Omega-3 fatty acids improve psychomotor performance via mechanism not related to nitric acid production

Marwan S. M. Al-Nimer, Ali I. Al-Gareeb, Hayder M. Al-Kuraishy


Omega-3 fatty acids (ω-3FAs) are essential polyunsaturated fats that protect the brain from cognitive impairment. It increases the
activity of endothelial nitric oxide synthetase (eNOS) and thereby increases the nitric acid (NO) production. This study aimed to
explore the effect of ω-3FAs on psychomotor performance and to relate this effect to the reactive nitrogen species. This study was
conducted in Department of Pharmacology, College of Medicine, Al-Mustansiriya University in Baghdad, Iraq. Twenty healthy subjects,
allocated randomly from medical college students, were participated in the single blind clinical trial. Participants were divided into
two groups, each of ten subjects to receive either placebo or (ω-3FAs) (750 mg single oral dose daily for 5 days). They were asked
to perform psychomotor performance before and after 5 days of treatment, and venous blood was obtained for determination of
serum nitric oxide (NO) and peroxynitrite (ONOO). ω-3FAs treated group was significantly different from placebo-treated group in reducing choice and motor reaction times as well as the critical flicker frequency threshold. The serum levels of NO and ONOO in ω-3FAs-treated group did not significantly differ from placebo-treated group. Short term supplementation of ω-3FAs improves the psychomotor performance in young healthy subjects via a mechanism not related to the production of nitric oxide production. Inflorescence is a panicle few flowered and fruit is a capsule. The data of the results obtained were presented and discussed.
Key words: Nitrogen species, psychomotor performance, ω-3FAs

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Yehuda S, Rabinovitz S, Carasso RL, Mostofsky DI. The role

of polyunsaturated fatty acids in restoring the aging neuronal

membrane. Neurobiol Aging 2002;23:843-53.

McCann JC, Ames BN. Is docosahexaenoic acid, an n-3 longchain

polyunsaturated fatty acid, required for development of

normal brain function? An overview of evidence from cognitive

and behavioral tests in humans and animals. Am J Clin Nutr


Chang CY, Ke DS, Chen JY. Essential fatty acids and human brain.

Acta Neurol Taiwan 2009;18:231-41.

Dyall SC, Michael GJ, Michael-Titus AT. Omega-3 fatty acids

reverse age-related decreases in nuclear receptors and increase

neurogenesis in old rats. J Neurosci Res 2010;88:2091-102.

Ikemoto A, Ohishi M, Sato Y, Hata N, Misawa Y, Fujii Y, et al.

Reversibility of n-3 fatty acid deficiency-induced alterations of

learning behavior in the rat: level of n-6 fatty acids as another

critical factor. J Lipid Res 2001;42:1655-63.

Calon F, Lim GP, Yang F, Morihara T, Teter B, Ubeda O, et al.

Docosahexaenoic acid protects from dendritic pathology in an

Alzheimer's disease mouse model. Neuron 2004;43:633-45.

Hashimoto M, Hossain S, Shimada T, Sugioka K, Yamasaki H,

Fujii Y, et al. Docosahexaenoic acid provides protection from

impairment of learning ability in Alzheimer's disease model rats.

J Neurochem 2002;81:1084-91.

Hashimoto M, Hossain S, Agdul H, Shido O. Docosahexaenoic

acid-induced amelioration on impairment of memory learning in

amyloid beta-infused rats relates to the decreases of amyloid beta

and cholesterol levels in detergent-insoluble membrane fractions.

Biochim Biophys Acta 2005;1738:91-8.

Green KN, Martinez-Coria H, Khashwji H, Hall EB, Yurko-

Mauro KA, Ellis L, et al. Dietary docosahexaenoic acid and

docosapentaenoic acid ameliorate amyloid-beta and tau pathology

via a mechanism involving presenilin 1 levels. J Neurosci


McNamara RK, Able J, Jandacek R, Rider T, Tso P, Eliassen JC, et al.

Docosahexaenoic acid supplementation increases prefrontal cortex

activation during sustained attention in healthy boys: A placebocontrolled,

dose-ranging, functional magnetic resonance imaging

study. Am J Clin Nutr 2010;91:1060-7.

López D, Orta X, Casós K, Sáiz MP, Puig-Parellada P, Farriol M, et al.

Upregulation of endothelial nitric oxide synthase in rat aorta after

ingestion of fish oil-rich diet. Am J Physiol Heart Circ Physiol


Morgan DR, Dixon LJ, Hanratty CG, El-Sherbeeny N, Hamilton

PB, McGrath LT, et al. Effects of dietary omega-3 fatty acid

supplementation on endothelium-dependent vasodilation in

patients with chronic heart failure. Am J Cardiol 2006;97:547-51.

Filaire E, Massart A, Portier H, Rouveix M, Rosado F, Bage AS, et al.

Effect of 6 Weeks of n-3 fatty-acid supplementation on oxidative

stress in Judo athletes. Int J Sport Nutr Exerc Metab 2010;20:


Matesanz N, Park G, McAllister H, Leahey W, Devine A, McVeigh

GE, et al. Docosahexaenoic acid improves the nitroso-redox balance

and reduces VEGF mediated angiogenic signaling in microvascular

endothelial cells. Invest Ophthalmol Vis Sci 2010;51:6815-25.

Beckman JS, Ischiropoulos H, Zhu L, van der Woerd M, Smith

C, Chen J. Kinetics of superoxide dismutase and iron -catalyzed

nitration of phenolics by peroxynitrite. Arch Biochem Biophys


VanUffelen BE, Van Der Zee J, De Koster BM, Vanstereninck

J, Elferink JG. Intracellular but not extracellular conversion of

nitroxyl anion into nitric oxide leads to stimulation of human

neutrophil migration. Biochem J 1998;330:719-22.

Newaz MA, Yousefipour Z, Nawal N, Adeeb N. Nitric oxide

synthase activity in blood vessels of spontaneously hypertensive

rats: Antioxidant protection by gamma-tocotrienol. J Physiol

Pharmacol 2003;54:319-27

Fontani G, Corradeschi F, Felici A, Alfatti F, Migliorini S, Lodi L.

Cognitive and physiological effects of Omega-3 polyunsaturated

fatty acid supplementation in healthy subjects. Eur J Clin Invest


Ferraz AC, Delattre AM, Almendra RG, Sonagli M, Borges C,

Araujo P, et al. Chronic ω-3 fatty acids supplementation promotes

beneficial effects on anxiety; cognitive and depressive-like

behaviors in rats subjected to a restraint stress protocol. Behav

Brain Res 2011;219:116-22

Avraham Y, Saidian M, Burston JJ, Mevorach R, Vorobiev L, Magen I,

et al. Fish oil promotes survival and protects against cognitive

decline in severely undernourished mice by normalizing satiety

signals. J Nutr Biochem 2011;22:766-76

Quinn JF, Raman R, Thomas RG, Yurko-Mauro K, Nelson EB,

Van Dyck C, et al. Docosahexaenoic acid supplementation and

cognitive decline in Alzheimer disease: A randomized trial. JAMA


Hindmarch I. Psychomotor function and psychoactive drugs. Br

J Clin Pharmacol 1980;10:189-209.

Ambrozova G, Pekarova M, Lojek A. Effect of polyunsaturated

fatty acids on the reactive oxygen and nitrogen species production

by raw 264.7 macrophages. Eur J Nutr 2010;49:133-9.



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