Immunostimulatory effect of standardised alcoholic extract of green tea (Camellia sinensis L.) against cyclophosphamide‑induced immunosuppression in murine model

Mohammad Rafiul Haque, Shahid Husain Ansari

Abstract


Backgrounds: Tea, Camellia sinensis (L.) O. Kuntze (Theaceae) is one of the most widely consumed beverages in the world. It can be classified into three major types, depending on the level of fermentation, that is, green and white (unfermented), oolong (partially fermented) and black (fermented) tea. Each type of tea has a distinct composition, dependent on how the leaves are processed, as well as maturation, geographical location and agricultural practices. Green tea (GT), the least processed tea, is one of the highest studied and is ascribed to have the highest content of phenolic compounds. Aim: The current study was intended to evaluate the immunostimulatory potential of alcoholic extract of green tea (GTEA or GTAE) in cyclophosphamide (CP)‑induced immunosuppression in murine model. Materials and Methods: GTEA was standardised by high pressure thin liquid chromatography (HPTLC). CP (50 mg/kg, i.p.) was used to induce immunosuppression in mice. GTAE (50, 150 and 250 mg/kg) was administered orally for 14 day. Results: GTEA (150 and 250 mg/kg) treatment significantly (P < 0.001) elevated the thymus and spleen weight, haemagglutination titre and total leucocyte counts level and lowered the delayed type hypersensitivity response as compared with CP‑induced immunosupressed mice.
Conclusions: These observations suggest immunostimulatory effects of GTAE against CP‑induced immunosuppression in murine model.
Key words: Cyclophosphamide, delayed type hypersensitivity, green tea, Haemagglutination titre

Full Text:

PDF

References


Ziauddin M, Phansalkar N, Patki P, Diwanay S, Patwardhan B.

Studies on the immunomodulatory effect of Ashwagandha.

J Ethnopaharmacol 1996;50:69‑76.

Nishiguri C, Yorosh DB, Donawho C, Kripke ML. The immune

system in ultraviolet carcinogenesis. J Invest Dermatol Symp Proc

;1:143‑6.

Lakhashe S, Thakar M, Godbole S, Tripathy S, Paranjape R. HIV

infection in India: Epidemiology, molecular epidemiology and

pathogenesis. J Biosci 2008;33:515‑25.

Ramnath V, Girija K, Kuttan R. Immunomodulatory effect of Abrin

in normal mice. Amala Cancer Bul 2001;21:76‑81.

Patwardhan B, Kalbag D, Patki PS, Nagsampagi BA. Search

of immunomodulatory agents: A review. Indian Drugs

;28:348‑58.

Premanathan M, Rajendran S, Ramanathan T, Kathiresan K,

Nakashima H, Yamamoto N. A survey of some Indian

medicinal plants for anti‑human immunodeficiency virus (HIV)

activity. Indian J Med Res 2000;112:73‑7.

Mullurwar VR, Pathak, AK. Studies on immunomodulatory

activity of Ficus reigiosa. Indian J pharm Edu Res 2008;42:341‑3.

López V, Calvo MI. White tea (Camellia sinensis Kuntze) exerts

neuroprotection against hydrogen peroxide‑ induced toxicity in

PC12 cells. Plant Foods Hum Nutr 2011;66:22‑6.

De Mejia EG, Ramirez‑Mares MV, Puangpraphant S. Bioactive

components of tea: Cancer, inflammation and behavior. Brain

behav Immun 2009;23:721‑31.

Graham HN. Green tea composition, consumption, and

polyphenol chemistry. Prev Med 1992;21:334‑50.

Leung LK, Su Y, Chen R, Zang Z, Huang Y, Chen ZY. Theaflavins in

black and catechins in green tea are equally effective antioxidants.

J Nutr 2001;131:2248‑51.

Kim HJ, Jeon SM, Lee MK, Jung UJ, Shin SK, Choi MS. Antilipogenic

effect of green tea extract in C57BL/6J‑Lep ob/ob mice. Phytother

Res 2009;23:467‑71.

Salminen WF, Yang X, Shi Q, Greenhaw J, Davis K, Ali AA.

Green tea extract can potentiate acetaminophen‑induced

hepatotoxicity in mice. Food Chem Toxicol 2012;50:1439‑46.

International Conference on Harmonization (ICH) of Technical

Requirements for the Registration of Pharmaceuticals

for Human Use, Validation of analytical procedures, ICH‑Q2A,

Geneva; 1995.

McDonald S, Prenzler PD, Autolovich M, Robards K. Phenolic

content and antioxidant activity of olive extracts. Food Chem

;73:73‑84.

Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid

content in propolis by two complementary colorimetric methods.

J Food drug analysis 2002;10:178‑82.

Puri A, Saxena RP, Saxena KC, Srivastava V, Tandon JS.

Immunostimulant activity of Nyctanthes arbour‑tristis L.

J Ethnopharmacol 1994;42:31‑7.

Manjarekar PN, Jolly CI, Narayanan S. Comparative studies of the

immunomodulatory activity of Tinospora cordifolia and Tinospora

sinensis. Fitoterapia 2000;71:254‑7.

Vigila AG, Baskaran X. Immunomodulatory effect of coconut

protein on cyclophosphamide induced immune suppressed Swiss

albino mice. Ethnobot Leaflets 2008;12:1206‑12.

Agarwal R, Diwanay S, Patki P, Patwardhan B. Studies on

immunomodulatory activity of Withania somnifera (Ashwagandha)

extracts in experimental immune inflammation. J Ethnopharmacol

;67:27‑35.

Mitsuoka A, Mitsuo B, Morikawa S. Enhancement of DTH by

depletion of suppressor T‑cells with cyclophosphamide in mice.

Nature 1976;262:77‑9.

Mitra SK, Gupta M, Sarma, DN. Immunomodulatory effect of

VIM‑133. Phytother Res 1999;13:341‑3.

Benacerraf B. A hypothesis to relate the specificity of T lymphocytes

and the activity of I region specific Ir genes in macrophages and

borrower lymphocytes. J Immunol 1978;120:1809‑32.

Makare N, Bodhankar S, Rangari V. Immunomodulatory activity of

alcoholic extract of Magnifera indica L.in mice. J Ethanopharmacol

;78:133‑49.

De La Fuente M, Victor VM. Anti‑oxidants as modulators of

immune function. Immunol Cell Biol 2000;78:49‑54.

Horrigan LA, Kelly JP, Connor TJ. Immunomodulatory effects of

caffeine: Friend or foe. Pharmacol Ther 2006;111:877‑92.

Matsunaga K, Friedman H, Yamamoto Y. Epigallocatechin

Gallate, a Potential Immunomodulatory Agent of Tea

Components, Diminishes Cigarette Smoke Condensate‑Induced

Suppression of Anti‑Legionella pneumophila Activity and

Cytokine Responses of Alveolar Macrophages. Clin Diagn Lab

Immunol 2002;9:864‑71.




DOI: http://dx.doi.org/10.22377/ijgp.v8i1.354

Refbacks

  • There are currently no refbacks.