Determination of bacoside A by HPTLC in Bacopa monnieri extract

Om Prakash, Gyanendra N. Singh, Raman M. Singh, Satish C. Mathur, Meenakshi Bajpai, Saroj Yadav


A simple sensitive HPTLC method developed for the determination of bacoside A in the plant Bacopa monnieri extracts. Th e stationary
phase was precoated silica gel GF254. Th e mobile phase used was dichloromethane: methanol: water (4.5: 1.0: 0.1 v/v/v). Th e plate
was scanned and quantifi ed at 225 nm for bacoside A. Th e method was validated in terms of linearity, accuracy and specifi city. Th e proposed HPTLC method provides a faster and cost eff ective qualitative control for routine analysis of bacoside A in extracts containing Bacopa monnieri saponins.
Key words: Analysis, bacoside A, HPTLC

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Kapoor LD. Hand book of Ayurvedic medicinal plants. Boca Raton:

CRC Press; 1990.

Singh HK, Dhawan BN. Neuropsychopharmacological eff ects of

the Ayurvedic nootropic Bacopa monniera Linn. (Brahmi). Indian J

Pharmacol 1997;29:359-65.

Singh HK, Rastogi RP, Srimal RC, Dhawan BN. Eff ect of bacosides A

and B on avoidance responses in rats. Phytother Res 1998;2:70-4.

Nadkarni KM. The Indian materia medica. Columbia MO: South

Asia Books; 1988.

Russo A, Borrelli F. Bacopa monniera, a reputed nootropic plant:

An overview. Phytomedicine 2005;12:305-17.

Kishore K, Singh M. Eff ect of bacosides, alcoholic extract of Bacopa

monniera Linn. (brahmi), on experimental amnesia in mice. Indian

J Exp Biol 2005;43:640-5.

Anbarasi K, Vani G, Balakrishna K, Desai CS. Creatine kinase

isoenzyme patt erns upon chronic exposure to cigarett e smoke:

Table 1: Validation parameters for quantifi cation of

bacoside A by HPTLC

Parameters Results

Precision (% RSD) < 2%

Linearity 8.4 – 50.4 μg/spot

Limit of Detection 3 μg/spot

Limit of Quantifi cation 9.9 μg/spot

Accuracy 98.39 – 100.40 %

Assay 41.5 % w/w

Protective eff ect of Bacoside A. Vascul Pharmacol 2005;42:57-61.

Deepak M, Amit A. The need for establishing identities of ’bacoside

A and B’, the putative major bioactive saponins of Indian medicinal

plant Bacopa monnieri. Phytomedicine 2004;11:264-8.

Pal R, Sarin JP. Quantitative determination of bacosides by UVspectrophotometry.

Indian J Pharm Sci 1992;54:17-8.

Shrikumar S, Sandip S, Ravi TK, Umamaheswari M. HPTLC

determination and Þ nger printing of bacoside a in Bacopa monnieri

and its formulation. Indian J Pharm Sci 2004;66:132-5.

Pal R, Dwivedi AK, Singh S, Kulshrestha DK. Quantitative

determination of bacoside by HPLC. Indian J Pharm Sci


Gupta AP, Mathur S, Gupta MM, Sushil KW. Eff ect of drying

on the bacoside: A content of the harvested Bacopa monniera

shoots revealed using a new High performance thin layer

chromatography. J Med Aromat Plant Sci 1998;20:1052-5.

Renukappa T, Roos G, Klaiber I, Vogler B, Kraus W. Application

of high-performance liquid chromatography coupled to nuclear

magnetic resonance spectrometry, mass spectrometry and bioassay

for the determination of active saponins from Bacopa monniera

Wett st. J Chromatogr A 1999;847:109-16.

Ganzera M, Gampenrieder J, Pawar RS, Khan IA, Stuppner H.

Separation of the major triterpenoid saponins in Bacopa monnieri

by high-performance liquid chromatography. Anal Chim Acta


Deepak M, Sangli GK, Arun PC, Amit A. Quantitative

determination of the major saponin mixture bacoside A in Bacopa

monnieri by HPLC. Phytochem Anal 2005;16:24-9.

ICH Topic Q2B. Validation of analytical procedures: Methodology.

London: 1996.



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