Modulatory effect of an isolated compound from Syzygium cumini seeds on biochemical parameters of diabetes in rats

Mamta Farswan, Papiya Mitra Mazumder, V. Parcha


In the search of natural hypoglycemic agents as alternatives to synthetic ones and to justify the use of Syzygium cumini seeds in
folklore system of medicine for diabetes the present study was carried out. To evaluate the hypoglycemic and antioxidant activity of an isolated compound from S. cumini seeds in normal and non-insulin dependent diabetes mellitus (NIDDM) rats. Study was carried out in Wistar rats. Diabetes was induced by streptozotocin in neonates. Oral administration of petroleum ether, chloroform, acetone,methanol, and water extracts of S. cumini (100 mg/kg, p.o.) for 21 days caused a decrease in fasting blood sugar (FBS) in diabetic rats. Among all the extracts, methanol extract was found to lower the FBS significantly in diabetic rats. Glibenclamide was used as standard antidiabetic drug (5 mg/kg, p.o). Methanol extract was subjected to column chromatography that led to isolation of an active principle, which was given trivial name Cuminoside. Cuminoside (50 mg/kg, p.o.) was studied for its hypoglycemic and antioxidant
potential. The unpaired t-test and analysis of variance (ANOVA) followed by post hoc test were used for statistical analysis. Cuminoside caused a significant decrease in FBS level, lipidperoxidation level, and improvement in the levels of antioxidant enzymes (reduced glutathione, superoxide dismutase, and catalase) in diabetic rats. A considerable decrease in lipid peroxidation and improvement
in the antioxidant enzymes level in NIDDM rats indicated that Cuminoside has antioxidant potential with antidiabetic activity and provides a scientific rationale for the use of Cuminoside as an antidiabetic agent.
Key words: Antioxidants, β sitosterol, diabetes, lipid peroxidation, streptozotocin, Syzygium cumini

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