In vitro antibacterial activity of Artemisia annua Linn. growing in India

Prakash C. Gupta, B. Dutta, D. Pant, P. Joshi, D. R. Lohar


The crude extracts obtained from the aerial parts of Artemisia annua Linn. (Asteraceae) were investigated for their antibacterial activity by using agar well diffusion assays against five Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis, Bacillus pumilus, Bacillus cereus, and Micrococcus luteus) and three Gram-negative bacteria (Escherichia coli, Salmonella typhi, and Pseudomonas aeruginosa). Of the various extracts, the methanol extract showed the strongest activity against most bacteria used in this study. The most sensitive organism to the extracts was M. luteus. The minimum inhibitory concentration (MIC) values were determined by the tube dilution method. The results showed that S. aureus required ~0.25 mg/mL of the methanol extract for inhibition. The HPTLC
fingerprint of the methanol extract after derivatization with anisaldehyde sulphuric acid reagent showed a maximum number of separated components. TLC bioautography of the methanol extract showed that the area of inhibition around compounds differentiated at Rf = 0.32, Rf = 0.42, Rf = 0.46, Rf = 0.77, and Rf = 0.87 against S. aureus. This is the first report of the antibacterial activity of A. annua against food-borne bacteria. The results indicated that aerial parts of A. annua might be potential sources of new antibacterial agents.
Key words: Antibacterial activity, Artemisia annua, MIC, TLC bioautography

Full Text:



Santos PR, Oliveira AC, Tomassini TC. Controle microbiogico de

produtos fitoterapicos. Rev Farm Bioquim 1985;31:35-8.

Farnsworth NR, Soejarto DD. Potential consequence of plant

extinction in the United States on the current and future availability

of prescription drugs. Econ Bot 1985;39: 231-40.

Cerdeiras MP, Albores S, Etchcverry S, Lucian V, Soubes M,

Vazquez A. Antimicrobial activity of Xanthium cavanillesii

extracts. Pharm Biol 2007;45:251-4.

Recio MC, Rios JL, Villar A. A review of some antimicrobial

compounds isolated from medicinal plants reports in literature.

Phytother Res 1989;3:117-25.

Essawi T, Srour M. Screening of some Palestinian medicinal plants

for antibacterial activity. J Ethnopharmacol 2000;70:343-9.

Bhakuni RS, Jain DC, Sharma RP, Kumar S. Secondary metabolites

of Artemisia annua and their biological activity. Curr Sci


Quality Standards of Indian Medicinal Plants. New Delhi: Indian

Council of Medical Research Publication; 2002. p. 21-6.

Mahida Y, Mohan JS. Screening of Indian plant extracts for

antibacterial activity. Pharm Biol 2006;44:627-31.

Perez C, Pauli M, Bazerque P. An antibiotic assay by the agar-well

diffusion method. Acta Biol Med Exp 1990;15:113-5.

NCCLS (National Committee for Clinical Laboratory Standards),

Approved standard M7-A5. Standard methods for dilution

antimicrobial susceptibility test for bacteria that grow aerobically.

Villanova PA 1990.

Juteau F, Masotti V, Bessière JM, Dherbomez M, Viano J.

Antibacterial and antioxidant activities of Artemisia annua

essential oil. Fitoterapia 2002;73:532-5.

Nair R, Kalariya T, Chanda Sumitra. Antibacterial activity of some

Indian medicinal flora. Terk J Biol 2005;29:41-7.

Parkeh J, Chanda S. Screening of aqueous and alcoholic extracts

of some Indian medicinal plats for antibacterial activity. Indian J

Pharma Sci 2006;68:835-8.

Encarnacion Dimayuga R, Virgen M, Ochoa N. Antimicrobial

activity of medicinal plants from Baja California Sur (Mexico).

Pharm Biol 1998;36:33-4.



  • There are currently no refbacks.