Evaluation of antioxidant activity of essential oil from Ajwain (Trachyspermum ammi) seeds

Sreemoyee Chatterjee, Nandini Goswami, Neha Kothari

Abstract


Background: Free radicals provoke chain reactions within the cell and cause damage to the cell. Oxidation of bio molecules such as carbohydrate, protein, lipid and nucleic acid leads to the production of free radicals which induce the onset of disease. Antioxidants scavenge these free radicals, thereby protecting the cell from damage. Phenolic compounds found in varieties of seeds and fruits are thought to have antioxidant activities. Aim: The present study was designed to examine the in vitro antioxidant activity of Trachyspermum ammi (Ajwain) essential oil. Materials and Methods: The antioxidant activity of the oil was assessed by using 2, 2‑diphenyl‑1‑picryl‑hydrazyl (DPPH), H2O2 radical scavenging activity and ferric reducing antioxidant power (FRAP). The values were compared with those obtained with standard antioxidant, ascorbic acid. Results: It was determined that the essential oil possessed a high degree of FRAP followed by a good DPPH radical scavenging activity and a moderate H2O2 radical scavenging activity. Conclusion: This study concludes that the essential oil of Ajwain could contribute as a highly significant bio resource of antioxidants to be used
in our day‑to‑day life in food and pharmaceutical industry.
Key words: 2,2‑diphenyl‑1‑picryl‑hydrazyl, antioxidant activity, essential oil, ferric reducing antioxidant power, H2O2, thymol,
Trachyspermum ammi

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References


Halliwell B. The antioxidant paradox. Lancet 2000;355:1179‑83.

Young IS, Woodside JV. Antioxidants in health and disease. J Clin

Pathol 2001;54:176‑86.

Sharma NK, Sangh P, Priyanka, Jha KK, Singh HK, Shrivastav AK.

Free radical scavenging activity of methanolic extract of Luffa

cylindrica leaves. Int J Green Pharm 2012;6:231‑6.

Maxwell SR. Prospects for the use of antioxidant therapies. Drugs

;49:345‑61.

Abirami P, Gomathinayagam M, Panneerselvam R. Antioxidant

activity of the medicinal plant Enicostemma littorale Blume. Int J

Green Pharm 2011;5:342‑5.

Dapkevicius A, Venskutonis R, VanBeek TA, Linssen J. Antioxidant

activity of extracts obtained by different isolation procedures

from some aromatic herbs grown in Lithuania. J Sci Food Agr

;77:140‑6.

Azuma Y, Nakawo KM, Jiankan H, Watanabe O. Metting induced

relocation of ions in glacier and in a seasonal snow‑pack. IAHS

Pub 1995;223:287.

Velioglu Y, Mazza G, Gao L, Oomah BD. Antioxidant activity and

total phenolics in selected fruits, vegetables and grain products.

J Agric Food Chem 1998;46:4113‑7.

Sasaki YF, Kawaguchi S, Kamaya A, Ohshita M, Kabasawa K,

Iwama K, et al. The comet assay with 8 mouse organs: Results with

currently used food additives. Mutat Res 2002;519:103‑19.

Ormancey X, Sisalli S, Coutiere P. Formulation of essential

oil in functional perfumery. Perfums Cosmetiques Actualites

;157:30‑40.

Cieslik E, Greda A, Adamus W. Contents of polyphenols in fruit

and vegetables. Food Chem 2006;94:135‑42.

Kaur C, Kapoor HC. Antioxidant activity and total phenolic content

of some Asian vegetables. Int J Food Sci Tech 2002;37:153‑62.

Davidson A, Jaine T. The Oxford Companion to Food. USA: Oxford

University Press; 2006.

Khanuja SP. Formulation comprising thymol useful in the

treatment of drug resistance bacterial infection. New Delhi: United

State Patent No US 6,824,795 b2, CCIR; 2004.

Javed IM, Akhtar T, Khaliq MZ, Khan G, Muhammad M.

Antihyperlipidaemic effect of Trachyspermum ammi (Ajwain) in

rabbits. In: Faisalabad: Proc 33rd All Pakistan Science Conference

University of Agriculture 2002. p. 80‑1.

