Phenanthrenes of Eulophia ochreata Lindl

Rajendra D. Kshirsagar, Yogesh B. Kanekar, Suresh D. Jagtap, Shakti N. Upadhyay, Rajesh Rao, Sandip P. Bhujbal, Jagdish N. Kedia


Pawra tribe of Satpuda mountain ranges that belong to Maharashtra region, India, is known to use tubers of Eulophia ochreata for rejuvenating and aphrodisiac properties and also for curing rheumatism. For systematic scientific validation of folk claims of E. ochreata tubers, we report here the collection, extraction, isolation and spectral characterization of molecules and also its evaluation for free radical scavenging activity. Isolation of molecules was carried out on activity-guided fractionation in 2,2’-diphenyl-1-picrylhydrazyl assay. This is the first report of isolation of 9,10-Dihydro 2,5-Dimethoxyphenanthrene-1,7-diol and 5,7-Dimethoxyphenanthrene-
2,6-diol from this orchid.
Key words: Antioxidant activity; DPPH; Eulophia ochreata; tubers; 9,10-Dihydro-2,5-Dimethoxyphenanthrene-1,7-diol;
5,7-Dimethoxyphenanthrene-2,6-diol; phenanthrenes

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Tominaga H, Kobayashi Y, Goto T, Kasemura, Momura M. DPPH

Radical-scavenging effect of several phenylpropanoid compounds

and their glycoside derivatives. Yakugaku Zasshi 2005;125:371-5.

Takagi S, Yamaki M, Inoue K. Antimicrobial agents from Bletilla

striata. Phytochemistry 1983;22:1011-5.

Majumder PL, Kar A, Shooler JN. Bulbophyllanthrin, a

phenanthrene of the orchid Bulbophyllum leopardium.

Phytochemistry 1985;24:2083-7.

Majumder P, Bandyopadhyay D, Joardar S. Coelogin and

Coeloginin: Two novel 9, 10 Dihydrophenanthrene derivatives

from the orchid Coelogyne cristata. J Chem Soc Perkin Trans


Majumder PL, Laha S, Datta N. Coelonin, a 9, 10-

Dihydrophenanthrene from the orchids Coelogyne ochracea and

Coelogyne elata. Phytochemistry 1982;21:478-80.

Majumder PL, Laha S. Occurrence of 2, 7-Dihydroxy-3, 4,

-trimethoxy-9, 10-Dihydrophenanthrene in Coelogyne ovalis,

a high altitude Himalayan Orchid: Application of C-13 NMR

spectroscopy in structure elucidation. J Indian Chem Soc


Sachdev K, Kulshreshtha DK. Phenolic constituents of Coelogyne

ovalis. Phytochemistry 1986;25:499.

Rajaraman K, Rangaswami S. Structure of two new 9,

-Dihydrophenanthrene from Dioscorea prazeri. Indian J Chem


Bhandari SR, Kapadi AH, Majumder PL, Joardar M, Shoolery JN.Nudol, a phenanthrene of the orchids Eulophia nuda, Eria carinata

and Eria stricta. Phytochemsitry 1985;24:801-4.

M a j u m d e r P L , J o a r d a r M . E r i a n t h r i d i n , a n e w 9 ,

-Dihydrophenanthrene derivative from the orchids Eria carinata

and Eria stricta. Indian J Chem Br 1985;24:1192-4.

Majumder PL, Kar A. Confusarin and Confusaridin, two

phenanthrene derivatives of the orchid Eria confusa.

Phytochemistry 1987;26:1127-9.

Bhandari SR, Kapadi AH. A 9, 10-Dihydrophenanthrene from

tubers of Eulophia nuda. Phytochemsitry 1983;22:747-8.

Tuchinda Patoomratana, Jinda U, Kanjai K, Walter C. Benzylated

phenanthrenes from Eulophia nuda. Phytochemistry 1989;27:3267-71.

Tuchinda P, Jinda U, Kanjai K, Walter C, Lutz M, Allan H.

Phenanthrenes for Eulophia nuda. Phytochemistry 1988;27:3267-71.

Blitzke T, Masaoud M, Schimidt J. Constituents of Eulophia

petersii. Fitoterapia 2000;71:593-4.

Fisch MH, Flick BH, Arditti J. Structure and antifungal activity of

Hircinol, Loroglossol and Orchinol. Phytochemistry 1973;12:437.

Samuel E, Ruben AT, Rachel M. New phenanthrene derivatives

from Maxillaria densa. J Nat Prod 1999;62:1175-8.

Majumder P, Sarkar A, Chakraborti J. Isoflavidinin and isooxoflavidinin,

two 9, 10-Dihydrophenanthrenes from the orchids

Pholidota articulata, Otochilus protecta and Otochilus fusca.

Phytochemistry 1982;21:2713-6.

Wang J, Wang L, Kitanaka S. Stilbene and Dihydrophenanthrene

derivatives from Pholidota chinensis and their nitric oxide

inhibitory and radical-scavenging activities. J Natural Med


Lauoshan, Zheng WT. Yannan phenolic constituents of Pholidota.

Chinese Pharmaceut J 2005;40:255-8.

Stewart J. Orchids of Kenya. Portland, Oregon, USA: Timber Press

Inc.; 1996.

Thomas SA. A preliminary checklist of the genus Eulophia.

Lindleyana 1998;13:170-202.

Bhattacharjee SK. Indian Eulophias. Am Orchid Soc Bull


Shetty BV, Singh V. Flora of Rajasthan Botanical Survey of India.

Vol. 2. Calcutta. India: 1991.

Khanna KK, Mudgal V, Kumar A. Three orchids new to Madhya

Pradesh. J Economica Taxonomic Botany 1999;23:711-2.

Reddy SC, Pattanaik C, Murthy MS, Reddy KN. Orchids of Eastern

Ghats, India. EPTRI-ENVIS New Letter 2005;11:6-12.

Cooke T. The flora of the presidency of Bombay, Vol. 3. (BSI, Reprint

. Calcutta; 1958.

Santapau H, Kapadia Z. The Orchids of Bombay. Delhi:

Government of India Press; 1966.

Sharma BD, Karthikeyan S, Singh NP. Flora of Maharashtra state

Monocotyledons. Botanical Survey of India, Calcutta, India; 1996.

Philip M. The use of the stable free radical diphenylpicrylhydrazyl

(DPPH) for estimating antioxidant activity. Songkanakarin. J Sci

Tech 2004;26:211-9.

Katalinic V, Milos M, Kulisic T, Jukic M. Screening of 70 medicinal

plant extracts for antioxidant capacity and total phenols. Food

Chem 2006;94:550-7.



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