Preparation And Evaluation of Hydrogel Formulation Containing Ocimum Sanctum Leaves Extract for Anti-Inflammatory Activity

Dr. Alok Pal Jain


Aim: Aim of the present study was to evaluate anti-inflammatory effect of Ocimum sanctum leaves in the
formulation of hydrogel using different types of animal models. Materials and Methods: Ethanolic extract of
O. sanctum leaves was fractionated with methanol to get methanolic fraction. Methanolic fraction (1% vw/w) was
used for hydrogel formulation with different combination of Carbopol 940 and Sodium CMC. Prepared hydrogel
was characterized for optimum physical characters, pH, spreadability, homogeneity, viscosity, release profile, and
irritation effect. Optimized hydrogel was evaluated for anti-inflammatory effect using xylene-induced, croton oilinduced
ear edema, and cotton pellet-induced granuloma model in animals. Effect was monitored by measurement
of percent inhibition, nitric oxide (NO), and myeloperoxidase (MPO) levels in mice ears after acute inflammation
induced by croton oil. Results and Discussion: Physical evaluation confirmed the color of the prepared hydrogels
was brownish and appearance was homogeneous and smooth on application. All other evaluation parameters,
for example, pH, viscosity, spreadability, and consistency were found suitable in F3 hydrogel formulation
combination. Hence, the optimize composition of F3 formulation was observed as 1.5 g of Carbopol 940 and
1% of Sodium CMC. Percent inhibition of edema in xylene induced ear edema in mice was found comparable to
standard group of treatment (65.59%). In the present study, result indicates that prepared hydrogel hydrogel of
methanol fraction of O. sanctum possesses inhibitory effects against acute inflammation. The results showing the
ability of croton oil to induce neutrophil influx into mice ear tissue. This may be reduced by treatment with extract
hydrogels, as well as Voltaren Emulgel, reduced significantly (P<0.01) the MPO levels and NO levels in mice
ears. Conclusion: The methanol extracts was exhibits its anti-inflammatory action significantly may be due to the
presence of flavonoids in O. sanctum leaves.

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