Total antioxidant capacity using ferric reducing antioxidant power and 2, 2-diphenyl-1 picryl hydrazyl methods and phenolic composition of fresh and dried drumstick (Moringa oleifera) leaves

Vanisha S. Nambiar, Hema M. Matela, Alma Baptist

Abstract


Context: Dietary and life‑style transitions have instigated accumulation of free radicals in our body causing oxidative stress, which is etiological in Non Communicable Diseases. Antioxidants are the means to counter oxidative stress. Thus, comprehensive knowledge of potential sources of antioxidants is vital. Aim: To assess the total antioxidant capacity (TAC) using ferric reducing antioxidant power (FRAP), 2,2‑diphenyl‑1‑picrylhydrazyl (DPPH) assays and phenolic composition of fresh and dried drumstick leaves from
Western India. Settings and Design: This was an experimental study conducted under laboratory settings. Samples were procured from three different parts of the city of Vadodara, Western India. Materials and Methods: Fresh, oven‑dried, and air‑dried form of leaves were quantified for total phenol content (TPC) (Folin Ciocalteau assay), individual phenols (high performance liquid chromatography). Reducing property and free radical inhibiting property were quantified to understand the TAC using FRAP and DPPH assays respectively. Statistical Tests: Regression analyses were used for TPC and FRAP calculations. Results: The TPC ranged from 141.59 mgGAE/100 g to 185.32 mgGAE/100g; FRAP levels ranged from 0.49 mg/g to 0.7 mg/g, and DPPH ranged from 4.5% to 12.99% among raw and blanched Drumstick leaves sample in fresh, air‑dried, and oven‑dried form. High Pressure Liquid Chromatography analysis revealed the presence of gallic acid, epigallocatechingallate, chlorogenic acid. Conclusion: The underutilised
drumstick leaves have strong antioxidant capacity attributable to its phenolic composition. Thus, they are cheap and accessible source of antioxidants for medicinal and commercial purposes.
Key words: Antioxidants, drumstick leaves, ferric reducing antioxidant power, Moringa oleifera, total antioxidant capacity,
2,2‑diphenyl‑1‑picrylhydrazyl

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References


Sies H. Polyphenols and health: Update and perspectives. Arch

Biochem Biophys 2010;501:2‑5.

Keaney JF. Oxidative Stress and Vascular Disease.: Boston: Kluwer

Academic Publishers; 2000. p. 1‑8.

FSA. Eatwell: 8 tips for making healthier choices, 2010. Available

from: http://www.food.gov.uk/multimedia/pdfs/publication/

eatwell0708.pdf. Last accessed on 2012 Jan 27].

Bhat PN. Nutrition and Anemia. NFHS III. National Family Health

Survey 2005‑06. Mumbai: International Institute for Population

Sciences (IIPS) and Macro International; 2007. p. 299 .

Alwan A. Chp‑1 Burden: Mortality, morbidity and risk factors.

WHO Global Status Report on Non Communicable Diseases. Italy:

WHO Press; 2010. p. 20.

Jaenicke H and Hosschle‑Zeledon I (editors). Introduction IPGRI.

Strategic Framework for Underutilized Plant Species Research

and Development, with Special Reference to Asia and the Pacific,

and to Sub‑Saharan Africa. Rome, Italy: International Centre for

Underutilised Crops, Colombo, Sri Lanka and Global Facilitation

Unit for Underutilized Species, 2006. p. 4 .

Executive Summary, Neglected and Underutilized Plant Species:

Strategic Action Plan of the International Plant Genetic Resources

Institute. Rome, Italy: International Plant Genetic Resources

Institute; 2002. p. 5 .

Nambiar VS, Seshadri S. Beta carotene content of some green

leafy vegetables of Western India by HPLC. J Food Sci Technol

;35:365‑7.

Nambiar VS, Seshadri S. Bioavailability trials of beta‑carotene from

fresh and dehydrated drumstick leaves (Moringa oleifera) in a rat

model. Plant Foods Hum Nutr 2001;56:83‑95.

Singleton VL, Rossi JA. Colorimetry of total phenolics with

phosphomolybdic‑phosphotungstic acid reagent. Am J Enol Vitic

;16:144‑58.

Waterhouse AL. Determination of Total Phenolics. Current

Protocols in Food Analytical Chemistry. Davis, California: John

Wiley and Sons, Inc; 2002. p. 7.

Apak R, Güçlü K, Demirata B, Ozyürek M, Celik SE, Bektaşoğlu B,

et al. Comparative evaluation of various total antioxidant capacity

assays applied to phenolic compounds with the CUPRAC assay.

Molecules 2007;12:1496‑547.

Halvorsen BL, Carlsen MH, Phillips KM, Bøhn SK,

Holte K, Jacobs DR Jr, et al. Content of redox‑active

compounds (i.e., antioxidants) in foods consumed in the United

States. Am J ClinNutr 2006;84:95‑135.

Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as

a measure of “antioxidant power”: The FRAP assay. Anal Biochem

;239:70‑6.

Brand‑Williams W, Cuvelier ME, Berset C. Use of free radical

method to evaluate antioxidant activity. Lebenson Wiss Technol

;28:25‑30.

Kaur C. Kapoor HC.Antioxidants in fruits and vegetables‑the

millenium’s health. Int J Food Sci Technol 2001;36:703‑25.

Benavente‑Garcia O, Castillo J, Marin FR, Ortuno A, Rio JA. Uses and

properties of citrus flavonoids. J Agric Food Chem 1997;45:4505‑15.

Manach C, Williamson G, Morand C, Scalbert A, Rémésy C.

Bioavailability and bioefficacy of polyphenols in humans. I. Review

of 97 bioavailability studies. Am J ClinNutr 2005;81:230S‑242S.

Middleton E Jr, Kandaswami C, Theoharides TC. The effects of plant

flavonoids on mammalian cells: Implications for inflammation,

heart disease, and cancer. Pharmacol Rev 2000;52:673‑751.

Puupponen‑Pimiä R, Nohynek L, Meier C, Kähkönen M,

Heinonen M, Hopia A, et al. Antimicrobial properties of phenolic

compounds from berries. J ApplMicrobiol 2001;90:494‑507.

Heo HJ, Kim YJ, Chung D. Antioxidant capacities of individual and

combined phenolics in a model system. Food Chem 2007;104:87‑92.

Lako J, Trenerry CV, Wahlqvist M, Wattanapenpaiboon N.

Phytochemical avonols, carotenoids and the antioxidant properties

of a wide selection of Fijian fruit, vegetables and other readily

available foods. Food Chem 2007;101:1727-41.

Verma AR, Vijayakumar M, Mathela CS, Rao CV. In vitro and

in vivo antioxidant properties of different fractions of Moringa

oleifera leaves. Food ChemToxicol 2009;47:2196‑201.

Siddhuraju P, Becker K. Antioxidant properties of various

solvent extracts of total phenolic constituents from three different

agroclimatic origins of drumstick tree (Moringa oleifera Lam.)

leaves. J Agric Food Chem 2003;51:2144‑55.

Bajpai M, Pande A, Tewari SK, Prakash D. Phenolic contents and

antioxidant activity of some food and medicinal plants. Int J Food

SciNutr 2005;56:287‑91.

Lim BO, Yu BP, Cho SI, Her E, Park DK. The inhibition by

quercetin and ganhuangenin on oxidatively modified low density

lipoprotein. Phytother Res 1998;12:340‑5.

Erlejman AG, Verstraeten SV, Fraga CG, Oteiza PI. The interaction

of flavonoids with membranes: Potential determinant of flavonoid

antioxidant effects. Free Radic Res 2004;38:1311‑20.

Rao BN. Bioactive phytochemicals in Indian foods and their

potential in health promotion and disease prevention. Asia Pac J

ClinNutr 2003;12:9‑22.

Sunan W, John PM, Rong T, Massimo FM. How natural dietary

antioxidants in fruits, vegetables and legumes promote vascular

health. Food Res Int 2011;44:14‑22.

Chen L, Yang X, Jiao H, Zhao B. Tea catechins protect against

lead‑induced cytotoxicity, lipid peroxidation, and membrane

fluidity in HepG2 cells. ToxicolSci 2002;69:149‑56.

Kim HY, Kim OH, Sung MK. Effects of phenol‑depleted and

phenol‑rich diets on blood markers of oxidative stress, and urinary

excretion of quercetin and kaempferol in healthy volunteers. J Am

CollNutr 2003;22:217‑23.

Chen YT, Li XJ, Zao BL, Zheng RL, Xin WJ. ESR study of the

scavenging effect of natural products of rutin etc., on superoxide

and hydroxyl radical. Acta Biophys 1989;5:235‑40.

Wong CC, Li HB, Cheng KW, Chen F. A systematic survey of

antioxidant activity of 30 Chinese medicinal plants using the ferric

reducing antioxidant power assay. Food Chem 2006;97:705‑11.

Tzulker R, Glazer I, Bar‑Ilan I, Holland D, Aviram M, Amir R.

Antioxidant activity, polyphenol content, and related compounds

in different fruit juices and homogenates prepared from 29 different

pomegranate accessions. J Agric Food Chem 2007;55:9559‑70.

Katalinic V, Milos M, Kulisic T, Jukic M. Screening of 70 medicinal

plant extracts for antioxidant capacity and total phenols.Food

Chem 2006;94:550‑7.




DOI: http://dx.doi.org/10.22377/ijgp.v7i1.300

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