Antidiabetic effect of matured fruits of Diospyros peregrina in alloxan-induced diabetic rats

Saikat Dewanjee, Sekhar K. Bose, Ranabir Sahu, Subhash C. Mandal


Th e methanol extract of matured fruits of Diospyros peregrina was evaluated for its antidiabetic activity in alloxan-induced diabetic rats. It was also intended to establish correlation with reduction of oxidative state associated with diabetes. Diabetes was induced by a single intraperitoneal injection of alloxan monohydrate (150 mg/kg body weight). Methanol extract was administered orally at doses of 150 and 300 mg/kg body weight for 14 consecutive days in diabetic rats. Fasting blood glucose level, serum lipid profi les, iver glycogen level, pancreatic thiobarbituric acid reactive substances (TBARS) as well initial and fi nal changes in body weight were evaluated. Experimental fi ndings showed signifi cant antidiabetic potential of extract in terms of reduction of fasting blood glucose
level in diabetic rats. Observed data was found statistically signifi cant in reduction of serum lipid and pancreatic TBARS levels whilst signifi cant improvement was observed in liver glycogen level and body weight profi les in extract-treated diabetic rats. Th e eff ect of the extract particularly at 300 mg/kg was comparable to that of standard drug glibenclamide (1 mg/kg body weight).
Key words: Alloxan, antihyperglycemic, diabetes, Diospyros peregrina, glibenclamide

Full Text:



Tiwari AK, Rao JM. Diabetes mellitus and multiple therapeutic

approaches of phytochemicals: Present status and future prospects.

Curr Sci 2002;83:30-8.

Kaczmar T. Herbal support for diabetes management. Clin Nutr

Insights 1998;6:1-4.

Rao KN, Krishna MB, Srinivas N. Eff ect of chronic administration

of Boerhaavia diff usa Linn.leaf extract on experimental diabetes in

rats. Trop J Pharma Res 2004;3:305-9.

Bailey CJ, Day C. Traditional treatments for diabetes. Diabetes

Care 1989;12:553-64.

Rahman AU, Zaman K. Medicinal plants with hypoglycemic

activity. J Ethnopharmacol 1989;26:1-55.

Valiathan MS. Healing plants. Curr Sci 1998;75:1122-6.

Momin A. Role of indigenous medicine in primary health care.

In: Proceedings of First International seminar on Unani Medicine.

New Delhi, India: 1987. p. 54.

Kirtikar KR, Basu BD. Indian Medicinal Plants, Reprint edition,

Vol 2. In: Singh B, Singh MP, editors. Deharadun: 1975. p. 1502-4.

Anjaria J, Parabia M, Bhatt G, Khamar R. A Glossary of selected

indigenous medicinal plants of India. 2nd ed. Ahmedabad, India:

SRISTI Innovations; 2002. p. 26.

Asolkar LV, Kakkar KK, Chakre OJ. Second supplement to Glossary

of Indian Medicinal Plants with Active Principles Part-I (A-K). 1st

ed. Part 1. New Delhi: CSIR (PID); 1992. p. 279.

Chopra RN, Nayar SL, Chopra IC. Glossary of Indian Medicinal

Plants. 1st ed. 3rd reprint. New Delhi: CSIR; 1992. p. 99.

Jain N, Yadav RN. Furano-(2,3,7,8)-3,5-dimethoxy-5-

hydroxyß avone: A new furanoß avone from the fruits of Diospyros

peregrina Gurke. Asian J Chem 1997;9:442-4.

Misra PS, Misra G, Nigam SK, Mitra CR. Constituents of Diospyros

peregrina fruit and seed. Phytochemistry 1971;10:904-5.

Jain N, Yadav RN. Peregrinol, a lupane type triterpene from the

fruits of Diospyros peregrine. Phytochemistry 1994;35:1070-2.

Ghani, A, Medicinal Plants of Bangladesh. 1st ed. Dhaka: Asiatic

Society of Bangladesh; 1998. p. 164.

Kokate CK. Practical Pharmacognosy. New Delhi, India: Vallabh

Prakashan; 1994. p. 107-10.

PHS (Public Health Service), Public Health Service Policy on

Humane Care and Use of Laboratory Animals, Washington, D.C.,

U.S., Department of Health and Human Services, Available from

Offi ce for Protection from Research Risks, Building 31, Room 4B09.

Bethesda: NIII; 1986.

Kastumata K, Kastumata Y, Ozawa T, Kastumata K. Potentiating

eff ect of combined usage of three sulfonylurea drugs on the

occurrence of alloxan diabetic rats. Hormone Metab Res


Mandal SC, Mukharjee PK, Saha K, Das J, Pal M, Saha BP.

Hypoglycemic activity of Ficus racemosa L. (Moraceae) Leaves in

streptozotocin induced Diabetic Rats. Nat Prod Sci 1997;3:38-41.

Caroll NV, Longley RW, Roe JH. The determination of glycogen

in liver and muscle by use of anthron reagent. J Biol Chem


Hiroshi O, Nobuko O, Kunio V. Assay for lipid peroxides in

animal tissues by thiobarbituric acid reaction. Anal Biochem


Shirwaikar A, Rajendran K, Kumar CD. Oral antidiabetic activity

of Annoa squamosa leaf alcohol extract in NIDDM rats. Pharma Biol


Omamoto H, Ucgigata Y, Hiroskitckan ST. Alloxan induces DNA

strand breaks and poly ADPribose synthase in pancreatic islets.

Nature 1981;294:284-6.

Chase PH, Glasgow AM. Juvenile diabetes mellitus and serum

lipid and lipoprotein levels. Am J Dis Child 1976;130:1113-7.

Swanston Flatt SK, Day C, Bailey CJ, Flatt PR. Traditional plant

treatments for diabetes: Studies in normal and streptozotocin

diabetic mice. Diabetologia 1990;33:462-4.

Huang X, Vaag A, Hanson M, Weng, Goop L. Impaired insulin

stimulated expression of the glycogen synthase gene in skeletal

muscle of type II diabetic patient is acquired rather than inherited.

J Clin Endocrinol Metab 2000;85:1584-90.

Halliwell B, Gutt erdge JM. Free radicals in biology and medicine.

London: Oxford Claredon Press; 1985. p. 24-86.

Olmedilla MN. Reference values for retinal, tochopherol and main

carotinoids in serum of control and insulin dependent diabetic

Spanish subject. Clin Chem 1999;43:1066-71.

Miura T, Ichhiki H, Hashimoto I, Iwamoto N, Kato M, Kubo M,

et al. Antidiabetic activity of xanthone compound, mangiferin.

Phytomedicine 2001;8:85-7.



  • There are currently no refbacks.