Comparative and combined studies on anti‑ulcer effect of two plant extracts in experimental models of gastric ulcer in SD rats

Gangireddy Ramana, Siva Reddy Challa, Ch. V. Rao

Abstract


Objective: The present study was designed to carry out comparative and combined effect anti-ulcer studies of Ficus racemosa Linn. (Family: Moraceae) (Gular) fruit extract and Aegle marmelos (Linn.) Corr. (family: Rutaceae) root extract in various experimental models of ulcer. Materials and Methods: Gastro-protective studies were carried out with individual treatments of Ficus racemosa fruit extract (FRFE) and Aegle marmelos root extract (AMRE) and combination of both in four standard experimental models of ulcer like pylorus ligation (PL)-induced ulcers, Aspirin (ASP)-induced ulcers, Cold-restraint stress (CRS)-induced ulcers, and Ethanol (EtOH)- induced ulcers in SD rats. Gastric secretion parameters, gastric mucosal parameters and anti-oxidant parameters were estimated.
Results: Both FRFE and AMRE have demonstrated the efficacy in different models of ulcer in the following order: PL-induced gastric ulcers > ASP-induced gastric ulcers > EtOH-induced gastric ulcers > CRS-induced gastric ulcers. Both have shown similar degree of efficacy in almost all the models. Results clearly implied that the three combinations A1-50 mg/kg (25 mg of FRFE + 25 mg of AMRE), A2-75 mg/kg (37.5 mg of FRFE + 37.5 mg of AMRE) and A3-100 mg/kg (50 mg of FRFE + 50 mg of AMRE) show significant anti-ulcer effect in CRS-induced ulcer model. Summary: Combination of both FRFE and AMRE have exhibited remarkable antiulcer effect at even lower doses of equal proportions (1:1) in comparison with individual treatment studies, which also have shown good efficacy but much higher doses are required. This study highlights the importance of combined herbal formulations that help
to facilitate the efficacy as well as safety of drugs.
Key words: Aegle marmelos, anti‑ulcer effect, Ficus racemosa, gastroprotection

Full Text:

PDF

References


Lam EK, Tai EK, Koo MW, Wong HP, Wu WK, Yu L, et al.

Enhancement of gastric mucosal integrity by Lactobacillus

rhamnosus GG. Life Sci 2007;80:2128‑36.

Moraes TM, Kushima H, Moleiro FC, Santos RC, Rocha LR,

Marques MO, et al. Effects of limonene and essential oil from Citrus

aurantium on gastric mucosa: Role of prostaglandins and gastric

mucus secretion. Chem Biol Interact 2009;180:499‑505.

Kirtikar KR, Basu BD. In: Blatter E, Caius JF, Mhaskar KS, editors.

Indian medicinal plants. Vol. 1 2nd ed. Allahabad, India: Lalit

Mohan Basu Publications; 1935. p. 499.

Jahan IA, Nahar N, Mosihuzzaman M, Rokeya B, Ali L, Azad

Khan AK, et al. Hypoglycaemic and antioxidant activities of Ficus

racemosa Linn. fruits. Nat Prod Res 2009;23:399‑408.

Rao RB, Anupama K, Swaroop KR, Murugesan T, Pal M,

Mandal SC. Evaluation of anti‑pyretic potential of Ficus racemosa

bark. Phytomedicine 2002;9:731‑33.

Rao RB, Murugesan T, Pal M, Saha BP, Mandal SC. Anti‑tussive

potential of methanolic extract of stem bark of Ficus racemosa Linn.

Phytother Res 2003;17:1117‑8.

Vijaya C, Ramanathan M, Suresh B. Lipid lowering activity

of ethanolic extract of leaves of Aegle marmelos (Linn.) in

hyperlipidemic models of wistar albino rats. Indian J Exp Biol

;47:182‑5.

Pratheepa V, Madasamy D, Sukumaran N. Immunomodulatory

activity of Aegle marmelos in freshwater fish (Catla catla) by

non‑specific protection. Pharm Biol 2010;49:73‑7.

Sanyal AK, Pandey BL, Goel RK. The effect of a traditional

preparation of copper, tamrabhasma, on experimental ulcer and

gastric secretion. J Ethnopharmacol 1982;5:79‑89.

