Ameliorative effect of ethanolic Gymnema sylvestre extract on diabetic cardiomyopathy against streptozotocin‑induced diabetes in Wistar rats

Vinay Kumar, Uma Bhandari, Chakra Dhar Tripathi, Geetika Khanna

Abstract


Background: Diabetes leads to a cardiomyopathy characterized by myocyte loss. Streptozotocin (STZ)‑induced diabetic
cardiomyopathy is characterized by decreased left ventricular contractility and diminished ventricular compliance with marked
abnormal systolic and diastolic function. Aim: The ameliorative effect of ethanolic Gymnema sylvestre extract (GSE) was evaluated in diabetic cardiomyopathy against STZ‑induced diabetes. Materials and Methods: Diabetes was induced by a single intravenous injection of (STZ, 45 mg/kg) in male Wistar rats. Blood pressure, serum lactate dehydrogenase (LDH), glucose apolipoprotein B and lipids as well as heart weight, caspase‑3, sodium potassium adenosine triphosphatase Na+ K+ ATPase, and DNA laddering were determined. Results and Conclusions: Administration of GSE (120 mg/kg/p.o.) treatment significantly (P < 0.01) reduced myocyte loss by suppressing the levels of cardiac caspase‑3, DNA laddering; mean arterial blood pressure and heart rate as well as serum LDH, glucose, apolipoprotein B, and lipids levels. Further, it increased the heart weight and cardiac Na+ K+ ATPase activity in diabetic rats.
The cardiomyopathy suppression is accompanied by decrease in cardiac caspase‑3 levels, DNA laddering, blood pressures, serum
LDH, apolipoprotein‑B and glucose. Thus, this present study reports the anti‑apoptotic potential of GSE in STZ‑induced diabetic cardiomyopathy.
Key words: Apolipoprotein‑B, caspase‑3, diabetic cardiomyopathy, DNA fragmentation, Gymnema sylvestre

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References


King H, Aubert RE, Herman WH. Global burden of diabetes,

‑2025: Prevalence, numerical estimates, and projections.

Diabetes Care 1998;21:1414‑31.

Mahgoub MA, Abd‑Elfattah AS. Diabetes mellitus and cardiac

function. Mol Cell Biochem 1998;180:59‑64.

Wu TG, Li WH, Lin ZQ, Wang LX. Effects of folic acid on cardiac

myocyte apoptosis in rats with streptozotocin‑induced diabetes

mellitus. Cardiovasc Drugs Ther 2008;22:299‑304.

Dandona P, Thusu K, Cook S, Snyder B, Makowski J,

Armstrong D, et al. Oxidative damage to DNA in diabetes mellitus.

Lancet 1996;347:444‑5.

Li YH, Zhuo YH, Lü L, Chen LY, Huang XH, Zhang JL, et al.

Caspase‑dependent retinal ganglion cell apoptosis in the rat model

of acute diabetes. Chin Med J (Engl) 2008;121:2566‑71.

Mann CL, Bortner CD, Jewell CM, Cidlowski JA.

Glucocorticoid‑induced plasma membrane depolarization

during thymocyte apoptosis: Association with cell shrinkage

and degradation of the Na(+)/K(+)‑adenosine triphosphatase.

Endocrinology 2001;142:5059‑68.

Saini KS, Thompson C, Winterford CM, Walker NI, Cameron DP.

Streptozotocin at low doses induces apoptosis and at high doses

causes necrosis in a murine pancreatic beta cell line, INS‑1.

Biochem Mol Biol Int 1996;39:1229‑36.

D’Souza T, Mengi SA, Hassarajani S, Chattopadhayay S.

Efficacy study of the bioactive fraction (F‑3) of Acorus calamus in

hyperlipidemia. Indian J Pharmacol 2007;39:196‑200.

Moro CO, Basile G. Obesity and medicinal plants. Fitoterapia

;71:S73‑82.

Murray MT, Pizzorno Jr JE. Diabetes mellitus In: Pizzorno

Jr JE, Murray MT, editors. Text Book of Natural Medicine 2nd ed.

