Free radical scavenging activity of methanolic extract of Luffa cylindrica leaves

Neeraj Kant Sharma, Partap Sangh, Priyanka Priyanka, Keshari K. Jha, Hemant K. Singh, Anil K. Shrivastava


Context: Free radicals contribute to more than one hundred disorders in humans including atherosclerosis, hypertension, arthritis, ischemia, gastritis, central nervous system injury, reperfusion injury of many tissues, cancer, Alzheimer’s disease, Parkinsonism, diabetes mellitus and AIDS. There is considerable evidence that antioxidants could help to prevent these diseases because they have the capacity to quench free radicals. Aim: Free radical scavenging activity of methanolic extract of the leaves of Luffa cylindrica (MELC) was evaluated in various in vitro systems. Materials and Methods: The methods were extensively reviewed and free radical scavenging activity was performed by employing various in‑vitro assay methods like DPPH, hydroxyl radical, superoxide and nitric oxide scavenging activities. Statistical Analysis: Results were analyzed statistically using one way analysis of variance (ANOVA) followed by Dunnett’s multiple comparison test and were expressed as mean ± SE of three observations. Values of P < 0.05 were considered
significant. Results: In all the studies, a significant correlation existed between concentrations of the extract and percentage inhibition of free radicals. The preliminary phytochemical screening of MELC indicated the presence of terpenoids, steroids, flavonoids and glycosides. The extract was found to contain 53.78 ± 1.01 μg/mg total polyphenolics expressed as GAE (micrograms per milligram of GAE). Conclusion: The results of the study suggested that the methanolic extract of the leaves of Luffa cylindrica possessed a significant scavenging effect with increasing concentrations probably due to its antioxidant potential and could serve as a potential source of natural antioxidants effective in treatments against free radical mediated diseases.
Key words: Antioxidant, Luffa cylindrica, methanolic extract, phenolic compounds

Full Text:



Majarab M, Emami SA, Hasssanzadeh‑Khayyat M. Antioxidant

activity of methanolic extracts of different species of Artemisia

from Irab. Pharmacol Online 2009;2:797‑07.

Aher VD, Wahi AK, Pawdey AM, Sonawane A. Antioxidants as

Immunomodulator: An expanding research avenue. Int J Curr

Pharm Res 2011;3:8‑10.

Naphade SS, Khadabadi SS, Deore SL, Jagtap NS, Hadke SP.

Antioxidant Activity of different extracts of plant Tricholepsi

Glaberrima DC (Asteraceae). Int J Pharm Tech Res 2009;5:502‑5.

Willett WC. The Mediterranean diet: Science and practice. Public

Health Nutrition 2006;9:105‑10.

Chu Y. Flavonoid content of several vegetables and their

antioxidant activity. J Sci Food Agric 2000;80:561‑6.

Yoganandam PG, Ilango K, Kumar S, Elumalai A. In‑vitro

antioxidant activity of L. cylindrica seeds oil. J Global Pharma

Technol 2010;2:93‑7.

Yang Y, Ma X, Wu W, Guo P. Biological characters of the different

varieties for Luffa cylindrica. Zhong Yao Cai 1999;22:165‑7.

Oboh IO, Aluyor EO. Luffa cylindrica‑an emerging cash crop.

African J Agric Res 2009;4: 684‑8.

Nagao T, Lanaka R, Iwase Y, Hanazone H, Okabe H. Studies

on the constituents of Luffa acutangula Roxb. Clin Pharm Bull


Oyetayo FL, Oyetayo VO, Ajewole V. Phytochemical profile and

antibacterial properties of the seeds and leaf of the Luffa plant

(L. cylindrica). J Pharmacol Toxicol 2007;2:586‑9.

Indumathy R, Satheesh DK, Kolagani P, Sashikala GD.

Antimicrobial activity of whole plant of Luffa cylindrica (Linn)

against some common pathogenic micro‑organisms. Int J Pharm

Sci Drug Res 2011;3:29‑31.

Shete RV, Pawashe PM, Kore KJ, Otari KV. Protective role of

Luffa cylindrica linn against erythromycin estolate induced

hepatotoxicity. Curr Pharma Res 2011;1:315‑9.

Chopra RN. Glossary of Indian Medicinal Plants. 1st ed, Vol. 2.

New Delhi, India: Council of Scientific and Industrial Research;

Rehm S, Enslin PR, Meeuse J, Wessels JH. Bitter principles of the

Cucurbitaceae. VII ‑ The distribution of bitter principles in this

plant family. J Sci Agric 1957;8:679‑86.

Harborne JB. Phytochemical Methods: A Guide to Modern

Technique of Plant Analysis. 3rd Edition. New York: Chapman and

Hall; 1984; 1-198.

Liu F, Ng TB. Antioxidative and free radical scavenging activities

of selected medicinal herbs. Life Sci 2000;66:725‑35.

Garrat DC. The Quantitative Analysis of Drugs. Japan: Chapman

and Hall; 1964; 17-120.

Zeyep T, Muberra K, Esra K. Antioxidant, anti‑inflammatory,

anti‑nociceptive activities and composition of Lythrum salicaria L.

extracts. J Ethnopharmacol 2007;110:539‑47.

Halliwell B, Gutteridge J. Formation of a thiobarbituric‑acid‑reactive

substance from deoxyribose in the presence of iron salts. The role

of superoxide and hydroxyl radicals. FEBS Lett 1981;128:347‑52.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal

tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351‑8.

Sroks Z, Cisowski W. Hydrogen peroxide scavenging, antioxidant

and antiradical activity of some phenolic acids. Food Chem Toxicol


Oyaizu M. Studies on products of browning reactions:

Antioxidative activities of products of browning reaction prepared

from glucosamine. JPN J Nutr 1996;44:307‑15.

Singleton VL, Orthofer RM, Ramuela‑Raventos RM. Analysis of total

phenols and other oxidation substrates and antioxidants by means

of folin‑ciocalteu reagent. Meth Enzymol 1999;299:152‑78.

Gulcin L, Alici HA, Cesur M. Determination of in vitro antioxidant

and radical scavenging activities of propofol. Chem Pharm Bull


Gulcin I, Oktay M, Kirecci E, Kufrevioglu OI. Screening of

antioxidant and Antimicrobial activities of anise (Pimpinella

anisum L) seed extracts. Food Chem 2003;83:371‑82.

Ialenti A, Moncada S, Di Rosa M. Modulation of adjuvant arthritis

by endogenous nitric oxide. Br J Pharmacol 1993;110:701‑05.

Wang M, Li J, Rangarajan M, Shao Y, La Voie EJ, Huang T, et al.

Antioxidative phenolic compounds from sage (Salvia officinalis).

J Agric Food Chem 1998;46:4869‑73.

Aruoma OI. Free radicals, antioxidants and international nutrition.

Asia Pacific J Clin Nutri 1999;8:53‑63.

Yen GC, Duh PD. Scavenging effect of methanolic extracts of

peanut hulls on free‑radical and active‑oxygen species. J Agri Food

Chem 1994;42:629‑32.



  • There are currently no refbacks.