Molecular detection and sequencing of SHV gene encoding for extended-spectrum β-lactamases produced by multidrug resistance some of the Gram-negative bacteria

Abstract

Background and Objective: The increase in extended-spectrum β-lactamases (ESBLs) producing microbes in recent years has led to the great challenge for the clinician in the treatment. The study aims to investigate the molecular basis of ESBL encoding-resistant gene, SHV to modern β-lactams. Also, to detect gene sequencing and to compare their genetic relatedness utilizing phylogenetic analysis. Patients and Methods: A total of 100 clinical isolates are submitting after identification to phenotypic confirmatory double disk synergy technique. Polymerase chain reaction was performed for the determination of SHV gene, and sequencing analysis for the amplified gene is also achieved. Results: A total of 27 isolates, 3 (11.1%), 15 (55.55%), and 9 (33.0%) of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa were positive for a phenotypic and confirmatory test for ESBLs, respectively. SHV gene was detected only in 5 (31.25%) isolates of P. aeruginosa, 2 (12.5 %) E. coli, and 9 (56.25%) in K. pneumoniae. Conclusions: SHV gene plays an essential role in the resistance of ESBL producer isolates to new β-lactams. The sequencing of this gene revealed 98–99% compatibility range with the global standard gene in National Center of Biotechnology Information.