Goel RK, Das DG, Sanyal AK. Effect of vegetable banana

powder on changes induced by ulcerogenic agents in dissolved

mucosubstances of gastric juice. Indian J Gastroenterol 1985;

:249‑51.

Amar A, Sanyal AK. Immobilization stress in rats: effects on

rectal temperature and possible role of brain monoaminase in

hypothermia. Psychopharmacology (Berl) 1981;73:157‑60.

Hollander D, Tarnawski A, Krause WJ, Gerely H. Protective effect

of sucralfate against alcohol‑induced gastric mucosal injury in the

rat. Macroscopic, histologic, ultrastructural, and functional time

sequence analysis. Gastroenterology 1985;88:366‑74.

Debnath PK, Gode KD, Das DG, Sanyal AK. Effect of propranolol

on gastric secretion in albino rats. Br J Pharmacol 1974;51:213‑6.

Winzler RJ. Determination of serum glycoproteins. Methods

Biochem Anal 1958;2:279‑311.

Dische Z, Borenfreund E. A spectroscopic method for the micro

determination of hexosamine. J Biol Chem 1950;184:517‑22.

Dische Z, Shettles LB. A specific color reaction for methylpentoses

and a spectrophotometric micromethod for their determination.

J Biol Chem 1948;175:595‑603.

Warren L. The thiobarbituric acid assay of sialic acids. J Biol Chem

;234:1971‑5.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement

with the Folin phenol reagent. J Biol Chem 1951;193:265‑75.

Das D, Banerjee RF. Effect of stress on the antioxidant enzymes

and gastric ulceration. Mol Cell Biochem 1993;125:115‑25.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxidase in animal

tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351‑8.

Aebi H. Catalase. In: Bergmeyer HU, editor. Methods in enzymatic

analysis. Vol. 3. New York: Academic Press; 1983. p. 276.

Organization for Economic Cooperation and Development

(OECD). OECD Guidelines for Testing of Chemicals. Acute Oral

Toxicity. OECD, No. 401. Paris France: OECD; 1993.

Miller TA, Henagan JM. Indomethacin decreases resistance of

gastric barrier to disruption by alcohol. Dig Dis Sci 1984;29:141‑9.

Sairam K, Rao ChV, Babu MD, Kumar KV, Agarawal VK, Goel RK.

Antiulcerogenic activity of methanolic extract of Emblica officinalis:

An experimental study. J Ethnopharmacol 2002;82:1‑9.

Cho CH, Ogle CW. Cholinergenic‑ mediated gastric mast cell

degranulation with subsequent histamine H1 and H2 ‑ receptor

activation in stress ulceration in rats. Eur J Pharmacol 1979;55:23‑33.

Hase T, Moss BJ. Microvascular changes of gastric mucosa in the

development of stress ulcer in rats. Gastroenterology 1973;65:

‑34.

Aguwa CN, Nwako SO. Preliminary studies of the root extracts

of Nauclea latifolia Smith, for anti‑ulcer properties. Niger J Pharm

Sci 1988;48:16‑23.

Suarez J, Herrera MD, Marhuenda E. Hesperidin and neohesperidin

dihydrochalcone on different experimental models of induced

gastric ulcer. Phytother Res 1998;50:616‑8.

Mizui T, Sato H, Hirose F, Doteuchi M. Effect of antiperoxidative

drugs on gastric damage induced by ethanol in rats. Life Sci

;41:755‑63.

Bagchi M, Milnes M, Williams C, Balmoori J, Ye X, Stohs S, et al.

Acute and chronic stress‑induced oxidative gastrointestinal

injury in rats and the protective ability of a novel grape seed

proanthocyanidin extract. Nutr Res 1999;19:1189‑99.

Bagchi D, Carryl OR, Tran MX, Krohn RL, Bagchi DJ, Garg A,

et al. Stress, diet and alcohol‑induced oxidative gastrointestinal

mucosal injury in rats and protection by bismuth subsalicylate.

J Appl Toxicol 1998;18:3‑13.




DOI: http://dx.doi.org/10.22377/ijgp.v6i2.251

Refbacks

  • There are currently no refbacks.