Anticancer-cytotoxic activity of saponins isolated from the leaves of Gymnema sylvestre and Eclipta prostrata on HeLa cells

Venkatesan Gopiesh Khanna, Krishnan Kannabiran


The anticancer-cytotoxic activities of isolated saponins, gymnemagenol (C30 H50 O4) from Gymnema sylvestre and dasyscyphin C (C28 H40 O8) from Eclipta prostrata leaves were tested under in vitro conditions in HeLa cells. The gymnemagenol and dayscyphin C at 50 μg/ml showed a good cytotoxic activity (63% and 52%, respectively) in HeLa cells at 48 hours with the IC50 value of 37 and 50 μg/ ml, respectively. 5-Fluorouracil (5-FU), a positive control, showed 57.5 % cell death with the IC50 value of 36 μg/ml. The percentage
of HeLa cell death was maximum (73%) after 96 hours with gymnemagenol, whereas dasyscyphin C showed only 53%. The isolated saponins were not toxic to Vero cells. From this study, it can be concluded that the saponins, gymnemagenol, and dayscyphin C have significant anticancer-cytotoxic activity on HeLa cells under in vitro conditions.
Key words: Gymnema sylvestre, Eclipta prostrata, anticancer cytotoxic activity, HeLa cells

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