Protective effect of Nigella sativa against carbon tetrachlorideinduced acute liver injury in experimental rabbit models

Rafi A. M. Al-Razzuqi, Jinan A. Al-Hussaini, Ali A. Al-Jeboori


Acute liver injury is a serious state of extensive damage of liver tissue caused by various reasons. In traditional medicine, certain
medicinal plants have been used to cure and prevent some liver diseases. The objective of this study was to evaluate the hepatoprotective activity of oil extract of Nigella sativa seeds in rabbit models with induced acute liver injury. Carbon tetrachloride (CCl4) was used to induce hepatotoxicity at a dose of 1.25 ml/kg as a mixture with olive oil. N. sativa oil extract was administered at a dose of 0.2 ml/kg/day orally for 7 days. The hepatoprotective effect was assessed by liver function tests and histopathological sections of the liver. Significant reduction in the liver damage was found in animals treated with the extract, as indicated by low levels of serum enzymes, serum bilirubin and improvement of serum protein. Besides, restoration of hepatocellular architecture was evident, as indicated by the presence of normal hepatic vessels, absence of necrosis, and fatty infiltration. The oil extract of N. sativa seeds
showed significant hepatoprotective activity.
Key words: Black cumin, hepatocellular architecture, nigellone, thymoquinone

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Sabaté M, Ibáñez L, Pérez E, Vidal X, Buti M, Xiol X, et al. Risk of

acute liver injury associated with the use of drugs: A multicenter

population survey. Aliment Pharmacol Ther 2007;25:1401-09.

Subramoniam A, Pushpangadan P. Development of phytomedicines

for liver diseases. Indian J Pharmacol 1999;31:166-75.

Masuda T. Domestication of plants in the Old World 3rd edition.

Oxford: Oxford University Press; 2000. p. 206.

Ali BH, Blunden G. Pharmacological and toxicological properties

of Nigella sativa. Phytother Res 2003;17:299-305.

Basu S. Carbon tetrachloride-induced lipid peroxidation: Eicosanoid

formation and their regulation by antioxidant nutrients. Toxicology


David B, Hoffman L, Daniel WW. Herbal extract analyses. 3rd ed.

Canada: John Wiley and Sons; 1993. p. 124-36.

Corl AB, Ashwood ER. Tietiz text book of clinical chemistry. 3rd ed,

Vol. 2. New York: WB Sanders Comp; 1999. p. 1059-60.

Putt N, Fredrick A. Manual of histopathological staining methods.

New York: John Wiley and sons; 1972. p. 335.

Woolson RF. Statistical Methods for the Analysis of Biomedical

Data. New York: John Wiley and Sons; 1987. p. 123-9.

Reckengel RO, Glende EA Jr, Dolak JA, Waller RL. Mechanism of

carbon tetrachloride toxicity. Pharmacol Ther 1989;43:139-54.

Hesham RE, Shgeru N. Chemistry of Bioflavonoids. Indian J Pharm

Educ 2002;36:191-4.

Jeong HG, You HJ, Park SJ, Moon AR, Chung YC, Kang SK, et al.

Hepatoprotective effects of 18beta-glycyrrhetinic acid on carbon

tetrachloride-induced liver injury: Inhibition of cytochrome P450

E1 expression. Pharmacol Res 2002;46:221-7.



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