The protective role of Benincasa hispida on nimesulide - induced hepatotoxicity in albino rat model

Shyamal K. Das, Chandan Roy


The objective of the present investigation was to study the protective role of aqueous extract of pulps of Benincasa hispida (BH) on nimesulide-induced hepatotoxicity model in adult male albino rats. Hepatotoxicity in rats was caused by nimesulide at a dose of 10 mg-/ ml/kg body weight. Hepamerz (L-ornithine, L-aspartate) was administered orally as standard hepatoprotective agent for 14 consecutive days prior to nimesulide treatment at a dose of 10 mg-/ml/kg body weight. This drug has many side effects. These side effects
have prompted the scientific world for the search of alternative herbal remedies of liver damage. In our country BH is easily available, cheap and has no side effects. The aqueous pulp extract of BH was administered orally to rats daily for 14 days before nimesulide treatment. The biochemical parameters were investigated. The results indicated that biochemical changes produced by nimesulide were restored to normal by aqueous extract of pulps of BH. The aqueous pulp extract of BH showed significant hepatoprotective effect through the modulation of antioxidant-mediated mechanism by altering serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, alkaline phosphatase, superoxide dismutase and catalase activities and reduced glutathione and lipid peroxidation levels against nimesulide-induced hepatotoxicity model in rats.
Key words: Benincasa hispida, Hepamerz, hepatotoxicity, nimesulide

Full Text:



Preussmann R. Hepatocarcinogens as potential risk for human

liver cancer. In: Remmer H, Bolt HM, Bannasch P, editors. Primary

liver tumors. Lancaster: MTP Press; 1978. p. 11–29.

Bianchi M, Broggini MA. Clinical trial compairing the efficacy of

nimesulide celecoxib and rofecoxib in osteoartharitis of the knee.

Drugs 2003;63 Suppl 1:37-46.

Jackson L, Robert II, Jason D. Analgesic, anti pyretic and anti

inflammatory agents and drugs employed in the treatment of

gout. Phamacological basis of therapeutics. America: McGraw-Hill

Professional; 2002. P. 687-727.

Solomon D, Glynn R, Mogoun H. Cardiovascular outcomes in

new users of coxibs and nonsteroidal anti inflammatory drugs.

Artharitis Rheum 2006;54:1378-89.

Morrison BW, Daniel SE, Kotey P. Rofecoxib a specific cyclogenase-2

inhibitor in primary - dysmanorhoea - A randomized controlled

trial. Obstet Gynecol 1999;94:504-8.

Laporte JR. Upper gastrointestinal bleedings associated with the

use of NSAIDs. Drug Saf 2004;27:411-20.

Dinchuk JE, Car BD, Focht RJ. Renal abnormalities and

altered inflammatory response in mice lacking COX-II. Nature


Rainsford KD. An analysis from clinico-epidemiological data

of the principal - adverse events from the COX-2 selective

NSAID, nimesulide, with particular reference to hepatic injury.

Inflammopharmacology 1998;6:203-21.

Fossaluzza V, Montagnani G. Efficacy and tolerability of nimesulide

in elderly patients with osteoarthritis: Double-blindtrial versus

naproxen. J Int Med Res 1989;17:295-303.

McCormick PA, Kennedy F, Curry M, Traynor O. COX-2 inhibitor

and fulminant hepatic failure. Lancet 1999;353:40-1.

Hawkey CJ. COX-2 inhibitors. Lancet 1999;353:307-14.

Tan HH, Ong WM, Lai SH, Chow WC. Nimesulide induce

hepatotoxicity and fatal hepatic failure. Singapore Med J


Rodrigo L, De FR, Perez-Pariente JM, Andrade JR. Nimesulide

induced severe hemolytic anemia and acute and severe hemolytic

liver failure leading to liver transplantation. Scand Gastroentrol


Maryam S, Shahzad AW, Ahmad S, Bhatti AS, Aziz K. Combination

therapy of isoniazid and hepamerz (L-ornithine, L-aspartate)

effects on liver and kidney functions of rabbits. Special edition.

Annals 2010;16:95-9.

Malhotra SC. Pharmacological investigation of certain medicinal

plants and compound formulations used in Ayurveda and Siddha.

st ed. Gwalior: Central Council for Research in Ayurveda and

Siddha; 1996. p. 209-326.

Ansari NA. Anthelmintic activity of Benincasa hispida seeds–a

preliminary study (Abstr.). Indian J Pharmacol 1981;13:89.

Chopra RN. Glossary of Indian Medicinal Plants. New Delhi: CSIR,

p: 35.

Yashizumi S, Murakam T, Kadoya M, Matsuda H, Yamahara J,

Yoshikava M. Histamine release inhibitors from was guard the fruits

of Benincasa hispida (thunb). Yakugaku Zassi 1998;118:188-92.

Roy C, Ghosh TK, Guha D. The antioxidative role of Benincasa

hispida on colchicine induced experimental rat model of

Alzheimer’s disease. Biogenic Amines 2007;21:44-57.

Kind PR, King EJ. Method of practical clinical biochemistry. In:

Invarley H, Gowenlock AH, editors. London: Bell Meditors; 1980.

p. 899-900.

Reitman S, Frankel SA. Colourimetric method for the determination

of serum glutamic oxalacetic and glutamic pyruvic transaminases.

Am J Clin Pathol 1957;28:56-63.

Mishra HP, Fridovich I. The generation of radical during superoxide

auto oxidation of hemoglobin. J Biol Chem 1972;34:30-7.

Bhattacharya SK, Bhattacharya A, Das K, Muruganandam AV,

Sairam K. Further investigations on the antioxidant activity of

Ocimam sanctum using different paradigms of oxidative stress

in rats. J Nat Rem 2001;1:6-16.

Cohen G, Dembiec D, Marcus J. Measurement of Catalase activity

in tissue extracts. Anal Biochem 1970;34:30-7.

Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys


Dianzani MU. Lipid peroxidation in ethanol poisoning: A critical

reconsideration. Alcohol 1985;20:161-73.

Husain K, Somani SM. Interaction of exercise and ethanol on

hepatic and plasma antioxidant system in rat. Pathophysiology


Venkateswaran S, Pari L. Effect of Coccinia indica leaves on

antioxidant status in streptozotocin-induced diabetic rats.

J Ethnopharmacol 2003;84:163-68.

Riviere S, Birlouez--Aragon I, Nourhashemi F, Vellas B. Low

plasma vitamin C in Alzheimer patients despite an adequate diet.

Int J Geriatr Psychiatry 1998;13:749-54.

Reiter RJ. Melatonin: Lowering the high price of free radicals.

News Physiol Sci 2000;15:246-50.

Schulz JB, Lindenau J, Seyfried J, Dichgans J. Glutathione,

oxidative stress and neurodegeneration. Eur J Biochem


DiCarlo G, Mascolo N, Izzo AA, Capasso F. Flavonoids: Old

and new aspects of a class of natural therapeutic drugs. Life Sci


Oh H, Kim DH, Cho JH, Kim YC. Hepatoprotective and

free radical scavenging activities of phenolic petrosins and

flavonoids isolated from Equisetum arvense. J Ethnopharmacol




  • There are currently no refbacks.