Extraction of Ashwagandha by conventional extraction methods and evaluation of its anti-stress activity

H. Jain, S. D. Parial, E. Jarald, Anwar S. Daud, Showkat Ahmad

Abstract


The present study was conducted to compare the yield and the antistress activity of Ashwagandha (Withania somnifera) extracts,
using two extraction methods: hot continuous percolation and maceration. Various parameters like temperature, extraction time (10 hours) solvents (water, alcohol, hydroalcohol) and drug-solvent ratios (1:6, 1:8, 1:10) were fixed. The highest yield was found to be 16.96% w/w by maceration process using water (1:8). The activity of different extracts was done by Plus-Maze model using alprazolam as the standard drug. Significant result was found in water extract and hydroalcoholic (1:8) extract prepared by maceration method and also in hydro-alcoholic extract (1:8) prepared by soxhlet process.
Key words: Alprazolam, anti-stress activity, elevated plus maze, extraction, withania somnifera

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References


Jain NK. Sharma SN. A textbook of profesional pharmacy. 4th ed.

Delhi: Vallabh Prakashan; 2003. p. 296-8.

Mukherjee PK. Quality control of herbal drugs. Pharmaceutical

Publishers; 2003. p. 571-3.

Tripathi KD. Essentials of medicinal pharmacology. 5th ed. New

Delhi: 2003. p. 405.

Andallu B, Radhika B. Hypoglycemic diuretic and

hypocholesterolemic effect of winter cherry (Withania somnifera

Dunal) root. Indian J Exp Biol 2000;38:607-9.

Bhatia P, Rattan SIS, Cavallius J, Clark BFC. Withania somnifera

(Ashwagandha) a so-called rejuvenator inhibits growth and

macromolecular synthesis of human cells. Med Sci Res 1987;15:515-6.

Kokate CK, Analytical pharmacognosy. 15th ed. Pune: Nirali

Prakashan; 2000. p. 48.

Khandelwal KR. Practical pharmacognosy: Techniques and

experiment. 16th ed. Nirali Prakashan; 2006. p. 149-54.

Trease and Evans, Pharmacognosy. 15th ed. W.B. Saunders; 2002. p. 46.

OECD Guidelines for testing of chemicals Acute Toxicity-Fixed

Dose Procedure 2004. p. 420.

Kulkarni SK. Handbook of experimental pharmacology. 2nd ed.

New Dehli: Vallabh Prakashan; 1993. p. 5.




DOI: http://dx.doi.org/10.22377/ijgp.v4i3.143

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