Anticancer-cytotoxic activity of saponins isolated from the leaves of Gymnema sylvestre and Eclipta prostrata on HeLa cells

Venkatesan Gopiesh Khanna, Krishnan Kannabiran

Abstract


The anticancer-cytotoxic activities of isolated saponins, gymnemagenol (C30 H50 O4) from Gymnema sylvestre and dasyscyphin C (C28 H40 O8) from Eclipta prostrata leaves were tested under in vitro conditions in HeLa cells. The gymnemagenol and dayscyphin C at 50 μg/ml showed a good cytotoxic activity (63% and 52%, respectively) in HeLa cells at 48 hours with the IC50 value of 37 and 50 μg/ ml, respectively. 5-Fluorouracil (5-FU), a positive control, showed 57.5 % cell death with the IC50 value of 36 μg/ml. The percentage
of HeLa cell death was maximum (73%) after 96 hours with gymnemagenol, whereas dasyscyphin C showed only 53%. The isolated saponins were not toxic to Vero cells. From this study, it can be concluded that the saponins, gymnemagenol, and dayscyphin C have significant anticancer-cytotoxic activity on HeLa cells under in vitro conditions.
Key words: Gymnema sylvestre, Eclipta prostrata, anticancer cytotoxic activity, HeLa cells

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References


Gucl ustundag O, Mazza G. Saponins: Properties, applications

and processing. Crit Rev Food Sci Nutr 2007;47:231-58.

Huang W, Jia W. Use of ginsenosides Rh2 and Rg3, and aglycon

ginsenosides for the prevention of cancer. WO Patent Application

/034963 A1.

Kerwin SM. Soy saponins and the anticancer effects of soybeans

and soy-based foods. Curr Med Chem Anticancer Agents

;4:263-72.

Hsu YL, Kuo PL, Lin CC. The proliferative inhibition and apoptotic

mechanism of Saikosaponin D in human non-small cell lung cancer

A549 cells. Life Sci 2004;75:1231–42.

Kapoor LD. CRC Handbook of Ayurvedic Medicinal Plants. CRC

Press: Boca Raton, FL; 1990. p. 200-1.

khanna Gopiesh V, Kannabiran K, Larvicidal. Effect of hemidesmus

indicus, gymnema sylvestre, and eclipta prostrata against culex

quinquifaciatus mosquito larvae. Afr J Biotechnol 2007;6:307-11.

Sandhya B, Thomas S, Isabel W, Shenbagarathi R. Ethanomedicinal

plants used by the valaiyan community of piranmalai hills

(reserved forest), Tamil Nadu, India: A pilot study. Afr J Trad

Compl Alter Med 2006;3:101-14.

Gopiesh Khanna V, Kannabiran K. Antimicrobial activity of

saponin fractions of the leaves of Gymnema sylvestre and Eclipta

prostrata. World J Microbiol Biotechnol DOI 10. 1007/s 11274-008-

-7.

Yan W, Ohtani K, Kasai R, Yamasaki K. Steroidal saponin from

fruits of Tribulus terrstris. Phytochemistry 1996;42:1417-22.

Favel E, Kemertelidze M, Benidze K, Fallague, Regli P. Antifungal

activity of steroidal glycosides from Yucca gloriosa. Phytother Res

;19:158–61.

Hayakawa Y, Sasaki K, Adachi H, Furihata K, Nagai K, Shin ya

K. Thioviridamide, a novel apoptosis inducer in transformed cells

from Streptomyces olivoviridis. J Antibiotics 2006,59;1-5.

Freshney RI, Stacey, GN, Aurebach JM. Culture of specialized cells:

Culture of human stem cells, Wiley –Interscience, New Jersey: 141.

Yogeeswari P, Sriram D. Betulinic acid and its derivatives: A review

on their biological properties, Current Medicinal Chemistry

;12:657–66.

Huang D, Qi DF. Dammarane sapogenins, their use as anti-cancer

agents, and a process producing same. US Patent 2005;6:888.

Ye W, Liu X, Zhang Q, Che CT, Zhao S. Antisweet saponins from

Gymnema sylvestre. J Nat Prod 2001;64:232-5.




DOI: http://dx.doi.org/10.22377/ijgp.v3i3.89

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