Gas chromatography−Mass spectrometry analysis and antibacterial activity of essential oil from aerial parts and roots of Anisomeles indica Linn

Yogesh V. Ushir, Anil U. Tatiya, Sanjay J. Surana, Ulhas K. Patil


The essential oil from the aerial part and roots of Anisomeles indica Linn. from the Toranmal forest, Maharashtra (India), was obtained by hydrodistillation after drying and grounding of the herbal material. A total of 27 components were resolved in the essential oil by GC-MS analysis, while 18 compounds were identified comparing the recorded mass spectra with the Nist 98/Nbs 75 K GC-MS library of mass spectra. The microbiological activity of the isolated essential oils was investigated as well. It was found that the essential oil shows microbiological activity on Escherichia coli, Pseudomonas aeruginosa, Bacillus pumilus and Staphylococcus aureus with a range of minimum inhibitory concentration values extended from 31.25 to 250 μg/ml. The percentage inhibition values calculated also support the minimum inhibitory concentration findings. The MBCs also performed for the same pathogens. Limonene, pinene,
isobornyl acetate and eugenol can be considered as the main antimicrobial constituents in the essential oils of plants.
Key words: Anisomeles indica Linn, essential oil, microbiological activity

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