Comparative pharmacognosy of medicinal plant species used as Prsniparni

S. Lalitha, S. John Adams, Pisharath Madhavan Deepthi, K. V. Krishnamurthy, Venkatasubramanian Padma

Abstract


Background: Substitution or adulteration of a particular genuine drug with other species due to demand exceeding the supply of
the original species, is rampant in the present trade scenario. As a result, proper authentication of the drug for safe administration
as an herbal medicine assumes paramount significance. Aim: Prsniparni, Uraria picta (Jacq.) DC., is one such drug for which three different botanical entities are commonly used as substitutes, namely U. lagopodoides (L.)DC; Desmodium gangeticum (L.) DC., and Pseudarthria viscida (L.) Wight and Arn.; all belonging to the family Fabaceae. The anatomical, histochemical and powder microscopic characters of the four species were compared in the field‑collected samples to validate the quality herbal drug and to find the similarity
and dissimilarity of the substitute species. Materials and Methods: Histological and histochemical characters were studied using sectioned materials following standard protocols. Histochemical methods were adopted to localize the presence of the primary metabolites such as starch, lipids, total proteins and amino acids and the secondary metabolites such as volatile oils, resins, tannins, lignin and pectin. Results: The present study shows that the authentic species U. picta and substitute species U. lagopodoides showing higher similarities of 90% based on histology, histochemistry and powder microscopy analysis. Other two candidates, D. gangeticum and P. viscida showing 60 % and 55% similarities, respectively, when compared to U. picta. Thus, the similarity matrix were developed using characters based on anatomical, histochemical and powder microscopy. Conclusion: Ayurvedic texts suggest use of substitute herbs for the rare species. The substitution is proved to be logical by our studies that U. lagopodoides can be used as a substitute species
in the place of U. picta under Prsniparni and also the present study validates the genuinity of the drug by anatomical, histochemical as well as powder microscopy characters to quality checking of the raw drug.
Key words: Ayurveda, Desmodium gangeticum, histochemistry, powder microscopy, Pseudarthria viscida, similarity matrix,
Uraria lagopodoides, Uraria picta

Full Text:

PDF

References


Ved DK, Goraya GS. Demand and Supply of Medicinal Plants

in India. In: Singh B, Singh MP. Dehra Dun: Publishers and

Distributors of Scientific Books; 2008 p. 108-115.

Mishra SN, editor. Bhaisajya Ratnavali of Govinda Das Sen.

Varanasi: Chaukhaba Surbharati Prakashan; 2007 p. 780.

Chunekar KC, editor. Bhavaprakasa Nighantu of Bhavamisra,

Varanasi: Chaukhambha Bharati Academy; 2004 p. 286.

Anonymous. The Ayurvedic Pharmacopoeia of India. 1st ed, vol 4.

New Delhi: Government of India, Ministry of Health and Family

Welfare, Department of AYUSH; Part‑ I, 2004 p.113-5.

Sivarajan VV, Balachandran I. Ayurvedic Drugs and Their Plant

Sources. New Delhi: Oxford and IBH Publishing Co. Pvt. Ltd; 1994

p. 415.

Sastry K. editor. Caraka Samhita of Agnivesa with Cakrapanidatta

Tika, Part I, Varanasi: Chaukhambha Sanskrit Sansthan; 1997.

Krishnamurthy KV. Methods in Plant Histochemistry, Chennai: S.

Vishwanath Printers and Publishers Pvt. Ltd; 1988 p.67.

WHO (World Health Organization). Quality Control Methods for

Medicinal Plant Materials, Geneva: World Health Organization;

Marimuthu R. Microscopy and Microtechnique, Chennai: MJP

Publishers; 2008 p. 380-381.

Pisharath DM. Comparative pharmacognostic and phytochemical

analysis of four species commonly used as Prsniparni in Dasamula,

an ayurvedic formulation, M. Sc Dissertation, Department of

Pharmacognosy. Bangalore: India: Al‑Ameen College of Pharmacy;

Billore KV, Yelne MB, Dennis TJ, Chaudhari BG. Database on

Medicinal Plants used in Ayurveda. New Delhi: Central Council

for Research in Ayurveda and Siddha; 2004 p.314.

Carlquist S. Comparative Wood Anatomy. 2nd ed. Berlin:

Springer‑Verlag; 2001.

Anonymous. The Ayurvedic Pharmacopoeia of India. 1st ed, Part‑1,

vol. 3. New Delhi: Government of India, Ministry of Health and

How to cite this article: Lalitha S, Adams SJ, Deepthi PM, Krishnamurthy

KV, Padma V. Comparative pharmacognosy of medicinal plant species used

as Prsniparni. Int J Green Pharm 2012;6:303-9.

Source of Support: Ministry of National Medicinal Plant Board (NMPB),

New Delhi, Grant No: Z. 18017/187/CSS/R&D/KR-02/2009-10-NMPB. Conflict

of Interest: None declared.

Family Welfare. Department of AYUSH; 2001 p. 179-181.

Rajendran K, Srinivasan KK, Shirwaikar A. Pharmacognostical

evaluation of the roots of Pseudarthria viscida (Linn.). Nat Prod Sci

;13:214‑9.




DOI: http://dx.doi.org/10.22377/ijgp.v6i4.279

Refbacks

  • There are currently no refbacks.