Gilani AH, Jabeen Q, Ghayur MN, Janbaz KH, Akhtar MS. Studies

on the antihypertensive, antispasmodic, bronchodilator and

hepatoprotective activities of the Carum copticum seed extract.

J Ethnopharmacol 2005;98:127‑35.

Ahsan SK, Shah AH, Tanira MO, Ahmad MS, Tariq M, Ageel AM.

Studies on some herbal drugs used against kidney stones in Saudi

folk medicine. Fitoterapia 1990;61:435‑8.

Boskabady MH, Jandaghi P, Kiani S, Hasanzadeh L. Antitussive

effect of Carum copticum in guinea pigs. J Ethnopharmacol

;97:79‑82.

Murthy PS, Borse BB, Khanum H, Srinivas P. Inhibitory effects

of Ajwain (Trachyspermum ammi) ethanolic extract on A. orchaceus

growth and ochratoxin production. Turk J Biol 2009;33:211‑7.

Park IK, Kim J, Lee SG, Shin SC. Nematicidal Activity of Plant

Essential Oils and Components From Ajowan (Trachyspermum

ammi), Allspice (Pimenta dioica) and Litsea (Litsea cubeba) Essential

Oils Against Pine Wood Nematode (Bursaphelenchus Xylophilus).

J Nematol 2007;39:275‑9.

Mathew N, Misra‑Bhattacharya S, Perumal V, Muthuswamy K.

Antifilarial lead molecules isolated from Trachyspermum ammi.

Molecules 2008;13:2156‑68.

Tsimidou M, Boskou D. Antioxidant activity of essential oils

from the plants of the Lamiaceae family. Charalambous G, editor.

Species, Herbs and Edible Fungi. Amsterdam: Elsevier; 1994.

p. 273‑8.

Awadhesh K, Mishra RK, Srivastava S, Tiwari AK, Pandey A,

Shukla AC. Role of phylogenetic analysis for anti‑bacterial activity

of essential oil of Trachyspermum ammi L. against water borne

pathogens. Adv Environ Res 2011;5:1271‑8.

Ashnagar A, Naseri G, Ramazani M. Characterization of the major

chemical compounds foung in Thymus vulgaris plant grown wildly

in Chahar Mahal and Bakhtiari province of Iran. Int J PharmTech

Res 2011;3:1‑4.

Vilasrao JK, Joshi YM, Sawant HP, Jadhav TA. Free radical

scavenging activity of aqueous solution of black salt. Int J Pharm

Pharm Sci 2010;2:95‑6.

Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and

inhibition of intercellular communication by antioxidant catechins

isolated from Chinese green tea. Carcinogenesis 1989;10:1003‑8.

Benzie IF, Strain JJ. Ferric reducing/antioxidant power assay:

Direct measure of total antioxidant activity of biological fluids

and modified version for simultaneous measurement of total

antioxidant power and ascorbic acid concentration. Methods

Enzymol 1999;299:15‑27.

Cao G, Alessio HM, Cutler RG. Oxygen‑radical absorbance capacity

assay for antioxidants. Free Radic Biol Med 1993;14:303‑11.

Yang J, Guo J, Yuan J. In vitro antioxidant properties of rutin.

Lebensm Wiss Technol 2008;41:1060‐6.

Nabavi SM, Ebrahimzadeh MA, Nabavi SF, Jafari M. Free

radical scavenging activity and antioxidant capacity of Eryngium

caucasicum Trautv and Froripia subpinnata. Pharmacol online

(b);3:19‑25.

Ebrahimzadeh MA, Nabavi SF, Nabavi SM. Antioxidant activities

of methanol extract of Sambucus ebulus L. flower. Pak J Biol Sci

;12:447‑50.

Rice‑Evans CA, Miller NT, Paganga G. Antioxidant properties of

selected fruit seeds. Food Chem 1997;88:411‑7.

Oktay M, Culcin I, Kufrevioglu OI. Determination of in vitro

antioxidant activity of fennel (Foeniculum vulgare) seed extracts.

Lebensm Wiss Technol 2003;36:263‑71.




DOI: http://dx.doi.org/10.22377/ijgp.v7i2.312

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