Wound healing activity of the methanol extracts of Clematis species indigenous to Ethiopia

Segewkal Hawaze, Habtewold Deti, Sultan Suleman


Objective: To investigate the vulnerary activity of the methanol extracts of Clematis longicauda steud ex A. Rich, and Clematis
burgensis Engl leaves. Materials and Methods: In vivo wound healing activity of methanol extracts of C. longicauda and C. burgensis were studied using excision wound model, re‑sutured incision wound model, histopathological analysis and anti‑inflammatory activity test. The variables studied were percentage of wound contraction and epithelialisation period in excision wound model; tensile strength in incision wound model; and percentage of acetic‑acid‑induced capillary permeability inhibition in the anti‑inflammatory activity test. The histopathological study qualitatively assessed presence of inflammatory cells, fibroblast proliferation, collagen
formation and angiogenesis. Differences between experimental groups were compared by one‑way analysis of variance, followed by Dunnett’s test. P < 0.0001 was considered statistically significant. Results: C. longicauda and C. burgensis extracts treated animals showed significant reduction in wound area and faster rate of epithelialisation, P < 0.0001. Extracts of these plants also demonstrated statistically significant wound breaking strength and inhibition of vascular permeability induced by acetic acid. Histological studies on granulation tissue sections showed formation of collagen bundles in the C. longicauda and C. burgensis extracts treated and standard drug‑treated groups while inflammatory cells were present in control. Conclusion: Methanol extracts of C. longicauda and C. burgensis had comparable wound healing activity to Madecassol, containing 1% of Centalla asiatica extracts.
Key words: Anti‑inflammatory activity, Clematis burgensis Engl, Clematis longicauda steud ex A. Rich, wound healing activity

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In: Cotran RS, Kumar V, Robbins SL, Schoen FJ, editors.

Inflammation and repair. In: Robbins Pathologic Basis of Disease.

Philadelphia: WB Saunders Company; 1994. p. 86.

Babu BH, Lakshmi SM. Wound healing activity of methanolic

extract of Martynia annua L. (Martyniaceae). Inter J Phytotherapy


Kumar B, Vijayakumar M, Govindarajan R, Pushpangadan P.

Ethnopharmacological approaches to wound healing-exploring

medicinal plants of India. J Ethnopharmacol 2007;114:103‑13.

Buffoni F, Banchelli G, Cambi S, Ignesti G, Pirisino R, Raimondi L.

Skin wound healing: Some biochemical parameters in guinea‑pig.

J Pharm Pharmacol 1993;45:784‑90.

Pierce GF, Mustoe TA. Pharmacologic enhancement of wound

healing. Annu Rev Med 1995;46:467‑81.

Stuart E, Patricia P. Pathophysiology of Healing. London:

Woodhead Publishing and CRC Press; 2004. p. 234.

Wagner AK, Graves AJ, Reiss SK, Lecates R, Zhang F,

Ross‑Degnan D. Access to care and medicines, burden of health

care expenditures, and risk protection: Results from the World

Health Survey. Health Policy 2011;100:151‑8.

Annan K, Houghton PJ. Antibacterial, antioxidant and fibroblast

growth stimulation of aqueous extracts of Ficus asperifolia Miq.

and Gossypium arboreum L., wound‑healing plants of Ghana.

J Ethnopharmacol 2008;119:141‑4.

Gavimath CC, Sudeep HV, Sujan Ganapathy PS, Padmalatha Rai S,

Ramachandra YL. Evaluation of wound healing activity of Butea

monosperma Lam. extracts on rats. Pharmacology Online 2009;2:203‑16.

Nagappa AN, Cheriyan B. Wound healing activity of the aqueous

extract of Thespesia populnea fruit. Fitoterapia 2001;72:503‑6.

Suguna L, Sivakumar P, Chandrakasan G. Effects of Centella

asiatica extract on dermal wound healing in rats. Indian J Exp Biol


Bhaskar A, Nithya V. Evaluation of the wound‑healing activity of

Hibiscus rosa sinensis L (Malvaceae) in Wistar albino rats. Indian J

Pharmacol 2012;44:694‑8.

Hawaze S, Deti H, Suleman S. In vitro antimicrobial activity

and phytochemical screening of Clematis species indigenous to

Ethiopia. Indian J Pharm Sci 2012;74:29‑35.

National Research Council (US) Committee for the update

of the guide for the care and use of laboratory animals.

Washington (DC): National Academies Press (US); 2011. p. 11-88.

Reddy JS, Rao PR, Reddy MS. Wound healing effects of

Heliotropium indicum, Plumbago zeylanicum and Acalypha indica in

rats. J Ethnopharmacol 2002;79:249‑51.

Tumen I, Süntar I, Keleş H, Akkol EK. A therapeutic approach for

wound healing by using essential oils of Cupressus and Juniperus

species growing in Turkey. Evid Based Complement Alternat Med


Bedi MK, Shenefelt PD. Herbal therapy in dermatology. Arch

Dermatol 2002;138:232‑42.

Leite SN, Palhano G, Almeida S, Biavatti MW. Wound healing

activity and systemic effects of Vernonia scorpioides extract in guinea

pig. Fitoterapia 2002;73:496‑500.

Chaudhari M, Mengi S. Evaluation of phytoconstituents of

Terminalia arjuna for wound healing activity in rats. Phytother Res


Trombetta D, Puglia C, Perri D, Licata A, Pergolizzi S, Lauriano ER.

Effect of polysaccharides from Opuntia‑Ficus‑indica (L.) cladodes

on the healing of dermal wounds in the rat. Phytomedicine


Mukherjee PK. Quality control herbal drugs: An approach to

evaluation of botanicals. New Delhi: Business Horizons; 2002.

p. 546‑9.

Shimizu N, Watanabe T, Arakawa T, Fujiwara Y, Higuchi K,

Kuroki T. Pentoxifylline accelerates gastric ulcer healing in rats:

Roles of tumor necrosis factor alpha and neutrophils during the

early phase of ulcer healing. Digestion 2000;61:157‑64.

Zimmerman JJ, Ciesielski W, Lewandoski J. Neutrophil‑mediated

phospholipid peroxidation assessed by gas chromatography‑mass

spectroscopy. Am J Physiol 1997;273:653‑61.

Getie M, Gebre‑Mariam T, Rietz R, Neubert RH. Evaluation of the

release profiles of flavonoids from topical formulations of the crude

extract of the leaves of Dodonea viscosa (Sapindaceae). Pharmazie


Shetty S, Udupa S, Udupa L. Evaluation of antioxidant and wound

healing effects of alcoholic and aqueous extract of Ocimum sanctum

Linn in Rats. Evid Based Complement Alternat Med 2008;5:95‑101.

Goldsmith LA, editor. Cutaneous wound repairs. In: Physiology,

Biochemistry and Molecular Biology of Skin. New York: Oxford

University Press; 1991. p. 576.

DOI: https://doi.org/10.22377/ijgp.v7i4.339


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