Influence of altitudinal variation on the anti‑oxidant capacity of essential oil of Syzygium densiflorum from Southern Western Ghats, India

Saranya Jothiramshekar, Eganathan Palanisami, Sujanapal Puthiyapurayil


Background: Syzygium densiflorum is a vulnerable tree species belonging to the Myrtaceae family. Objective: To investigate the influence of altitudinal variation on the anti‑oxidant potential of leaf essential oil of Syzygium densiflorum. Materials and Methods: The leaf essential oil has been isolated using hydrodistillation process and their scavenging ability was determined using five in vitro assays such as
2, 2‑diphenyl‑1‑picrylhydrazyl (DPPH), ferric reducing anti‑oxidant power (FRAP), 2, 2'‑azino‑bis (3‑ethylbenzothiazoline‑6‑sulphonic
acid) (ABTS), hydrogen peroxide (H2O2) and hydroxyl radical scavenging assays. Statistical analysis: The results were analysed statistically using one‑way analysis of variance (ANOVA) following Duncan’s multiple range test. Results: Leaves from lower altitude showed higher activity against hydrogen peroxide, hydroxyl radical and ferric ion and moderate activity against DPPH and ABTS free radicals. Leaves from higher altitude essential oil exhibits potent activity against hydrogen peroxide and hydroxyl radical compared with the standard. Conclusions: Comparatively lower altitude leaf essential oil showed potent anti‑oxidant activity confirming the fact that altitudinal variations have profound effect on the anti‑oxidant potential of Syzygium densiflorum.
Key words: Altitudinal variations, anti‑oxidant capacity, essential oil, hydrodistillation, myrtaceae, Syzygium densiflorum,
vulnerable tree

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