Antimicrobial potential of Daruharidra (Berberis aristata DC) against the pathogens causing eye infection

Tamilselvi Saravanakumar, Padma Venkatasubramanian, N. S. Vasanthi, E. Manonmani


Aim: The present work was carried out to study the antimicrobial potential of the methanolic stem extracts of authentic Daruharidra and four of its substitute plant materials along with eight of those collected from different raw drug markets in South India against six eye‑infecting bacteria viz., Nocardia sp., Staphylococcus aureus, Streptococcus pneumonia, Pseudomonas aeruginosa, Staphylococcus viridians and Escherichia coli. Materials and Methods: The entire testing was carried out by agar well diffusion method after obtaining the samples from clinical laboratory in Coimbatore. Care was taken for complete maintenance of sterile conditions. Statistical Analysis: Statistical evaluations were conducted by ANOVA and P < 0.01 were considered significant. Results: Of the six organisms tested, the extracts showed activity against only three eye infecting pathogens. The highest antimicrobial activity was
found against E. coli (24.00 ± 0) with an MIC of 23.20 mg/ml and the least against Nocardia sp (6.2 ± 0) with an MIC of 6.00 mg/ml.The inhibitory effects of the methanolic extracts on the growth of three organisms viz., Nocardia sp, S. pneumonia and E. coli are close and are comparable with the standard antibiotic discs used. Conclusion: Thus this study suggests that Daruharidra and its traded substitutes may be used in treating eye infection caused by Nocardia sp, S. pneumonia and E. coli.
Key words: Antimicrobial activity, Daruharidra, eye infection, methanolic stem extract

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