Physico‑chemical standardization of Habbe Shifa: A polyherbal Unani formulation with modern techniques

Asira Tarannum, Shariq Shamsi, Roohi Zaman


Background: Habbe Shifa (HS) is an important pharmacopoeial Unani formulation, which is used in the treatment of Humma (fever), Iya (fatigue), Tashannuje rewi (pulmonary spasm) and Zeequn Nafas (asthma) and opium deaddiction. Aim: The physico‑chemical standards of HS were established in the present study. Materials and Methods: HS was prepared with ingredients of particle size 150 µm (100 mesh sieve), 5% w/w Gum Acacia mucilage was used as binder, dried at a temperature 90°C for 120 min and finally evaluated for different physico‑chemical parameters to develop standards for HS. Results and Conclusion: Physico‑chemical standards of HS were observed as characteristic brown colour, spherical shape, hard in texture, odourless and bitter in taste; average weight 242.95 ± 1.53 mg; diameter 7.33 ± 0.16 mm; hardness 3.5 ± 0.00 kg/cm; friability 0.02 ± 0.003%; pH value in 1% and 10% aqueous
solution 6.22 ± 0.06 and 5.39 ± 0.008 respectively; percentage loss of weight on drying at 105°C 6.63 ± 0.12%; total ash, acid insoluble ash and water soluble ash 5.33 ± 0.16, 0.95 ± 0.05 and 1 ± 0.00% respectively and total alkaloid 0.65 ± 0.01% and Rf values in the thin layer chromatography in ethanolic extract in hexane: Acetone (7.6:2.4) solvent system were 0.25, 0.78 and in hexane: Diethyl ether (4:6) solvent system were 0.58, 0.89. The results obtained for the various physico‑chemical tests of lab sample of HS may be taken as standard parameter for future reference and help in setting up regulatory limit to assure the quality of Unani medicine.
Key words: Habbe Shifa, physico‑chemical, standardization

Full Text:



Sharma A, Shanker C, Tyagi LK, Singh M, Rao CV. Herbal medicine

for market potential in india: An overview. Acad J Plant Sci


Yadav NP, Dixit VK. Recent approaches in herbal drug

standardization. Int J Integr Biol 2008;2:195‑202.

Calixto JB. Efficacy, safety, quality control, marketing and

regulatory guidelines for herbal medicines (phytotherapeutic

agents). Braz J Med Biol Res 2000;33:179‑89.

Arzani HM. Qarabadeene Quadri. New Delhi: Ejaz Publishing

House; 1998. p. 26.

Anonymous. National Formulary of Unani Medicine. (English

Edition). Part I. New Delhi: Ministry of Health and Family Welfare;

p. 31.

Said HM. Hamdard Pharmacopeia of Eastern Medicine. Delhi: Sri

Satguru Publications; 1997. p. 106.

Tajuddin M, Khan NA, Tajuddin, Afzal M. Analgesic,

anti‑convulsant and anti‑pyretic effects of a Unani compound

formulation, Habbe Shifa. Indian J Tradit Knowl 2007;6:315‑8.

Anonymous. Remington: The Science and Practice of Pharmacy,

Vol. 1. 21st ed. Noida (Indian Edition): Distributed by B.I

Publications Pvt. Ltd.; 2006. p. 916‑7.

Anonymous. Indian Pharmacopoeia. Vol. 1. Ghaziabad: Indian

Pharmacopoeia Commision; 2007. p. 20.

Waring EJ. Pharmacopoeia of India. Delhi: Asiatic Publishing

House; 2010. p. 62.

Lachman L, Lieberman HA, Kanig JL. The Theory and Practice of

Industrial Pharmacy. 3rd ed. Mumbai: Varghese Publishing House;

p. 297‑300.

Dandagi PM, Halakatti PK, Mastiholimath VM, Patil MB,

Manvi FV. Rapidly disintegrating Domperidone tablets. Indian

Drugs 2006;43:594‑7.

Vijaya KS, Mishra DN. Rapidly disintegration oral tablets of

Meloxicam. Indian Drugs 2006;43:117‑21.

Anonymous. Physico‑chemical standards of Unani formulations.

Part 4. New Delhi: Central Council for Research in Unani Medicine;

p. 142‑4.

Afaq SH, Tajuddin, Siddiqui MM. Standardization of Herbal

Drugs. Aligarh: A.M.U. Press; 1994. p. 33‑4, 41‑2.

Anonymous. Quality Control Methods for Medicinal Plant

Materials. Geneva: World Health Organization; 1998.

Anonymous. British Pharmacopoeia. Blumsberg Square, London:

General Medical Council. Pharmaceutical Press; 1968. p. 1276‑7,


Anonymous. Standardization of single Drugs of Unani Medicine.

Part III. New Delhi: Central Council for Research in Unani

Medicine; 1987. p. 261‑4.

Sutharsingh R, Kavimani S, Jayakar B, Uvarani M,

Thangathirupathi A. Quantitave phytochemical estimation and

antioxidant studies on aerial parts of Naravelia zeylanica dc. IJPSR


Jenkins GL, Knevel AM, Digangi FE. Quantitative Pharmaceutical

Chemistry. 6th ed. New Delhi: CBS Publishers Pvt. Ltd.; 2008.

p. 235‑80.



  • There are currently no refbacks.