Acute and sub‑chronic toxicity study of Brahmi ghrita in rodents

Yadav Kapil Deo, K. R. C. Reddy, Kumar Vikas

Abstract


Context: Brahmi Ghrita (BG) contains Brahmi (Bacopa monneri), Vacha (Acorus calamus), Kushtha (Saussurea lappa),
Shankhapushpi (Convolvulus pluricaulis) and Puran Ghrita, mentioned for treatment of various diseases. Aim: To assess acute and sub‑chronic toxicity of Brahmi Ghrita in mice and rats, respectively. Materials and Methods: In acute toxicity study, Swiss strain albino mice were administered orally Brahmi Ghrita doses of 1, 2.5 and 5 g/kg and observed for behavioural changes and mortality, if any. In sub chronic toxicity study, Charles Foster albino rats were administered two doses of Brahmi Ghrita i.e., 400 and 800 mg/kg, p.o. for 30 consecutive days. During 30 days of treatment, rats were observed for any changes in body weight and daily food and water intake. After 30 days, rats were sacrificed for haematological, biochemical and histopathology study. Result: There was no mortality or
abnormal behaviour, observed in acute toxicity study in mice at all the three dose levels. In sub‑chronic toxicity study, Brahmi Ghrita did not produce any significant changes in body weight and daily food and water intake of rats when compared to control group rats. Further, haematological and biochemical parameters were also found normal. Histopathological study revealed normal architecture of kidney and liver of Brahmi ghrita treated rats. Conclusion: Brahmi Ghrita is safe in rodent and mice.
Key words: Acute toxicity, Brahmi ghrita, sub chronic toxicity

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References


WHO guidelines on safety monitoring of herbal medicines in

pharmacovigilance systems. World Health Organization Geneva;

Saad B, Azaizeh H, Abu‑Hijleh G, Said O. Safety of traditional

arab herbal medicine. Evid Based Complement Alternat Med

;3:433‑9.

Schoepfer AM, Engel A, Fattinger K, Marbet UA, Criblez D,

Reichen J, et al. Herbal does not mean innocuous: Ten cases of

severe hepatotoxicity associated with dietary supplements from

Herbalife products. J Hepatol 2007;47:521‑6.

Singh HK, Dhawan BN. Effect of Bacopa monniera Linn. (Brahmi)

extract on avoidance responses in rat. J Ethnopharmacol

;5:205‑14.

Roodenrys S, Booth D, Bulzomi S, Phipps A, Micallef C, Smoker J.

Chronic effect of Brahmi (Bacopa monneri) on human memory.

Neuropsychopharmacology 2002;27:279‑81.

Singh A, Singh SK. Evaluation of anti fertility potential of Brahmi

in male mouse Contraception 2009;79:71‑9.

Khare CP. Indian Medicinal Plants: An Illustrated Dictionary.

New York: Springer‑Verlag Berlin/Heidelberg 2007.

Gopalakrishna HN, Sudhakar P, Shilin G, Ashok KS, Holla GKS,

Vinod N, et al. Effect of Acorus calamus on Electrical and Chemical

Induced Seizures in mice. IJABPT 2010;2:465‑73.

Shukla DJ, Vyas HA, Vyas MK, Ashok BK, Ravishankar B.

A comparative study on chronic administration of Go Ghrita (cow

ghee) and avik Ghrita (eve ghee) in albimo rats AYU 2012;33:435‑40.

Srivastava S. Sharangadhar Samhita with Jiwanprada Hindi

commentary. Varanasi: Chaukhambha Orientalia Publication; 2009.

Gubbannavar JS, Chandola HM, Harisha CR, Kalyani R Shukla VJ.

Analytical profile of Brahmi Ghrita: A poly herbal Ayurvedic

formulation AYU 2012;33:289‑93.

Singh GK, Kumar V. Acute and sub‑chronic toxicity study

of standardized extract of Fumaria Indica in rodents.

J Ethnopharmacol 2011;134:992‑5.

Sim KS, Nurestri AM, Sinniah SK, Kim KH, Norhanom AW. Acute

oral toxicity of Pereskia bleo and Pereskia grandiofolia in mice.

Phcog Mag 2010;6:67‑70.

Singh A, Dubey SD, Patne S, Kumar V. Acute and sub‑chronic

toxicity study of calcium based ayurvedic ‘Bhasmas’ and a ‘Pishti’

prepared from marine‑sourced animals. J Herb Med Toxicol

;4:35‑47.

