Fate of biomolecules during carbon tetrachloride induced oxidative stress and protective nature of Ammoniac baccifera Linn.: A natural antioxidant

G. Lavanya, R. Sivajyothi, M. Manjunath, P. R. Parthasarathy

Abstract


Ethanol extract of Ammannia baccifera was studied for its protective nature against the oxidative damage of lipids, proteins and DNA in carbon tetrachloride (CCl4)-induced toxicity in rats. CCl4 administration to albino Wistar rats increased the levels of lipid peroxidation, protein carbonyls and decreased the levels of total sulfhydryls. CCl4 also induced the elevation of DNA damage measured by the comet assay. The study revealed that the administration of the ethanol extract of A. baccifera to CCl4 intoxicated rats could significantly (P , 0.01) decrease the levels of lipid peroxidation, protein carbonyls and increased the levels of total sulfhydryls in a dose-dependent manner. It was also found that the ethanol extract of A. baccifera prevent the CCl4-induced elevation of DNA damage in hepatocytes. These results suggest that treatment with the ethanol extract of A. baccifera can minimize the deleterious effects caused by CCl4 through its strong antioxidative and free radical scavenging properties.
Key words: Antioxidants, comet assay, DNA, protein carbonyls, total sulfhydryls

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DOI: http://dx.doi.org/10.22377/ijgp.v3i4.105

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