Antioxidant properties of some selected Indian medicinal plants: Their correlation with total phenolic contents

Rayasandra Umesh Abhishek, Devihalli Chikkaiah Mohana, Sreerangegowda Thippeswamy, Kiragandur Manjunath


Background: Antioxidants play a major role in protecting the biological systems against oxidative stress, which is associated with development of many chronic diseases and disorders. Plants are the main sources of natural antioxidants in the form of phenolic compounds, which help human beings to deal with oxidative stress, caused by free radical damage. Aim: The present study aimed at the evaluation of antioxidant properties of extracts of different medicinal plants and their correlation with the total phenolic contents (TPCs). Materials and Methods: The selected plant materials were sequentially extracted with solvents with increasing polarity and analysed for their phytochemical constituents and antioxidant properties. The free radical scavenging activity of solvent extracts was assessed by 2,2‑diphenyl‑1‑picrylhydrazyl assay and TPCs by Folin‑Ciocalteu method. Results and Conclusions: In preliminary assay, 35 plants were screened for antioxidant properties, among them four plants were selected for the study. The methanol
extracts of Acacia catechu (AC‑ME), Adenanthera pavonia (AP‑ME), Holopetelea integrifolia (HI‑ME) and ethanol extract of Terminalia paniculata (TP‑EE) showed highest antioxidant activity with 50% inhibitory concentration (IC50) value ranging from 7.5 to 350 µg/ mL. The percentage radical scavenging activities of samples were compared to the standard butylated hydroxytoluene (BHT). The antioxidant activity of extracts and BHT in increasing order was HI‑ME < AP‑ME < TP‑EE < BHT < AC‑ME. A positive correlation was observed between antioxidant activity and TPCs. The results suggested that the phenolic compounds in extracts are most likely to be responsible for the observed antioxidant activity.
Key words: Antioxidant activity, correlativity, 2,2‑diphenyl‑1‑picrylhydrazyl assay, medicinal plants, phytochemicals, total phenolic contents

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Zeng LB, Zhang ZR, Luo ZH, Zhu JX. Antioxidant activity and

chemical constituents of essential oil and extracts of Rhizoma

Homalomenae. Food Chem 2011;125:456‑3.

Bamoniri A, Ebrahimabadi AH, Mazoochi A, Behpour M, Kashi FJ,

Batooli H. Antioxidant and antimicrobial activity evaluation and

essential oil analysis of Semenovia tragioides Boiss. from Iran. Food

Chem 2010;122:553‑8.

Trigui M, Hsouna AB, Tounsi S, Jaoua S. Chemical composition and

evaluation of antioxidant and antimicrobial activities of Tunisian

Thymelaea hirsuta with special reference to its mode of action. Ind

Crops Prod 2013;41:150‑7.

Ebrahimabadi AH, Ebrahimabadi EH, Djafari‑Bidgoli Z, Kashi FJ,

Mazoochi A, Batooli H. Composition and antioxidant and

antimicrobial activity of the essential oil and extracts of Stachys

inflata Benth from Iran. Food Chem 2010;119:452‑8.

Tippani R, Porika M, Allenki V, Anreddy RN, Yellu NR, Krishna DR,

et al. Antioxidant and analgesic activities of Pterocarpus marsupium

Roxb. J Herbs Spices Med Plants 2010;16:63‑8.

Priya OS, Viswanathan MB, Balakrishna K, Venkatesan M.

Chemical constituents and in vitro antioxidant activity of

Phyllanthus wightianus. Nat Prod Res 2011;25:949‑58.

Aiyegoro OA, Okoh AI. Preliminary phytochemical screening and

in vitro antioxidant activities of the aqueous extract of Helichrysum

longifolium DC. BMC Complement Altern Med 2010;10:21.

Thippeswamy S, Praveen P, Mohana DC, Manjunath K.

Antimicrobial evaluation and phytochemical analysis of a known

medicinal plant Samanea saman (Jacq) Merr against some human

and plant pathogenic bacteria and fungi. Int J Pharm Bio Sci


Dureja AG, Dhiman K. Free radical scavenging potential and total

phenolic and flavonoids content of Ziziphus mauritiana and Ziziphus

nummularia fruit extracts. Int J Green Pharm 2012;6:187‑92.

Mohana DC, Prasad P, Vijaykumar V, Raveesha KA. Plant extract

effect on seed‑borne pathogenic fungi from seeds of paddy grown

in Southern India. J Plant Prot Res 2011;5:101‑6.

Sofowara A. Medicinal Plants and Traditional Medicine in Africa.

Ibadan, Nigeria: Spectrum Books Ltd.; 1993. p. 289‑91.

Rojas R, Bustamante B, Bauer J, Fernández I, Albán J, Lock O.

Antimicrobial activity of selected Peruvian medicinal plants.

J Ethnopharmacol 2003;88:199‑204.

Li HB, Wong CC, Cheng KW, Chen F. Antioxidant properties

in vitro and total phenolic contents in methanol extracts from

medicinal plants. LWT‑Food Sci Technol 2008;41:385‑90.

Ebrahimabadi AH, Mazoochi A, Kashi FJ, Djafari‑Bidgoli Z,

Batooli H. Essential oil composition and antioxidant

and antimicrobial properties of the aerial parts of Salvia

eremophila Boiss. from Iran. Food Chem Toxicol 2010;


Esmaeili MA, Sonboli A. Antioxidant, free radical scavenging

activities of Salvia brachyantha and its protective effect against

oxidative cardiac cell injury. Food Chem Toxicol 2010;48:


Kilani‑Jaziri S, Bhouri W, Skandrani I, Limem I, Chekir‑Ghedira L,

Ghedira K. Phytochemical, antimicrobial, antioxidant and

antigenotoxic potentials of Cyperus rotundus extracts. S Afr J Bot


Castilho PC, Savluchinske‑Feio S, Weinhold TS, Gouveia SC.

Evaluation of the antimicrobial and antioxidant activities of

essential oils, extracts and their main components from oregano

from Madeira Island, Portugal. Food Control 2012;23:552‑8.

Lim YY, Quah EP. Antioxidant properties of different cultivars of

Portulaca oleracea. Food Chem 2007;103:734‑40.



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