Free radical scavenging activity of methanolic extract of Luffa cylindrica leaves

Neeraj Kant Sharma, Partap Sangh, Priyanka Priyanka, Keshari K. Jha, Hemant K. Singh, Anil K. Shrivastava

Abstract


Context: Free radicals contribute to more than one hundred disorders in humans including atherosclerosis, hypertension, arthritis, ischemia, gastritis, central nervous system injury, reperfusion injury of many tissues, cancer, Alzheimer’s disease, Parkinsonism, diabetes mellitus and AIDS. There is considerable evidence that antioxidants could help to prevent these diseases because they have the capacity to quench free radicals. Aim: Free radical scavenging activity of methanolic extract of the leaves of Luffa cylindrica (MELC) was evaluated in various in vitro systems. Materials and Methods: The methods were extensively reviewed and free radical scavenging activity was performed by employing various in‑vitro assay methods like DPPH, hydroxyl radical, superoxide and nitric oxide scavenging activities. Statistical Analysis: Results were analyzed statistically using one way analysis of variance (ANOVA) followed by Dunnett’s multiple comparison test and were expressed as mean ± SE of three observations. Values of P < 0.05 were considered
significant. Results: In all the studies, a significant correlation existed between concentrations of the extract and percentage inhibition of free radicals. The preliminary phytochemical screening of MELC indicated the presence of terpenoids, steroids, flavonoids and glycosides. The extract was found to contain 53.78 ± 1.01 μg/mg total polyphenolics expressed as GAE (micrograms per milligram of GAE). Conclusion: The results of the study suggested that the methanolic extract of the leaves of Luffa cylindrica possessed a significant scavenging effect with increasing concentrations probably due to its antioxidant potential and could serve as a potential source of natural antioxidants effective in treatments against free radical mediated diseases.
Key words: Antioxidant, Luffa cylindrica, methanolic extract, phenolic compounds

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DOI: http://dx.doi.org/10.22377/ijgp.v6i3.267

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