Molecular genetic tagging of wheat varieties genes resistant to Septoria tritici in northern Kazakhstan

Sandukash A. Babkenova

Abstract


Aim: The aim of the research was to isolate sources of wheat resistance to Septoria tritici and identify genes that provide resistance to this disease based on the methodology common for phytopathological and molecular genetic studies. Materials and Methods: During the study, 303 spring wheat varietiesof different ecological and geographical origin were screened. At this, 36 samples resistant to S. tritici were isolated at the artificial infectious background. Results: In consequence of molecular genetic tagging of resistance genes in 36 varieties of wheat, it was determined that the majority of the analyzed samples carried in their genotype resistance genes ineffective to S. tritici. Just resistance gene Stb2 was characterized by the moderate efficiency. Among the studied varieties with this gene, eight samples were identified: Strain 36/12-1, Laban, GN06600, MN 94382, SD 80-89, Roblin, Yesaul, and Nota. Field resistance against the disease can be provided by varieties with a large number of resistance genes, such as Laban and Krabat. Varieties of gene Stb8 with efficient resistance to the S. tritici pathogen are of the greatest interest for selection for immunity to S. tritici. Conclusion: In consequence of the conducted research, two varieties of Laban and A.C. crystal with an effective resistance gene Stb8 were identified. These varieties should be used in breeding process as resistance donors to S. tritici.

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DOI: http://dx.doi.org/10.22377/ijgp.v11i03.1153

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