Extraction of Ashwagandha by conventional extraction methods and evaluation of its anti-stress activity

H. Jain, S. D. Parial, E. Jarald, Anwar S. Daud, Showkat Ahmad


The present study was conducted to compare the yield and the antistress activity of Ashwagandha (Withania somnifera) extracts,
using two extraction methods: hot continuous percolation and maceration. Various parameters like temperature, extraction time (10 hours) solvents (water, alcohol, hydroalcohol) and drug-solvent ratios (1:6, 1:8, 1:10) were fixed. The highest yield was found to be 16.96% w/w by maceration process using water (1:8). The activity of different extracts was done by Plus-Maze model using alprazolam as the standard drug. Significant result was found in water extract and hydroalcoholic (1:8) extract prepared by maceration method and also in hydro-alcoholic extract (1:8) prepared by soxhlet process.
Key words: Alprazolam, anti-stress activity, elevated plus maze, extraction, withania somnifera

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DOI: http://dx.doi.org/10.22377/ijgp.v4i3.143


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