Antidiabetic potential of Zanthoxylum armatum bark extract on streptozotocin‑induced diabetic rats

Himani Karki, Kumud Upadhayay, Himanshu Pal, Raghunath Singh

Abstract


Objective: Hydromethanolic extract of the bark of Zanthoxylum armatum (HMZA) was evaluated for its antidiabetic and antioxidant activity in streptozotocin‑induced diabetic rats. Materials and Methods: HMZA was evaluated in normal and diabetic rats. Diabetes was induced by streptozotocin (60 mg/kg i.p.). Normal and diabetic rats were divided into different groups and orally administered with HMZA (200 and 400 mg/kg) and glibenclamide (5 mg/kg) for 21 days. Blood samples were collected from overnight‑fasted rats on at 7, 14 and 21 days of treatment and analysed for blood glucose level and lipid profile. On day 21, rats were sacrificed and liver and kidney tissues were excised to measure their antioxidant status. Results: Oral administration of HMZA for 21 days (200 and 400 mg/kg) resulted in significant reduction in blood glucose, total cholesterol, triglycerides, low density lipoprotein, very low density
lipoprotein and significant increase in high density lipoprotein and body weight of streptozotocin diabetic rats. In addition to that, significant decrease in lipid peroxidation and increase in catalase, superoxide dismutase and reduced glutathione were observed in streptozotocin diabetic rats. Conclusion: From the present study, it is evident that, the bark of Z. armatum possesses significant antidiabetic and antioxidant effect on diabetic rats and suggests that the plant may have therapeutic value in diabetes and related complications.
Key words: Antidiabetic, antioxidant, glibenclamide, streptozotocin, Zanthoxylum armatum

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Signorini AM, Fondelli C, Renzoni E, Puccetti C, Gragnoli G,

Giorgi G. Antioxidant effect of gliclazide, glibenclamide and

metformin in patients with type 2 diabetes mellitus. Curr

Therapeutic Res 2002;63:411‑20.

Giugliano D, Ceriello A, Paolisso G. Oxidative stress and diabetic

vascular complications. Diabetes Care 1996;19:257‑67.

Kaneto H, Katakami N, Kawamori D, Miyatsuka T, Sakamoto K,

Matsuoka TA, et al. Involvement of oxidative stress in the

pathogenesis of diabetes. Antioxidants Redox Signal 2007;9:355‑66.

Rolo AP, Palmeira CM. Diabetes and mitochondrial function: Role

of hyperglycemia and oxidative stress. Toxicol Appl Pharmacol

;212:167‑78.

Noiri E, Tsukahara H. Parameters for measurement of oxidative

stress in diabetes mellitus: Applicability of enzyme‑linked

immunosorbent assay for clinical evaluation. J Investig Med

;53:167‑75.

Tiwary M, Naik SN, Tewary DK, Mital PK, Yadav S. Chemical

composition and larvicidal activities of the essential oil of

Zanthoxylum armatum DC (Rutaceae) against three mosquito

vectors. J Vector Borne Dis 2007;44;198‑204.

Mehta MB, Kharya MD, Srivastava R, Verma KC. Antimicrobial

and anthelmintic activities of the essential oil of Zanthoxylum

alatum Roxb. Ind Perfum 1981;25:19‑21.

Kokate SD, Venkatachalam SR, Hassarajani SA. Evaluation of the

insecticidal principle of Z. alatum. Proc Nat Acad Sci India Sec‑B

Biol Sci 2001;71:229‑32.

Gupta S, Kataria M, Gupta PK, Murganandan S, Yashroy RC.

Protective role of extracts of neem seeds in diabetes caused by

Streptozotocin in rats. J Ethnopharmacol 2004;90:185‑9.

Badimon JJ, Badimon L, Fuester V. Regression of atherosclerotic

lesions by HDL plasma fraction in the Cholesterol‑fed rabbit. J Clin

Invest 1990;85:1234‑41.

Lefevre G, Beljean‑Leymarie M, Beyerle F, Bonnefont‑Rousselot D,

Cristol JP, Therond P, et al. Evaluation of lipid peroxidation by

measuring thiobarbituric acid reactive substances. Ann Biol

Clin (Paris) 1998;56:305‑19.

Ellman GL. Tissue Sulphydryl Groups. Arch Biochem Biophys

;82:70‐7.

Kakkar P, Das B, Vishwanathan PN. A modified spectrophotometric

assay of superoxide dismutase. Indian J Biochem Biophys

;21:130‑2.

Luck H. In: Bergmeyer HV, editor. Methods of Enzymatic Analysis,

Vol. 3. New York: Academic Press; 1963. p. 886‑8.

Aragno M, Mastrocola R, Catalano MG, Brignardello E, Danni O,

Boccuzzi G. Oxidative stress impairs skeletal muscle repair in

diabetic rats. Diabetes 2004;53:1082‑8.

Sharma SB, Nasir A, Prabhu KM, Murthy PS, Dev G. Hypoglycaemic

and hypolipidemic effect of ethanolic extract of seeds of Eugenia

jambolana in alloxan‑induced diabetic rabbits. J Ethnopharmacol

;85:201‑6.

Pushparaj PN, Low HK, Manikandan J, Tan BK, Tan CH.

Anti‑diabetic effects of Cichorium intybus in streptozotocin‑induced

diabetic rats. J Ethnopharmacol 2007;111:430‑4.

Sakatani T, Shirayama T, Suzaki Y, Yamamoto T, Mani H,

Kawasaki T, et al. The association between cholesterol and

mortality in heart failure. Comparison between patients with and

without coronary artery disease. Int Heart J 2005;46:619‑29.

Swanston‑Flat SK, Day C, Bailey CJ, Flatt PR. Traditional plant

treatment for diabetes: Studies in normal and streptozotocin

diabetic mice. Diabetologia 1990;33:462‑4.

Chatterjee MN, Shinde R. Text Book of Medical Biochemistry

New Delhi: Jaypee Brothers Medical Publishers; 2002. p. 317.

Kamalakannan N, Prince PS. Anti‑hyperglycaemic and antioxidant

effect of rutin, a polyphenolic flavonoid, in streptozotocin‑induced

diabetic wistar rats. Basic Clin Pharmacol Toxicol 2006;98:97‑103.

Ceriello A. New insights on oxidative stress and diabetic

complications may lead to a “causal” antioxidant therapy. Diabetes

Care 2003;26:1589‑96.

Nakakimura H, Mizuno K. Studies on lipid peroxidation in

biological system. II. Hyperlipoperoxidemia in mice induced by

alloxan. Chem Pharm Bull 1980;28:2207‑11.

Loven D, Schedl H, Wilson H, Daabees TT, Stegink LD, Diekus M,

et al. Effect of insulin and oral glutathione on glutathione levels and

superoxide dismutase activities in organs of rats with streptazocin

induced diabetes. Diabetes 1986;35:503‑7.

Gutteridge JMC. Lipid peroxidation and antioxidant as biomarkers

of tissue damage. Clin Chem 1995;41:1819‑28.

Robertson PR, Harmon SJ. Diabetes, glucose toxicity, and oxidative

stress: A case of double jeopardy for the pancreatic islet β‑cell. Free

Radic Biol Med 2006;41:177‑84.

Loew D, Kaszkin M. Approaching the problem of bioequivalence

of herbal medicinal products. Phytother Res 2002;16:705‑11.




DOI: http://dx.doi.org/10.22377/ijgp.v8i2.366

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