HPLC as an assay method for the investigation of conditions of bisoprolol extraction by organic solvents

Abstract

Introduction: Sample preparation is an important step in pharmaceutical analysis and is needed to improve the metrological characteristics of the analysis: Improving accuracy, reliability, correctness and reproducibility of the determination, expanding the range of values studied, accelerating the test, and reducing the error of the results of the analysis. The objective of this research was to select the optimal conditions for the extraction of bisoprolol fumarate by organic solvents from water solutions in dependence on pH solutions. Materials and Methods: The chromatographic analysis of bisoprolol fumarate performed on liquid chromatograph ACQUITY Arc System. Results: The extraction of bisoprolol fumarate by organic solvents from water solutions in dependence on pH solutions has been conducted. The quantitative determination of bisoprolol fumarate by high-performance liquid chromatography methods has been conducted. Conclusion: As a result of studies, we have found out that the optimal extragent is chloroform, which is extracted at pH 10 to 92.57% and methylene chloride is extracted at pH 10–90.08%. We have found that bisoprolol fumarate least extracted with hexane, so these conditions may be cleaned extracts from coextractives impurities.