Microwave-assisted extraction of Lupeol from Cuscuta reflexa Roxb. growing on different hosts and its quantitation by high-performance thin layer chromatography

Sunita Shailajan, Sasikumar Menon, Harshvardhan Joshi


Microwave-assisted extraction (MAE) of phytochemicals has grown as a promising technique in recent years. MAE method was
developed for extraction of Lupeol from Cuscuta reflexa Roxb. growing on different hosts. An open vessel modified microwave
system has been used for extraction of Lupeol. Various critical parameters such as use of solvent, solvent volume, power and
time of irradiation were optimized. Methanol was selected as an extraction solvent based on its high dielectric constant value
and maximum solubility of Lupeol. The solvent volume, microwave power and irradiation time were optimized to 10 mL,
160 Watts and 3 min respectively. The developed method is a good alternative to time consuming conventional extraction
Key words: Conventional extraction, Cuscuta reflexa, Lupeol, microwave-assisted extraction

Full Text:



Kapoor LD. Handbook of ayurvedic medicinal plants, 1st ed. USA:

CRC Press LLC; 2005.

Government of India. Quality standards of Indian medicinal

plants. Vol. 5. Medicinal Plant Unit. New Delhi, India: Indian

Council of Medicinal Research; 2008.

Olszanecki R, Bujak-Gizycka B, Madej J, Suski M, Wołkow PP,

Jawień J, et al. Kaempferol, but not resveratrol inhibits angiotensin

converting enzyme. J Physiol Pharmacol 2008;59:387-92.

Kim EJ, Choi CH, Park JY, Kang SK, Kim YK. Underlying

mechanism of quercetin-induced cell death in human glioma cells.

Neurochem Res 2008;33:971-9.

Bischoff SC. Quercetin: Potentials in the prevention and therapy

of disease. Curr Opin Clin Nutr Metab Care 2008;11:733-40.

Reich E, Schibli A. High-performance thin-layer chromatography

for the analysis of medicinal plants. New York: Thieme Medical

Publishers; 2006.

International Conference on Harmonisation, Q2B. Validation of

analytical procedures. Vol. 62. Geneva US FDA Federal Register;

p. 27463-7.

Gallo MB, Sarachine MJ. Biological Activities of Lupeol. Int J

Biomed Pharm Sci 2009;3:46-66.

Zhang F, Chen B, Xiao S, Yao S. Optimization and comparison of

different extraction techniques for sanguinarine and chelerythrine

in fruits of Macleaya cordata (Willd) R. Br Sep Purif Technol


Hemwimon S, Pavasant P, Shotipru A. Microwave-assisted

extraction of antioxidative anthraquinones from roots of Morinda

citrifolia. Sep Purif Technol 2007;54:44-50.

Attarde DL, Aurangabadkar VM, Belsare DP, Pal SC. Report:

Quantitative estimation of beta-sitosterol, lupeol, quercetin and

quercetin glycosides from leaflets of Soymida febrifuga using

HPTLC technique. Pak J Pharm Sci 2008;21:316-9.12.

Shailajan S, Joshi H. Optimized separation and quantification

of Pharmacologically active markers Quercetin, Kaempferol,

ß-sitosterol and Lupeol from Cuscuta reflexa Roxb. J Pharm Res


Shailajan, et al.: Microwave-assisted extraction of Lupeol and its quantitation by HPTLC

How to cite this article: Shailajan

DOI: https://doi.org/10.22377/ijgp.v5i3.205


  • There are currently no refbacks.