A study to evaluate bacoside A in Brahmi Ghrita by HPTLC method

Jyoti S. Gubbannavar, H. M. Chandola, Renuka Kalyani, V. J. Shukla

Abstract


Context: Brahmi (Bacopa monnieri Linn. Pennel), the main ingredient of Brahmi Ghrita is a popular Medhya drug referred in Ayurvedic classics. Recent advances have established the action of bacoside, one of the constituents of Brahmi in various psycho‑neurological disorders. Aim: Present study was planned to detect bacoside A and to do quantitative and qualitative analysis of bacoside A in Brahmi Ghrita using standard bacoside A sample through HPTLC method. Materials and Methods: Stationary phase was precoatedsilica gel GF 254 and mobile phase was dichloromethane: methanol: water (4.5:1.0:0.1 v/v/v). The plate was scanned and quantified
at 254 nm for bacoside A. Result: The correlation co‑efficient of bacoside A was found to be 0.9989 or 20% in 5 μl Brahmi Ghrita
sample. Conclusion: The study confirms the presence of bacoside A in Brahmi Ghrita. The correlation coefficient of bacoside A was found to be 0.9989 or 20% in 5 μl Brahmi Ghrita sample. The simple, accurate and cost‑effective HPTLC method can be utilized for the routine analysis of quantitative determination of bacoside A in Brahmi Ghrita.
Key words: Bacoside A, Brahmi Ghrita, HPTLC

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DOI: http://dx.doi.org/10.22377/ijgp.v6i3.258

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