Morgan A, Stevens J. Does Bacopa monneri improve memory

performance in older persons? Results of a randomized,

placebo‑controlled, double‑blind trial. J Altern Complement

Med 2010;16:753‑9.

Matsuda H, Kaeura T, Inoue Y. Morikawa T, Yoshikawa M. Absolute

Stereo structures and synthesis of Saussure amines A, B, C, D and E

amino acid –seaquiterpene conjugates with Gastroprotective effect

from the roots of Saussurea lappa. Tetrahedan 2000;56:7763‑77.

Yoshikawa M, Hatakeyama S, Inoue Y, Yamahara J.

Saussureamines A, B, C, D and E, new antiulcer principles from

Chinese Saussurea radix. Chem Pharm Bull 1993;41:214‑6.

Sairam K, Rao CV, Goel RK. Effect of Convolvulus pluricaulis

Chois on gastric ulceration and secretion in rats. Indian J Exp Bio

;39:350‑4.

Chao JY, Baik KV, Jung JH, Park MH. In vitro anti inflammatory

effects of Cynaropicrin, a sesquiterpene lactone from Saussurea

lappa. Eu J Pharmacol 2000;398:399‑407.

The Ayurvedic Pharmacopoeia of India Part I. 1st ed., Vol. I.

Department of AYUSH, Ministry of Health and Family Welfare,

Govt. of India, 1999.

Wong JM, de Souza R, Kendall CW, Emam A, Jenkins DJ.

Colonic health: Fermentation and short chain fatty acids. J Clin

Gastroenterol 2006;40:235‑43.

Rabassa AA, Rogers AI. The role of short‑chain fatty acid

metabolism in colonic disorders. Am J Gastroenterol

;87:419‑23.

Ewers B, Riserus U, Marckmann P. Effects of unsaturated

fat dietary supplements on blood lipids, and on markers of

malnutrition and inflammation in hemodialysis patients. J Ren

Nutr 2009;19:401‑11.

Babalola TO, Apata DF. Chemical and quality evaluation of some

alternative lipid sources for aqua feed production agriculture and

biology. J North Am 2011;2:935‑43.

Yadav KD, Reddy KR, Kumar V. Study of Brahmi Ghrita and

Piracetam in amnesia. Ancient Sci life 2012;32:11‑5.

Olson H, Betton G, Robinson D, Thomas K, Monro A, Kolaja G,

et al. Concordance of toxicity of pharmaceuticals in humans and

in animals. Regul Toxicol Pharmacol 2000;32:56-67.

Mukinda JT, Eagles PF. Acute and sub‑chronic oral toxicity profiles

of the aqueous extract of Polygala fruticosa in female mice and

rats. J Ethnopharmacol 2010;128:236‑40.

Sumathy T, Subramanian S, Govindasamy S, Balkrishna K,

Veluchamy G. Protective role of Bacopa monnieria on morphine

induced hepatotoxicity in rats. Phytother Res 2001;15:643‑5.

Palani S, Raja S, Kumar RP, Venkadesan D, Devi K, Sivaraj A,

et al. Therapeutic efficacy of Anti hepatotoxic and anti antioxidant

Acorus calamus activities of on acetaminophen induced toxicity in

rat. Int J Int Biol 2009;7:39‑44.

Chen HC, Chou CK, Lee SD, Wang JC, Yeh SF. Active compounds

from Saussurea lappa Clarks that suppress hepatitis B virus surface

antigen gene expression in human hepatoma cells. Antiviral Res

;27:99‑109.

Sumathi T, Niranjali Devaraj S. Effect of Bacopa monniera on liver

and kidney toxicity in chronic use of opioids. Phytomedicine

;16:807‑903.

Patel DV, Chandola HM, Baghel MS, Joshi JR. Clinical efficacy of

Shankhapushpi and a herbomineral compound in type II diabetes.

AYU 2012;33:230‑7.

Savitha D Bhat, Ashok BK, Acharya R, Ravishankar B. A comparative

acute toxic evaluation of raw and classically processed rhizome of

Vacha. IJNPR 2012;3:506‑11.

Mishra D, Sinha M, Singh PN, Kumar V. Acute and sub‑chronic

toxicity studies of ayurvedic medicine Garbhpal Ras. E J Pharmacol

Therapy 2008;1:31‑4.




DOI: http://dx.doi.org/10.22377/ijgp.v8i1.349

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