Hepatoprotective activity of Amomum subulatum Roxb against ethanol-induced liver damage

Mihir Y. Parmar, Purvi Shah, Vaishali Thakkar, Tejal R. Gandhi


The hepatoprotective activity of methanolic extract of Amomum subulatum Roxb (Zingiberaceae) seeds was studied against 20 % ethanol (3.76 g/kg/days, p.o for 18 days) induced liver damage in rats. Ethanol produced significant changes in various liver parameters such as functional (thiopentone-induced sleeping time) and physical (increased liver weight and volume). It also increased the biochemical parameters such as serum glutamate oxaloacetic transaminase and glutamate pyruvic transaminase, alkaline phosphatase, total and direct bilirubin, total cholesterol, triglyceride and decreased total protein along with changes in histological parameters (damage to hepatocytes). Treatment with methanolic extract of A. subulatum (100 and 300 mg/kg/day, p.o. for 18 days) and silymarin significantly prevented the functional, physical, biochemical and histological changes induced by ethanol, indicating the recovery of hepatic cells. These results demonstrate that methanolic extract of A. subulatum seeds possessed the hepatoprotective activity.
Key words: Amomum subulatum, hepatoprotective activity, ethanol

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Singha PK, Roy S, Dey S. Protective activity of andrographolide and arabinogalactan proteins from Andrographis paniculata Nees against ethanol-induced toxicity in mice. J Ethnopharmacol 2007;111:13–21.

Sharma PC, Yelne MB, Dennis TJ, Database on medicinal plants used in ayurveda. 1st ed, Vol. 2. New Delhi: CCRAS; 2002. p. 454-61.

Warrier PK, Nambiar VP, Ramankutty C. Indian Medicinal Plants a compendium of 500 species, 1st ed, India: Orient Longman Pvt Ltd; 1994. p. 128-31.

Khandelwal KR, Practical Pharmacognosy Techniques and Experiments, 16th ed, Pune: Nirali Prakashan; 2006. p. 149-56.

OECD 2001-guideline on acute oral toxicity (AOT), Environmental health and safety monograph series on testing and adjustment, No. 425.

Handa SS, Sharma A. Heptoprotective activity of andrographolide from Andrographis paniculata against Ethanol. Indian J Med Res 1990;92:276.

Shukla V, Tiwari RK, Agarwal DP. Hepatoprotective role of Picroliv isolated from Picrohiza kurroa on alcohol induced liver necrosis. Adv Pharmacol Toxicol 2001;2:9-16.

Reitman S, Frankel S. A Colorimetric method for the determination of serum glutamate oxaloacetic transaminase and glutamate pyruvic transaminase. Am J Clin Pathol 1957;28:56-63.

Kind PR, King EJ. Estimation of plasma phosphatases by determination of hydrolyzed phenol with antipyrine. J Clin Pathol 1954;7:322-30.

Malloy HJ, Evelyn KA. The determination of bilirubin with the photoelectric colorimeter. J Biol Chem 1937;119:481.

Kaplan A, Lavernel LS. Lipid metabolism. In Clinical chemistry: Interpretation and techniques, 2nd ed, Philadelphia: Lea Febiger; 1983. p. 333-6.

Fossati P, Lorenzo P. Serum triglycerides determined colorimeterically with an enzyme that produces hydrogen peroxide, Clin Chem 1982;28:2077-80.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1957;193:265-75.

Galighor AE, Koyloff EN, Essentials of practical micro technique, 2 ed, Philadelphia: Lea and Febiger; 1976. p. 210.

Sanford B, Gennaro AR, Eds. Remington; The science and practice of pharmacy, 19th ed. Easton, PA: Mack Publishing Company; 1995. p. 111.

Leo MA, Arai M, Hepatotoxicity of vitamin A and ethanol in rat. Gastroenterology 1982;82:194-205.

Kulkarni SK. Handbook of experimental pharmacology, 3rd ed, New Delhi: Vallabh Prakashan; 1999. p. 115-6.

Kumary AL, Vijayammal PL. Additive effect of alcohol and nicotine on lipid metabolism in rats. Indian J Exp Biol 1993;31:270-4.

Vilstrup H, Tygstrup N. Glucoregulation in acute liver failure. Eur J Clin Investig 1985;16:193-7.

Blancho CD, Gentil S. Alterations of glucose metabolism in chronic liver disease. Diabetes Res Clin Pract 1990;8:29-36.

Deb AC. Fundamentals of biochemistry, 7th ed. Kolkata, India: New Central Book Agency; 2002. p. 139-40.

Younes M, Strubelt O, Alcohol induced hepatotoxicity: A role for oxygen free radicals. Free Radiac Res Common 1987;3:19-26.

Lieder CS, DeCarli LM, Hepatic Microsomal ethanol oxidzing system: In vitro characterisitcs and adapative properties in vivo. J Biochem 1970;245: 2505-12.

Fromenty B, Pessayre D. Impaired mitochondrial function in microvesicular steatosis: Effect of drugs, ethanol, hormones and cytokines, J Hepatol 1997;26:43-53.

McClain CJ, Kromhount JP. Potentiation of acetaminophen hepatotoxicity by alcohol. JAMA 1980;244:251-3.

Seeff LB, Adler E. Acetaminophen hepatotoxicity in alcoholics: A therapeutic misadventure. Ann Intern Med 1986;104:399-404.

Maneesh M, Jayalakshmi H, Effect of ascorbic acid, alpha tocopherol, lecithin and L-ornithine-L-asparate on ethanol induced hypoproteinemia and hyperlipidaemia in rats. Indian J Physiol Pharmacol 2005;49:422-6.

Defeudis FV, Papadopoulos V, Drieu K, Ginkgo biloba extracts and cancer: A research area in its infancy. Fundam Clin Pharmacol 2003;17:405-17.

Takeoka GR, Dao LT. Antioxidant constituent of almond [Prunus dulcis (Mill). Webb DA] Hulls. J Agric Food Chem 2003;51:496-501.

Quyang MA, He ZD, Wu CL. Antioxidative activity of glycosides from Ligustrum sinense. Nat Prod Res 2003;17:381.

Hikino H, Tohkin M, Kiso Y, Namiki T, Nishimura S, Takeyama K, Antihepatoxic actions of Allium sativum bulbs. Planta Med 1986;53:163.

DOI: https://doi.org/10.22377/ijgp.v3i3.95


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