Quantitative estimation of silybin in Silybum marianum mother tincture using high performance liquid chromatography

Purnima D. Hamrapurkar, Leena V. Thakurdesai, Mitesh D. Phale


Silybin is a characterising compound present in the plant Silybum marianum, used as an hepatoprotective. A simple, accurate and
sensitive HPLC method was developed for estimation of silybin. The chromatographic separation was carried out on RP C18 (Waters)
column (250 × 4.6 mm, 10 μm) using mobile phase, phosphate buffer: water (65:35 % v/v) pumped at a flow rate of 1 ml/min. The
eluent was monitored at 288 nm. The method is specific and linear over the range of 10-200 ng/ml. The method was statistically
validated for precision, accuracy, robustness and recovery. The HPLC method can be applied for identification and on of silybin in
herbal extracts of Silybum marianum.
Key words: HPLC, mother tincture, quantitative estimation, silybin, Silybum marianum

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DOI: http://dx.doi.org/10.22377/ijgp.v4i4.153


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