Evaluation of wound‑healing effect of Ziziphus mauritiana L. leaf extract in rats

Meera Sumanth, Y. R. Bhargavi

Abstract


Background: Ziziphus mauritiana L. leaves are used traditionally for burns, wounds, inflamed parts, hypertension and as a diuretic.
Objective: To evaluate the wound‑healing property of ethanol extract of Ziziphus mauritiana L. leaf (EEZM) on rats using excision, incision and dead space wound models. Materials and Methods: Wistar albino rats (150 − 200 gm) were divided into four groups (n = 6). Group I (control) animals received no treatment, group II animals were given EEZM 300 mg/kg orally, group III animals were applied EEZM gel, 100 mg/cm2 and group IV animals were applied Framycetin skin cream, 100 mg/cm2 topically. In incision and dead space wound models, the treatment was given for 10 days only from the day of wound, skin‑breaking strength and dry granulation tissue weight were estimated in incision wound model. Hydroxyproline content was estimated in dead space wound model. In excision
wound model, treatment continued till the complete healing of the wound. Rate of wound contraction and period of epithelisation was evaluated thereafter. The data was analyzed by one‑way ANOVA, followed by Dunnett’s test. P <0.05 is considered significant.
Results: EEZM increased the rate of wound contraction, decreased period of epithelisation, increased skin‑breaking strength and
dry granulation tissue weight, elevated hydroxyproline content.
Conclusion: Ziziphus mauritiana possesses wound healing activity.
Key words: Period of epithelisation, skin breaking strength, wound healing, wound contraction, Ziziphus mauritiana

Full Text:

PDF

References


Paul W, Sharma CP. Chitosan and alginate wound dressings:

A short review. Trends Biomater Artificial Organs 2004;18:18‑23.

Douglas M, Alan LM. Support for wound healing. Alter Med Rev

;8:359‑60.

Kumarasamyraja D, Jeganathan NS, Manavalan R. A review on

medicinal plants with potential wound healing activity. Int J

Pharma Sci 2012;2:105‑11.

Jyotsana S, Painuli RM, Gaur RD. Plants used by rural communities

of district of Shahjahanpur, Uttar Pradesh. Ind J Tradit Knowl

;9:798‑803.

Jain SP, Singh SC, Sarika S, Singh J, Mishra NP, Anil P. Hitherto

unreported ethnomedicinal uses of plants of Betul District of

Madhya Pradesh. Ind J Tradit Knowl 2010;9:522‑5.

Jain SK, Sumita S. Traditional uses of some Indian plants among

Islanders of Indian Ocean. Ind J Tradit Knowl 2005;4:345‑57.

Abalaka ME, Mann A, Adeyemo SO. Studies on in‑vitro antioxidant

and free radical scavening potential and phytochemical screening

of leaves of Ziziphus mauritiana L compared with Ascorbic acid.

J Med Genet Genomics 2011;3:28‑34.

Raghvendra D, Kushagra D, Janapati YK, Sridhar C, Jayaveera

KN. Anti‑microbial studies of aqueous, methanolic and saponins

extract of leaf of Ziziphus mauritiana on human vaginal pathogens

causing UTI infection. Int J Pharm Life Sci 2010;1:77‑81.

Abalaka ME, Daniyan SY, Mann A. Evaluation of antimicrobial

activities of two Ziziphus species on some microbial pathogens.

Afr J Pharm Pharmacol 2010;4:135‑9.

Dahiru D, William ET, Nadro MS. Protective effect of Ziziphus

mauritiana leaf extract on carbontetrachloride induced liver injury.

Afr J Biotechnol 2005;4:1177‑9.

Dahiru D, Obidoa O. Pretretment of albino rats with aqueous leaf

extract of Ziziphus mauritiana L protects against alcohol induced

liver damage. Trop J Pharm Res 2007;6:705‑10.

Hussain A, Khan MN, Sajid MS, Iqbal Z, Khan MK, Abbas RZ, et al.

In vitro screening of Ziziphus mauritiana and Terminalia arjuna for

their Anthelmintic activity. J Animal Plant Sci 2011;21:781‑6.

Agarwal PK, Singh A, Gaurav K, Goel S, Khanna HD, Goel RK.

Evaluation of wound healing activity of extracts of plantain

banana (Musa sapientum var paradisiacal) in rats. Ind J Exp Biol

;47:32‑40.

Mohd AK, Amit S, Farheen TB, Gaurav S, Veerana G, Asif H. Study

of wound healing activity of Delonix regia flowers in experimental

animal models. Am J Pharmtech Res 2012;2:380‑90.

Gupta MK, Bhandari AK, Ramesh KS. Pharmacognostical

evaluations of the leaves of Ziziphus mauritiana. Int J Pharmceut

Sci Res 2012;3:818‑21.

Senthil RD, Rajkumar M, Kumarappan CT, Senthil KL. Wound

healing activity of an herbal ointment containing the leaf extract

of Ziziphus maratiana Lam. Afr J Pharm Pharmacol 2013;7:98‑103.

Bambal VC, Wyawahare NS, Turaskar AO, Deshmukh TA.

Evaluation of wound healing activity of herbal gel containing the

fruit extract of Coccinia indica wight and arn. (Cucurbitaceae). Int

J Pharm Pharmaceut Sci 2011;3 Suppl 4:319‑22.

Chitra S, Patil MB, Ravi K. Wound healing activity of Hyptis

suaveolens (L.) Poit (Lamiaceae). Int J Pharm Tech Res 2009;1:737‑44.

Asha B, Nagabhushan A, Shashikala GH. Comparative study of

wound healing activity of topical and oral ocimum sanctum linn

in albino rats. Al Ameen J Med Sci 2011;4:309‑14.

Zhaowu Z, Jing L, Haifeng L, Tian X, Wei Z, Haizhu F, et al. Effect

of the matrices and penetration enhancers in elemene transdermal

drug delivery system. Afr J Pharm Pharmacol 2011;5:879‑86.




DOI: https://doi.org/10.22377/ijgp.v8i4.423

Refbacks

  • There are currently no refbacks.