Preliminary screening of hydrogel containing Martynia annua extract for anti-inflammatory activity

Dr. Alok Pal Jain


Objective: Aim of the present study was to investigate preliminary evaluation of hydrogel formulation of methanol fraction
of Martynia annua for anti-inflammatory activity using different types of animal models. Materials and Methods: Five
different hydrogel formulations containing extract and one control without extract were prepared according to standard
method using different proportions of Carbopol 940 and sodium carboxymethyl cellulose (CMC). Prepared hydrogel
was characterized for optimization and screened for anti-inflammatory activity using Xylene induced ear edema and
Croton oil-induced ear edema in mice. Effect was observed by measurement of percent inhibition of ear edema and
biochemical parameters, for example, nitric oxide level and myeloperoxidase (MPO) level. Results: Phytochemical
screening showed that petroleum ether extract of M. annua showed the presence of sterols, terpenoids, and fatty oils
while ethanol extract of showed the presence of glycosides, phenolic compounds, flavonoids, and amino acids. Results
of all other evaluation parameters, for example, pH, viscosity, spreadability, and consistency were found suitable
in optimized formulation as 1.5 g of Carbopol 940 and 1% of Sodium CMC for best drug release profile. Effect of
MAMFH was observed greater percentage of edema inhibition (66.67%) and was comparable to standard group of
treatment (65.59%). Results showed that MPO level of inflamed ears (inflamed control and treated only with gel base)
was significantly higher in comparison with the non-inflamed control (P < 0.01), and with hydrogel formulations.
The results showing the ability of croton oil to induce neutrophil influx into mice ear tissue. This may be reduced by
treatment with extract hydrogels, as well as Voltaren Emulgel, reduced significantly (P < 0.05) the MPO levels in mice
ears. Conclusion: Methanol fraction of M. annua was capable of inhibiting ear edema induced by xylene. It may be due
to the ability of extract to either inhibit the synthesis, release or action of xylene involved in the inflammation. Methanol
extracts was exhibits its anti-inflammatory action by inhibiting the synthesis, release or action of histamine. Significant
activity may be due to presence of flavonoids in methanol extract fraction of M. annua leaves.

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