cycle arrest.