Evaluation of the immunomodulatory activity of ethanolic extract of the stem bark of Bauhinia variegata Linn

M. M. Ghaisas, S. A. Shaikh, A. D. Deshpande


To evaluate the immunomodulatory activity of the ethanolic extract of the stem bark of Bauhinia variegata Linn, to justify the traditional claim endowed upon this herbal drug as a rasayana in Ayurveda. The effect of the ethanolic extract of the stem bark of Bauhinia variegata (EBV) on the primary and secondary antibody responses was evaluated by the humoral antibody response for a specific immune response. The effect of EBV on the phagocytic activity was evaluated by the carbon clearance test and neutrophil activation was evaluated by the neutrophil adhesion test for a nonspecific immune response. The data was analysed by one-way ANOVA followed by Tukey-Kramar multiple comparison tests. On oral administration, EBV showed a significant increase in the primary and
secondary humoral antibody responses, by increasing the hemagglutinating antibody titre at doses of 250 and 500 mg/kg/p.o. There was a significant increase in the phagocytic index and percentage neutrophil adhesion at doses of 250 and 500 mg/kg/p.o. The present study reveals that the ethanolic extract of the stem bark of Bauhinia variegata Linn holds a promise as an immunomodulatory agent, which acts probably by stimulating both the specific and nonspecific arms of immunity.
Key words: Antibody titre, Bauhinia variegata, immunomodulator, neutrophil adhesion, phagocytic activity, rasayana

Full Text:



Wagner H, Proksh A. Immunostimulatory drugs of fungi and higher

plants. In: Wagner H, editor. Economic and medicinal plant research.

Vol. 1, London, New York: Academic Press; 1985. p. 113.

Atal CK, Sharma ML, Kaul A, Khajuria A. Immunomodulating

agents of plant Origin, I: Preliminary screening. J Ethnopharmacol


Agarwal SS, Singh VK. Immunomdulators: A review of studies on

Indian medicinal plants and synthetic peptides, Part-I: medicinal

plants. Proc Indian Natl Sci Acad 1999;65:179-204.

Thatte UM, Dahanurkar SA. Rasayana concept: Clues from

immunomodulatory therapy. In: Upadhyay SN, editor.

Immunomodulation. New Delhi: Narosa Publishing House;

p. 141.

Kirtikar KR, Basu B. Indian Medicinal Plants. Vol. 2, Dehradun:

International Book Publisher; 1993. p. 898-900.

Nadkarni AK. Indian Materia Medica. Vol. 1, New Delhi: Popular

Directorate, CSIR; 2001. p. 56.

Parekh J, Karathia N, Chandra S. Evaluation of antibacterial

activity and phytochemical analysis of Bauhinia variegata L bark.

Afr J Biomed Res 2006;9:53-6.

Rajkapoor B, Jayakar B, Murugesh N, Sakthisekaran D.

Chemoprevention and cytotoxic effect of Bauhinia variegata against

N-nitrosodiethylamine induced liver tumors and human cancer

cell lines. J Ethnopharmacol 2006;104:407-9.

Rajkapoor B, Jaykar B, Murugesh N. Antitumour activity of

Bauhinia variegata on daltons ascitic lymphoma. J Ethnopharmacol


Rajkapoor B, Jayakar B, Murugesh, Anandan R. Anti-ulcer effect of

Bauhinia variegata Linn. In rats. J Nat Remedies 2003;3:215-7.

Ali MS, Azhar I, Amtul Z, Ahmad VU, Usmanghani K.

Antimicrobial screening of Some Caesalpiniaceae. Fitoterpia


Joharapurkar AA, Deode NM, Zambad SP, Umathe SN.

Immunomodulatory activity of alcoholic extract of Rubia cordifolia

LINN. Indian Drugs 2003;40:179-81.

Bafna MR, Mishra SH. Immunomodulatory activity of methanol

extract of flower-heads of Sphaeranthus indicusLinn. J Herb

Pharmacother 2007;7:25-37.

Mallurwar VR, Johrapurkar AJ, Durgakar NJ. Studies on

immunomodulatory activity of Mucuna pruriens. Indian J Pharma

Educ Res 2006;40:205-7.

Fulzele SV, Bhurchandi PM, Kanoje VM, Joshi SB, Dorle AK.

Immunostimulant activity of Asthmangal ghrita in rats. Indian J

Pharmacol 2002;34:194-7.

Goldsby RA, Kindt TJ, Osborne BA, Kuby J. Immunology. 5th ed.

New York: W. H. Freeman and Co; 2003. p. 1-25.

Dale MM, Formanj C. Text-book of Immunopharmacology. 2nd ed.

Oxford: Blackwell Scientific Publication; 1989. p. 14.

Hepner GH, Calabresi P. Selective suppression of humoral

immunity by antineoplastic drugs. Ann Rev Pharmacol Toxicol


Lagrange PH, Mackaness GB, Miller TE. Potentiation of

T-cell-mediated immunity by selective suppression of antibody

formation with cyclophosphamine. J Exp Med 1974;139:1529-39.

De P, Dasgupta SC, Gomes A. Immunopotentiating and

immunoprophylactic activities of immune 21: A polyherbal

product. Indian J Pharmacol 1998;30:163-8.

DOI: https://doi.org/10.22377/ijgp.v3i1.60


  • There are currently no refbacks.