Determination of antimicrobial potentialities of different solvent extracts of the medicinal plant: Phyllanthus amarus Schum. and Thonn.

Antara Sen, Amla Batra


The antimicrobial efficiency of Phyllanthus amarus Schum. and Thonn., medicinal plants (leaf extracts), was examined using Methanol, Ethanol, Petroleum ether. and water, as solvents and tested against eight human pathogens like Bacteria: Bacillus cereus, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, Fungi: Aspergillus niger, Aspergillus flavus, Fusarium oxisporum, and Rhizopus
stolonifer, using the agar well-diffusion method and minimum inhibitory concentration. All the plants showed significant activity against all pathogens, but the alcoholic extract of P. amarus showed the maximum zone of inhibition and minimum inhibitory concentration against all the microorganisms. The minimum zone of inhibition and comparatively greater inhibitory concentration were determined in petroleum ether, and the aqueous extract of P. amarus showed less antimicrobial activity against all the experimental strains. The alcoholic extracts of these plants could be a possible source of obtaining new and effective herbal medicines to treat infections, hence,
it justified the ethnic use of P. amarus against various infectious diseases.
Key words: Agar well well-diffusion method, antimicrobial activity, medicinal plants, minimum bactericidal concentration,
minimum fungicidal concentration, minimum inhibitory concentration, Phyllanthus amarus

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Bromilow C. Problem plants of South Africa. Pretoria South Africa:

Briza Publication; 2001.

Schneider A. Wild medicinal plants. Canada: Stackpole Books;

Muthu AK, Sravanthi P, Kumar DS, Smith AA, Manavalan R.

Evaluation of antibacterial activity of various extracts of whole

plant of Borreria hispida (linn). Int J Pharm Sci Res 2010;1:127-30.

Abel C, Busia K. An explanatory ethnobotanical study of the

practice of herbal medicine by the Akan peoples of Ghana. Altern

Med Rev 2005;10:112-22.

Roosita K, Kusharto CM, Sekiyama M, Fachrurozi Y, Ohtsuka R.

Medicinal plants used by the villagers of a Sundanese community

in West Java, Indonesia. J Ethnopharmacol 2008;115:72-81.

Scazzocchio F, Cometa MF, Tomassini L, Palmery M. Antibacterial

activity of Hydrastis canadensis extract and its major isolated

alkaloids. Planta Med 2001;67:561-4.

Iwu MM, Duncan AR, Okunji CO. New antimicrobials of plant

origin. Alexandra: ASHS Press; 1999.

Mahat MA, Patil BM. Evalution of antiinflamantory activity of

methanol extract of Phyllanthus amarus in experimental animal

model. Indian J Pharm Sci 2007;69:33-6.

Kumar SS, Kumar BR, Mohan GK. Antihepatotoxic activity of

Trichosanthes cucumerina on carbon tetrachloride induced liver

damage in rats. Pharmacology online 2007;3:461-9.

Joshi H. Evaluation of the antiamnesic effects of Phyllanthus amarus

in mice. Colomb Méd 2007;38:132-9.

Kiran D, Rohilla A, Rohilla S, Khan MU. Pleiotropic multifaceted

therapeutic potential of Phyllanthus amarus. Int J Pharma Biological

Arch 2011;2:610-4.

Thyagarajan HF, Blumberg B, Chase F. Chanca-piedra’s anti HBV

and anti-viral properties. Indian J Exp Biol 1998;6:76-8.

Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken HR. Manual

of Clinical Microbiology. 6th ed. Washington DC: ASM Press; 1995.

p. 15-8.

Olurinola PF. A laboratory manual of pharmaceutical microbiology.

Idu, Abuja, Nigeria: National Institute for Pharmaceutical Research

and Development; 1996. p. 69-105.

Akinyemi KO, Oladapo O, Okwara CE, Ibe CC, Fasure KA.

Screening of crude extracts of six medicinal plants used in South-

West Nigerian unorthodox medicine for anti-methicillin resistant

Staphylococcus aureus activity. BMC Complement Altern Med


Ahmad I, Beg AZ. Antimicrobial and phytochemical studies on

Indian medicinal plants against multiple drug resistant human

pathogens. J Ethanopharmacol 2001;74:113-23.

Sharma A. Antibacterial activity of ethanolic extracts of some arid

zone plants. Int J Pharm Tech Res 2011;3:283-6.

Geethalakshmi R, Sarada DV, Marimuthu P. Evalution of

antimicrobial and antioxidant potentials of Trianthema decandra

L. Asian J Biotech 2010;2:225-31.

Rahman MS, Salehin MF, Jamal MAHM, Pravin A, Alam.

Antibacterial activity of Argemone mexicana L. against water brone

microbes. Res J Medicinal plant 2011;5:621-6.

Upadhyay RK, Tripathi R, Ahmad S. Antimicrobial activity

of two Indian medicinal plants Tinospora cordifolia (Family:

Menispermaceae) and Cassia fistula (Family: Caesalpinaceae)

against human pathogenic bacteria. J Pharma Res 2011;4:


Cowan MM. Plant products as antimicrobial agents. Clin Microbiol

Rev 1999;12:564-82.

Jana S, Shekhawat GS. Phytochemical Analysis and Antibacterial

Screening of in vivo and in vitro Extracts of Indian Medicinal

Herb: Anethum graveolens. Res J Med Plant 2010;4:206-12.

Preethi R, Devanathan VV, Loganathan M. Antimicrobial and

antioxidant efficacy of some medicinal plants against food borne

pathogens. Adv Biol Res 2010;4:122-5.

Maji S, Dandapat P, Ojha D, Maity C, Halder SK, Das PK, et al.

In vitro antimicrobial potentialities of different Solvent extracts

of ethnomedicinal plants against clinically isolated human

pathogens. J Phytology 2010;2:57-64.



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