Analgesic and anti-inflammatory activities of the hydroalcoholic extract from Gloriosa superba Linn

Jomy C. John, Jennifer Fernandes, Tanaji Nandgude, Samir R. Niphade, Alok Savla, Pradeep T. Deshmukh

Abstract


Gloriosa supberba (family: Liliaceae) is widely used as a medicinal plant, and the alkaloids from the plant (Colchicines and Gloriosine)are used in the treatment of gout and rheumatism. We evaluated the analgesic and anti-inflammatory activities of hydroalcoholic extract (50% v/v) of dried aerial parts of G. superba. The analgesic activity of the extract was evaluated by using Eddy’s hot plate method and acetic acid-induced writhing method. The anti-inflammatory activity was evaluated by using the cotton wool granuloma model and the carrageenan-induced paw edema model. The percentage inhibitions of writhes or percentage protection were found to be 64.09%,
78.56% and 81.45% for extract at a dose 100, 200 and 400 mg/kg body weight, respectively, in the acetic acid-induced writhing method (P <0.01) when compared with control. The percentage increase in reaction time at 90 minutes were 21.02%, 79.96% and 158.05% for extract at a dose of 100, 200 and 400 mg/kg body weight, respectively, in Eddy’s hot plate method (P <0.01) when compared with control. The percentage inhibition of paw edema was increased with time and gave maximum effect at 2 hours, then declined in case of standard extract 400 mg/kg body weight. Only the 200 and 400 mg/kg body weight extracts exhibited significant result (P <0.05) when compared with control. The rats exhibited 9.59%, 28.72% and 45.8% inhibition of granuloma mass formation after the 7 days treatment with 100, 200 and 400 mg/kg body weight of extract when compared with control (P <0.05) in cotton pellet granuloma.
Key words: Gloriosa supberba, analgesic activity, anti-inflammatory activity

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References


Mali RG, Hudiwale JC, Gavit RS, Patil DA, Patil KS. Herbal

abortifacients used in north Maharashtra. Nat Prod Radiat

;5:315-8.

Kirthikar KR, Basu BD. Indian medicinal plants. 2nd ed. Allahabad:

Popular Publication; 1935. p. 2525-6.

Nadkarni KM. Indian Materia Medica, 3rd ed. Mumbai: Popular

Prakashan; 1996. p. 579.

OECD/ OCD. 425 OECD Guidelines for testing of chemicals acute

oral toxicity up and down procedure 2001;26:1-26.

Turner RA. Screening method in pharmacology. New York:

Academic Press; 1965. p. 152.

Argal A, Pathak AK. CNS activity of Calotropis gigantea roots. J

Ethnopharmacol 2006;106:142–5.

Winter CA, Riely EA, Nuss GW. Carragenan induced edema in

hind paw of the rat as assay for anti-inflammatory drugs. Proc Soc

Exp Biol Med 1962;111:544-7.

Goldstein SA, Shemano I, Daweo R, Betler JM. Cotton pellet

granuloma pouch method for evaluation anti-inflammatory

activity. Arc Intl Pharmcodyn Ther 1967;165:294-301.

Koster R, Anderson M, Debeer E. Acetic acid for analgesic

screening. Fed Proc 1959;18:412.

Duarte ID, Nakamura M, Ferreira SH. Participation of the

sympathetic system in acetic acid-induced writhing in mice. Braz

J Med Biol Res 1988; 21:341–3.

Sharma S, Jain NK, Kulkarni SK. Inhibition of COX-1 enzyme

potentiates opoid induced antinociception in animal model of

central noiception. Indian J Pharmacol 2003;35:21-6.

Della LA, Tubaro A, Dri P, Zilli C, Del NP. The role of flavonoids

in the anti-inflammatory activity of Chamomaiia recutita. Clin

Biol Res 1968;213:481-6.

Dirosa M. Biological properties of carrageenin. J Pharm Pharmacol

;24:89-102.

Chawla AS, Singh M, Murthy MS, Gupta MP, Singh H. Antiinflammatory

action of ferulic acid and its esters in carrageenan

induced rat paw edema model. Indian J Exp Biol 1987; 25:187-9.

Lino CS, Taveira ML, Viana GS, Motos FJ. Analgesic and

anti-inflammatory activity of Justicia pectoralis and its main

constituents: Coumarine and umbelliferone. Phytother Res

;11:211-5.

Al–Rahaily AJ, EI-Tahir KE, Mossa JS, Rafatullah S. Pharmacological

studies of various extract and the major constituents, Iupeol,

obtained from hexane extract of Teclea nobilis in rodent. Nat Prod

Sci 2001;7:76-82.




DOI: http://dx.doi.org/10.22377/ijgp.v3i3.86

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