Application of high performance liquid chromatography to the determination and validation of levodopa in methanolic extract of Mucuna utilis

Raman M. Singh, Pawan K. Saini, Satish C. Mathur, Gyanendra N. Singh, Santosh Kumar

Abstract


A sensitive, precise and accurate high-performance liquid chromatographic (HPLC) method has been developed for the analysis of levodopa in methanolic extract of Mucuna utilis. The method utilises sample preparation step followed by separation on a Eurosphere C18, 250 × 4.0 mm, 5 μm particle size column, using methanol and 0.5% v/v acetic acid in the ratio of 70:30 v/v as the mobile phase. Analysis of levodopa was carried out in the absorbance mode at 284 nm. The method was validated in terms of linearity, precision (inter and intra day), accuracy, Limit of Detection (LOD) and Limit of Quantitation (LOQ). The proposed HPLC method was found to be precise, specific, accurate and can be used for the identification and quantitative determination of levodopa in herbal extracts.
Key words: High-performance liquid chromatography, levodopa, method development and validation, Mucuna utilis

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References


Modi KP, Patel NM, Goyel RK. Estimation of L-Dopa from Mucuna

pruriens LINN and formulations containing M. pruriens by

HPTLC method. Chem Pharm Bull 2008;56:357-9.

Rajeshwar Y, Gupta M, Mazumadar UK. In vitro lipid peroxidation

and antimicrobial activity of Mucuna pruriens seeds. Iranian J

Pharmacol Ther 2005;4:32-5.

Salau A, Odeleye. Antimicrobial activity of Mucuna pruriens on

selected bacteria. Afr J Biotechnol 2007;6:2091-2.

Sathiyanarayanan L, Arulmozhi S. A comprehensive review of

Mucuna pruriens. Pharmacogn Rev 2007;1:157-62.

Anonymous. The wealth of India. New Delhi: PID, CSIR; 1962. p.

-44.

Anonymous. British Pharmacopoeia. Vol. 1 and 2. London: HMSO

Publication Center; 1980. p. 254, 535, 781.

Anonymous. The United State Pharmacopoeia, XXI, 21st Revision.

Rockville: The US Pharmaceutical Convention Inc.; 1987. p. 585-6.

Baranowska I, Płonka J. Determination of levodopa and

biogenic amines in urine samples using high-performance liquid

chromatography. J Chromatogr Sci 2008;46:30-4.

Chen, Jiyou Z, Honglin Z, Zhide H. Determination of levodopa by

capillary zone electrophoresis using an acidic phosphate buffer and

its application in the analysis of beans. J Food Chem 2005;92:381-6.

Wang J, Zhou Y, Fang YZ. Studies of the rate constant of levodopa

methyl ester hydrolysis by LC. J Chromatographia 2005;62:423-8.

Olsovska J, Novotna J, Flieger M, Spizek J. Assay of tyrosine

hydroxylase based on HPLC separation and quantification of

L-Dopa and L-Tyrosine. J Biomed Chromatogr 2007;21:1252-8.

Wang J, Fang Y. Determination, purity assessment and chiral

separation of levodopa methyl ester in bulk and formulation

pharmaceuticals. J Biomed Chromatogr 2006;20:904-10.

Siddhuraju P, Becker K. Studies on antioxidant activities of mucuna

seed (Mucuna pruriens var utilis) extract and various non-protien

amino acids through in vitro models. J Sci Food Agric 2003;83:

-24.

Validation of Analytical Procedures, Methodology, ICH

harmonized tripartite guidelines, 1996. p. 1.




DOI: http://dx.doi.org/10.22377/ijgp.v4i3.137

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