Determination of active substances of the “Propolis-Derma” pharmaceutical compositions by reverse-phase high-performance liquid chromatographic method

O. S. Shpychak


Aim: This study presents the material devoted to working out the methods of identification and quantitative determination of active substances of medicated products “Propolis-Derma” (“Propolis-PNH,” “Propolis-PSC,” and “Propolis-PCD”) with antifungal, antimicrobial, and keratolytic activity, created for the treatment of dermatomycoses, pityriasis versicolor, as well as diseases caused by yeast-like fungi of Candida albicans. Materials and Methods: As a result, the conditions, methods, and opportunity have presented for the quantitative determination of the content of active pharmaceutical ingredients of “Propolis-Derma” pharmaceutical compositions by the method of the reverse-phase high-performance liquid chromatography on the basis of milichrom A-02 chromatograph. Results and Discussion: By comparing the retention times and the values of spectral ratios of the peaks obtained with such data in the database of chromatograph, the presence of naftifine hydrochloride has been confirmed in the composition of the medicated product “Propolis-PNH,” chlorquinaldol and salicylic acid – in the composition of “Propolis-PSC,” and chlorhexidine digluconate – in “Propolis-PCD” composition. In addition, in each of five series of experimental samples, the presence of phenolic propolis compounds which are part of the propolis tincture has been established as one of the main active components of the pharmaceutical compositions offered. Conclusions: It has been defined that experimental data on the content of biologically active substances of pharmaceutical compositions “Propolis-PNH,” “Propolis-PSC,” and “Propolis-PCD” are well correlated with the composition of the medicated preparations developed and the literary data on the chemical structure and composition of the basic substances, and the method offered gives the possibility to carry out standardization of the composition of the medicine developed, namely, “Propolis-Derma.”

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