New York: Churchill Livingstone; 1999. p. 1193‑218.

Fushiki T, Kojima A, Imoto T, Inoue K, Sugimoto E. An extract

of Gymnema sylvestre leaves and purified gymnemic acid inhibits

glucose‑stimulated gastric inhibitory peptide secretion in rats.

J Nutr 1992;122:2367‑73.

Bishayee A, Chatterjee M. Hypolipidemic and antiatherosclerotic

effects of Gymnema sylvestre leaf extract in albino rats fed on highfat diet. Phytother Res 1994;8:118‑20.

Shigematsu N, Asano R, Shimosaka M, Okazaki M. Effect of

administration with the extract of Gymnema sylvestre R. Br leaves

on lipid metabolism in rats. Biol Pharm Bull 2001;24:713‑7.

Malik JK, Manvi FV, Alagawadi KR, Noolvi M. Evaluation of

anti‑inflammatory activity of Gymnema sylvestre leaves extract in

rats. Int J Green Pharmacy 2008;2:114‑5.

World health organization. Quality Control Methods for Medicinal

Plant Material. Geneva: WHO; 1998. p. 28.

Alam MM, Javed K, Jafri MA. Effect of Rheum emodi (Revand

Hindi) on renal functions in rats. J Ethnopharmacol 2005;96:121‑5.

Gurtu V, Kain SR, Zhang G. Fluorometric and colorimetric

detection of caspase activity associated with apoptosis. Anal

Biochem 1997;251:98‑102.

Bonting SL. Sodium potassium activated adenosine triphosphatase

and cation transport. In: Membrane and Ion Transport. Bittar EE,

editor. London: Interscience Wiley; 1970. p. 257‑63.

Ling H, Lou Y. Total flavones from Elsholtzia blanda reduce infarct

size during acute myocardial ischemia by inhibiting myocardial

apoptosis in rats. J Ethnopharmacol 2005;101:169‑75.

Marklund S, Marklund G. Involvement of the superoxide anion

radical in the autoxidation of pyrogallol and a convenient assay

for superoxide dismutase. Eur J Biochem 1974;47:469‑74.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal

tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351‑8.

Teuscher A, Egger M, Herman JB. Diabetes and hypertension.

Blood pressure in clinical diabetic patients and a control

population. Arch Intern Med 1989;149:1942‑5.

Crane RK. The gradient hypothesis and other models of

carrier‑mediated active transport. Rev Physiol Biochem Pharmacol

;78:99‑159.

Guignot L, Mithieux G. Mechanisms by which insulin, associated

or not with glucose, may inhibit hepatic glucose production in the

rat. Am J Physiol 1999;277:E984‑9.

Laybutt R, Hasenkamp W, Groff A, Grey S, Jonas JC, Kaneto H, et al.

beta‑cell adaptation to hyperglycemia. Diabetes 2001;50:S180‑1.

Aguilar‑Diosdado M, Parkinson D, Corbett JA, Kwon G,

Marshall CA, Gingerich RL, et al. Potential autoantigens in

IDDM. Expression of carboxypeptidase‑H and insulin but not

glutamate decarboxylase on the beta‑cell surface. Diabetes 1994;

:418‑25.

Morita SY, Deharu Y, Takata E, Nakano M, Handa T. Cytotoxicity

of lipid‑free apolipoprotein B. Biochim Biophys Acta

;1778:2594‑603.

Arechabala B, Coiffard C, Rivalland P, Coiffard LJ,

de Roeck‑Holtzhauer Y. Comparison of cytotoxicity of various

surfactants tested on normal human fibroblast cultures using

the neutral red test, MTT assay and LDH release. J Appl Toxicol

;19:163‑5.

Liu W, Staecker H, Stupak H, Malgrange B, Lefebvre P,

Van De Water TR. Caspase inhibitors prevent cisplatin‑induced

apoptosis of auditory sensory cells. Neuroreport 1998;9:

‑14.




DOI: http://dx.doi.org/10.22377/ijgp.v7i1.288

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